Team:METU Turkey/Results Discussion/Gel Extraction

From 2010.igem.org

Revision as of 23:26, 27 October 2010 by Cerenseref (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

MATERIALS

UV lamp - B58
Heat block - M3
Centrifuge - M8

CHECK-LIST PROCEDURE

GeneJET™ Gel Extraction Kit #K0692 procedure

NOTES

- Gel is cut under UV lamp.
- While the gel is being cut, it shouldn’t be exposed to UV much otherwise DNA mutations may occur and mutated DNA wouldn’t be appropriate to use in cloning.
- While cutting the gel, excess amount of gel should be removed.
- Before putting the gel piece into the eppendorf, empty eppendorf should be weight so that it can be omitted during the weighing of the gel.
- Before starting gel extraction, heat block should be set at 55C.