| mi/shRNA-name | sequence | BSsequence | number of BS
| perfect
| bulged
| bulge size
| seed type
| 3' score
| AU score
| knockdown [%] P
| experi- mental conditions
| knockdown [%] N
|
| MiR122_99 | TGGAGTGTGACAATGGTGT TTGT | GACAAACACCATTGTCACA CTCCA | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | |
|
| MiR122_101 | TGGAGTGTGACAATGGTGT TTGT | GACAAACACCATGAAGACA CTCCA | 1 | 0 | 1 | 4 | 3 | 7.5 | 0.624 | 0.85 | | 0.21
|
| MiR122_105 | TGGAGTGTGACAATGGTGT TTGT | GACTTGAGCAGAACAAACA CTCCA | 2 | 0 | 1 | 4 | 3 | 7.5 | 0.576 | 0.81 | | 0.25
|
| MiR122_106 | TGGAGTGTGACAATGGTGT TTGT | GACAAACACCATACGGACA CTCCAGAGACACAAACACC ATGAAGACACTCCA | 1 | 0 | 1 | 4 | 3 | 7.5 | 0.595 | 0.92 | | 0.13
|
| MiR122_107 | TGGAGTGTGACAATGGTGT TTGT | GACTAAGGCTGCTCCATCA ACACTCCA | 1 | 0 | 1 | 4 | 3 | 7.5 | 0.597 | 0.69 | | 0.36
|
| MiR122_108 | TGGAGTGTGACAATGGTGT TTGT | GCAATGGAGAGTCACCTAG ACACTCCA | 1 | 0 | 1 | 4 | 3 | 7.5 | 0.603 | 0.08 | | 0.96
|
| MiR122_109 | TGGAGTGTGACAATGGTGT TTGT | GGCCAGCACCATTTCACAC ACACTCCT | 1 | 0 | 1 | 4 | 3 | 7.5 | 0.624 | 0.72 | | 0.33
|
| MiR122_110 | TGGAGTGTGACAATGGTGT TTGT | GCCCCTGATGGGGGCGACA CTCCA | 1 | 0 | 1 | 4 | 3 | 7.5 | 0.6 | 0.28 | | 0.76
|
| MiR122_111 | TGGAGTGTGACAATGGTGT TTGT | GACTTGAGCAGAACAAACA CTCCA | 1 | 0 | 1 | 4 | 3 | 7.5 | 0.603 | 0.58 | | 0.47
|
| MiR122_112 | TGGAGTGTGACAATGGTGT TTGT | GCAAATCATGATCAAAAAC ACTCCC | 1 | 0 | 0 | 0 | 2 | 5 | 0.336 | 0.34 | | 0.7
|
| MiR122_132 | TGGAGTGTGACAATGGTGT TTGT | GACAAACACCATGTCGACA CTCCA | 1 | 0 | 0 | 0 | 3 | 1.5 | 0.327 | 0.82 | | 0.23
|
| MiR122_133 | TGGAGTGTGACAATGGTGT TTGT | GACAAACACCATGCCAACA CTCCA | 1 | 0 | 0 | 0 | 3 | 4 | 0.314 | 0.83 | | 0.22
|
| MiR122_134 | TGGAGTGTGACAATGGTGT TTGT | GACAAACACCATACGAACA CTCCA | 1 | 0 | 0 | 0 | 3 | 2.5 | 0.314 | 0.77 | | 0.28
|
| MiR122_135 | TGGAGTGTGACAATGGTGT TTGT | GACAAACACCATGCAGACA CTCCA | 1 | 0 | 0 | 0 | 3 | 2 | 0.327 | 0.76 | | 0.29
|
