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User contributions
From 2010.igem.org
- 03:50, 28 October 2010 (diff | hist) Team:METU Turkey/Results Discussion/Ethanol precipitation (top)
- 03:42, 28 October 2010 (diff | hist) Team:METU Turkey/Results Discussion/Protein expression (top)
- 03:40, 28 October 2010 (diff | hist) Team:METU Turkey/Results Discussion/Protein expression
- 03:38, 28 October 2010 (diff | hist) Team:METU Turkey/Results Discussion/Protein expression
- 03:36, 28 October 2010 (diff | hist) Team:METU Turkey/Results Discussion/Transformation (top)
- 02:45, 28 October 2010 (diff | hist) Team:METU Turkey/Results Discussion/Isothermal Titration Calorimetry (ITC) (top)
- 02:44, 28 October 2010 (diff | hist) Team:METU Turkey/Results Discussion/Isothermal Titration Calorimetry (ITC)
- 00:06, 28 October 2010 (diff | hist) Talk:Team:METU Turkey/Safety (top)
- 00:06, 28 October 2010 (diff | hist) N Talk:Team:METU Turkey/Safety (New page: <br>- All experiments listed in this project were conducted under standard laboratory safety rules. <br>- In experiments where CO feeding was required, a sensitive electronic carbon mon...)
- 23:26, 27 October 2010 (diff | hist) N Team:METU Turkey/Results Discussion/Gel Extraction (New page: <br>MATERIALS <br>UV lamp - B58 <br>Heat block - M3 <br>Centrifuge - M8 <br>CHECK-LIST PROCEDURE <br>GeneJET™ Gel Extraction Kit #K0692 procedure <br>NOTES <br>- Gel is cut under ...) (top)
- 22:55, 27 October 2010 (diff | hist) Team:METU Turkey/Results Discussion/Cell Imaging (top)
- 21:30, 27 October 2010 (diff | hist) Team:METU Turkey/Results Discussion/Plasmid purification (top)
- 21:28, 27 October 2010 (diff | hist) Team:METU Turkey/Results Discussion/Plasmid purification
- 21:27, 27 October 2010 (diff | hist) Team:METU Turkey/Results Discussion/Plasmid purification
- 21:22, 27 October 2010 (diff | hist) N Team:METU Turkey/Results Discussion/Plasmid purification (New page: <br>MAIN STEPS/TIME TABLE <br>- Incubation [12-14 h] <br>- Plasmid purification [30-45 min] <br>MATERIALS <br>- FERMANTAS GeneJET™ Plasmid Miniprep Kit (#K0502 or #K0503) <br>...)
- 21:04, 27 October 2010 (diff | hist) Nm Team:METU Turkey/Results Discussion/Glycerol stock preparation (New page: <br>MATERIALS <br>- SOB/LB Media <br>- Glycerol (100%) <br>- Sterile Milli-Q dH2O <br>- Liquid nitrogen <br>CHECK-LIST PROCEDURE <br>- Choose colony put into 5 mL of LB medium in 15 m...)
- 21:01, 27 October 2010 (diff | hist) Team:METU Turkey/Results Discussion/Isothermal Titration Calorimetry (ITC) (Removing all content from page)
- 21:01, 27 October 2010 (diff | hist) N Team:METU Turkey/Results Discussion/Cell Imaging (New page: <br>CLSM IMAGING <br>- Take 10mL from the samples (OD=1-1.9) that are dissolved in phosphate buffer solution. <br>- Spread the samples on the slide with the help of an inoculation loop....)
- 20:58, 27 October 2010 (diff | hist) Team:METU Turkey/Results Discussion/Isothermal Titration Calorimetry (ITC)
- 20:57, 27 October 2010 (diff | hist) Team:METU Turkey/Results Discussion/Isothermal Titration Calorimetry (ITC)
- 20:56, 27 October 2010 (diff | hist) N Team:METU Turkey/Results Discussion/Isothermal Titration Calorimetry (ITC) (New page: <br>CLSM IMAGING <br>- Take 10mL from the samples (OD=1-1.9) that are dissolved in phosphate buffer solution. <br>- Spread the samples on the slide with the help of an inoculation loop. ...)
- 20:55, 27 October 2010 (diff | hist) Team:METU Turkey/Results Discussion/PCR
- 20:53, 27 October 2010 (diff | hist) N Team:METU Turkey/Results Discussion/PCR (New page: <br>MAIN STEPS/ TIME TABLE <br>- Each step is carried out for 1 tube <br>- PCR solution preparation [30-45 min] <br>- Thermocycler [2.5 h] <br>- Denaturation <br>- Annealing <b...)
- 20:47, 27 October 2010 (diff | hist) Team:METU Turkey/Results Discussion/LIGATION
- 20:46, 27 October 2010 (diff | hist) N Team:METU Turkey/Results Discussion/LIGATION (New page: <br>CHECK-LIST PROCEDURE <br>Add; <br>- uL nuclease free water <br>- uL rapid DNA ligation buffer <br>- uL T4 DNA ligase <br>- uL from 25uL insert1(downstream) elute (elution must be d...)
- 20:43, 27 October 2010 (diff | hist) N Team:METU Turkey/Results Discussion/Agarose Gel Electrophoresis (New page: <br>MAIN STEPS/TIME TABLE <br>- 1% gel preparation [30 min] <br>- Running [1 h] <br>- Visualization [15 min] <br>MATERIALS <br>- Electrophoresis tank <br>- Power supply <br>- Transillumin...)
- 20:38, 27 October 2010 (diff | hist) Team:METU Turkey/Results Discussion/PCR Purification
- 20:36, 27 October 2010 (diff | hist) N Team:METU Turkey/Results Discussion/PCR Purification (New page: <br>MATERIALS <br>- Heating block <br>- Microcentrifuge <br>- PCR Purification Kit <br>- Binding Buffer <br>- Wash Buffer <br>- Elution Buffer <br>- Water nuclease free ...)
