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Team:METU Turkey/Results Discussion/Isothermal Titration Calorimetry (ITC)
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Revision as of 20:57, 27 October 2010 by Cerenseref (Talk | contribs)
CLSM IMAGING
- Take 10mL from the samples (OD=1-1.9) that are dissolved in phosphate buffer solution.
- Spread the samples on the slide with the help of an inoculation loop.
- Wait for the sample to dry.
- Pass the slide through the flame 3-4 times, being careful to have the cells on the upper part of the slide.
- Observe the slides under Brightfield 100X
-Settings for GFP:
HFT 488
Ch 1
LP 505
Line 8
543 nm excitation
Laser power 5%
- Settings for RFP:
HFT 488/543
Ch 1
LP 560
Line 8
543 nm excitation
Laser power 10%