| MiR122_158 | TGGAGTGTGACAATGGTGT TTGT | GACAAACACCATGCCTACA CTCCA | 1 | 0 | 0 | 0 | 2 | 2.5 | 0.221 | 0.82 | | 0.23
|
| sAg_19_bs_r10_12_acg_fw | GAACAAATGGCACTAGTAA | TTACTAGACGCATTTGTTC | 1 | 0 | 1 | 3 | 2 | 5.5 | 0.442 | | |
|
| sAg_19_bs_r10_12_taa_fw | GAACAAATGGCACTAGTAA | TTACTAGTAACATTTGTTC | 1 | 0 | 1 | 2 | 2 | 6 | 0.492 | | |
|
| sAg_19_bs_r9_12_acgg_fw | GAACAAATGGCACTAGTAA | TTACTAGACGGATTTGTTC | 1 | 0 | 1 | 4 | 2 | 5.5 | 0.442 | | |
|
| sAg_19_bs_r9_12_atgt_fw | GAACAAATGGCACTAGTAA | TTACTAGATGTATTTGTTC | 1 | 0 | 1 | 4 | 2 | 5.5 | 0.495 | | |
|
| sAg_19_bs_m10cg_fw | GAACAAATGGCACTAGTAA | TTACTAGTGGCATTTGTTC | 1 | 0 | 1 | 1 | 2 | 6.5 | 0.442 | | |
|
| sAg_19_bs_m10ca_fw | GAACAAATGGCACTAGTAA | TTACTAGTGACATTTGTTC | 1 | 0 | 1 | 1 | 2 | 6.5 | 0.496 | | |
|
| sAg_19_bs_m11gc_fw | GAACAAATGGCACTAGTAA | TTACTAGTCCCATTTGTTC | 1 | 0 | 1 | 1 | 2 | 6 | 0.442 | | |
|
| sAg_19_bs_m11ga_fw | GAACAAATGGCACTAGTAA | TTACTAGTACCATTTGTTC | 1 | 0 | 1 | 1 | 2 | 6 | 0.466 | | |
|
| sAg_19_bs_onlyseed_fw_E | GAACAAATGGCACTAGTAA | AATGATCACGGATTTGTTC | 1 | 0 | 0 | 0 | 2 | 0 | 0.442 | | |
|
| sAg_19_bs_p_fw_E | GAACAAATGGCACTAGTAA | TTACTAGTGCCATTTGTTC | 1 | 1 | 0 | 0 | 0 | 0 | 0 | | |
|
| sag_25_1 | GAACAAATGGCACTAGTAA ACTGAG | ATAATTTGTTCATTTGTTC | 2 | 0 | 0 | 0 | 2 | 1.5 | 0.491 | | |
|
| sag_25_2 | GAACAAATGGCACTAGTAA ACTGAG | ATAATTTGTTCATTTGTTC ATTTGTTC | 3 | 0 | 0 | 0 | 2 | 1.5 | 0.491 | | |
|
| sag_25_3 | GAACAAATGGCACTAGTAA ACTGAG | AGTTTACTAGTGCCATTTG TTCAAAUAUAGCC | 1 | 1 | 0 | 0 | 0 | 0 | 0 | | |
|
| sag_25_4 | GAACAAATGGCACTAGTAA ACTGAG | AGTTTACTAGTGCAATTTG TTAAAAUUUAGCC | 1 | 0 | 1 | 1 | 3 | 8.5 | 0.587 | | |
|
| sag_25_5 | GAACAAATGGCACTAGTAA ACTGAG | AGTTTACTAGTGAAATTTG TTAAAAUUUAGCC | 1 | 0 | 1 | 2 | 3 | 8 | 0.587 | | |
|
| sag_25_6 | GAACAAATGGCACTAGTAA ACTGAG | AGTTTACTAGTAAAATTTG TTAAAAUUUAGCC | 1 | 0 | 1 | 3 | 3 | 7.5 | 0.587 | | |
|
| sag_25_7 | GAACAAATGGCACTAGTAA ACTGAG | AGTTTACTAGAAAAATTTG TTAAAAUUUAGCC | 1 | 0 | 1 | 4 | 3 | 7 | 0.587 | | |
|
