Team:Warsaw/Calendar-Stage1/8 September 2010
From 2010.igem.org
07.09.2010 Tuesday
Testing the fluorescence of GFP in different pH in a microfluidic systemm by using flow cytometry[Ania O.,Cherry]
1. Enclosing GFP in different pH buffers into droplets in a water-in-oil-in-water emulsion
2. Preparation of probes and examining them in flow cytometer
Data analysis with Cell Quest[Cherry]
legend:
07-09-2010.004- pH 7
07-09-2010.005- pH 8
07-09-2010.006- pH 6,8
07-09-2010.007- pH 4,8
07-09-2010.010- pH 8
Data from Cell Quest analysis:
![](https://static.igem.org/mediawiki/2010/d/db/Rbs.7.09_01.png)
![](https://static.igem.org/mediawiki/2010/8/8b/Rbs.7.09_02.png)
![](https://static.igem.org/mediawiki/2010/c/cf/Rbs.7.09_03.png)
![](https://static.igem.org/mediawiki/2010/c/c3/Rbs.7.09_04.png)
![](https://static.igem.org/mediawiki/2010/4/4d/Rbs.7.09_05.png)
![](https://static.igem.org/mediawiki/2010/6/6c/Rbs.7.09_06.png)