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Biobricks construction

• PCR to obtain linearised pSB1C3, using linearised pSB1C3 as template, with the protocol from iGEM parts registry. Didn't work.
• PCR on ParD and ParE to have those genes outside of a plasmid (as PCR products). Worked. We used a plasmid containing both genes as template.
• PCR on FLP, using the sequence we got synthesized as backbone, to add RCF 10 prefix and suffix to the gene. Didn't work.
• Digestion of Cm and Kan resistance cassettes with RCF10 enzymes in order to insert them in pSB1C3. Worked.

Module hydrogen

• We obtained bacteria strains with two deleted genes: one strain that lacked LdhA and FocA and the other one was missing LdhA and PPC.