| sag_25_8 | GAACAAATGGCACTAGTAA ACTGAG | CTGGGCAATTATAAATTTG TTAAAAUUUAGCC | 1 | 0 | 0 | 0 | 3 | 2 | 0.603 | | |
|
| sag_25_9 | GAACAAATGGCACTAGTAA ACTGAG | CTGGGCAGCCGCAAATTTG TTAAAGGCCCGCC | 1 | 0 | 0 | 0 | 3 | 2 | 0.305 | | |
|
| sag_25_10 | GAACAAATGGCACTAGTAA ACTGAG | CTGGGCAGCTATAATTTTG TTAAAAUUUAGCC | 1 | 0 | 0 | 0 | 1 | 2.5 | 0.69 | | |
|
| sag_25_11 | GAACAAATGGCACTAGTAA ACTGAG | AGTTTACGCCGTAAATTTG TTGAAGGCCCGCC | 1 | 0 | 0 | 0 | 2 | 4 | 0.226 | | |
|
| sag_25_12 | GAACAAATGGCACTAGTAA ACTGAG | CTGGGCAATTATAAATTTG TTGAAAUUUAGCC | 1 | 0 | 0 | 0 | 2 | 2 | 0.526 | | |
|
| sag_25_13 | GAACAAATGGCACTAGTAA ACTGAG | TCCTTACTAGTGCAATTTG TTAAAGGCCCGCC | 1 | 0 | 0 | 0 | 3 | 7 | 0.305 | | |
|
| sag_25_14 | GAACAAATGGCACTAGTAA ACTGAG | CTGAATATAGTGAAATTTG TTAAAAUUUAGCC | 1 | 0 | 0 | 0 | 3 | 4 | 0.603 | | |
|
| sag_25_15 | GAACAAATGGCACTAGTAA ACTGAG | AGTTTACTACCTAATTTTG TTAAAAUCCGGCC | 1 | 0 | 0 | 0 | 1 | 4 | 0.497 | | |
|
| sag_25_16 | GAACAAATGGCACTAGTAA ACTGAG | CTGGGCCTAGTGGATTTTG TTAAAGGCCCGCC | 1 | 0 | 0 | 0 | 1 | 5 | 0.366 | | |
|
| sag_25_17 | GAACAAATGGCACTAGTAA ACTGAG | AGTTTACATTGCAAATTTG TTGAAGGCCCGCC | 1 | 0 | 0 | 0 | 2 | 4 | 0.226 | | |
|
| sag_25_18 | GAACAAATGGCACTAGTAA ACTGAG | CTGGGACTAGTGCAATTTG TTGAAGGCCCGCC | 1 | 0 | 0 | 0 | 2 | 6 | 0.226 | | |
|
| sag_25_20 | GAACAAATGGCACTAGTAA ACTGAG | AGTTTACTAGAAAATTTTG TTAAAAUUUAGCC | 1 | 0 | 0 | 0 | 1 | 7 | 0.69 | | |
|
| sag_25_21 | GAACAAATGGCACTAGTAA ACTGAG | AGTTTACTAGAAAAATTTG TTGAAAUUUAGCC | 1 | 0 | 0 | 0 | 2 | 7 | 0.526 | | |
|
| sag_25_23 | GAACAAATGGCACTAGTAA ACTGAG | CTGGGCATAGATAATTTTG TTAAAAUUUAGCC | 1 | 0 | 0 | 0 | 1 | 3 | 0.69 | | |
|
| sag_25_24 | GAACAAATGGCACTAGTAA ACTGAG | CTGGTACTAGCTAATTTTG TTAAAAUCCGGCC | 1 | 0 | 0 | 0 | 1 | 5 | 0.497 | | |
|
| sag_25_25 | GAACAAATGGCACTAGTAA ACTGAG | AGTTTACTAGCCGATTTTG TTAAAGGCCCGCC | 1 | 0 | 0 | 0 | 1 | 7 | 0.366 | | |
|
| sag_25_26 | GAACAAATGGCACTAGTAA ACTGAG | CTGGGCCTAGAAAAATTTG TTGAAAUCCGGCC | 1 | 0 | 0 | 0 | 2 | 4 | 0.348 | | |
|
| sag_25_27 | GAACAAATGGCACTAGTAA ACTGAG | CTTTTACTAGAAAAATTTG TTGAAUUUAGCC | 1 | 0 | 0 | 0 | 2 | 6 | 0.51 | | |
|
| sag_25_28 | GAACAAATGGCACTAGTAA ACTGAG | CTGGTACTAGGCAAATTTG TTGAAGGCCCGCC | 1 | 0 | 0 | 0 | 2 | 5 | 0.226 | | |
|
| sag_25_29 | GAACAAATGGCACTAGTAA ACTGAG | GCTTTACTAGAAAAATTTG TTAAAAUUUAGCC | 1 | 0 | 0 | 0 | 3 | 6 | 0.603 | | |
|
| sag_25_30 | GAACAAATGGCACTAGTAA ACTGAG | AGTTTACTTTAAAAATTTG TTAAAAUUUAGCC | 1 | 0 | 0 | 0 | 3 | 5 | 0.603 | | |
|
| haat_bs_p_fw | AAACATGCCTAAACGCTTC | GAAGCGTTTAGGCATGTTT | 1 | 1 | 0 | 0 | 0 | 0 | 0 | | |
|
| haat_bs_r10_12_aat_fw | AAACATGCCTAAACGCTTC | GAAGCGTAATGGCATGTTT | 1 | 0 | 1 | 3 | 2 | 5.5 | 0.799 | 0.63 | h1 promoter | 0.36
|
| haat_bs_r10_12_aat_fw | AAACATGCCTAAACGCTTC | GAAGCGTAATGGCATGTTT | 1 | 0 | 1 | 3 | 2 | 5.5 | 0.799 | 0.55 | u6 promoter | 0.4
|
| haat_bs_r10_12_agc_fw | AAACATGCCTAAACGCTTC | GAAGCGTAGCGGCATGTTT | 1 | 0 | 1 | 3 | 2 | 5.5 | 0.749 | 0.32 | h1 promoter | 0.66
|
| haat_bs_r10_12_agc_fw | AAACATGCCTAAACGCTTC | GAAGCGTAGCGGCATGTTT | 1 | 0 | 1 | 3 | 2 | 5.5 | 0.749 | 0.37 | u6 promoter | 0.54
|
| haat_bs_m10at_fw | AAACATGCCTAAACGCTTC | GAAGCGTTTTGGCATGTTT | 1 | 0 | 1 | 1 | 2 | 6.5 | 0.799 | 0.9 | h1 promoter | 0.13
|
| haat_bs_m10at_fw | AAACATGCCTAAACGCTTC | GAAGCGTTTTGGCATGTTT | 1 | 0 | 1 | 1 | 2 | 6.5 | 0.799 | 0.9 | u6 promoter | 0.11
|
| haat_bs_m10ac_fw | AAACATGCCTAAACGCTTC | GAAGCGTTTCGGCATGTTT | 1 | 0 | 1 | 1 | 2 | 6.5 | 0.773 | 0.78 | h1 promoter | 0.24
|
| haat_bs_m10ac_fw | AAACATGCCTAAACGCTTC | GAAGCGTTTCGGCATGTTT | 1 | 0 | 1 | 1 | 2 | 6.5 | 0.773 | 0.77 | u6 promoter | 0.21
|
| haat_bs_m11ta_fw | AAACATGCCTAAACGCTTC | GAAGCGTTAAGGCATGTTT | 1 | 0 | 1 | 1 | 2 | 1.5 | 0.38 | 0.69 | h1 promoter | 0.32
|
| haat_bs_m11ta_fw | AAACATGCCTAAACGCTTC | GAAGCGTTAAGGCATGTTT | 1 | 0 | 1 | 1 | 2 | 1.5 | 0.38 | 0.74 | u6 promoter | 0.24
|
| haat_bs_m11tg_fw | AAACATGCCTAAACGCTTC | GAAGCGTTGAGGCATGTTT | 1 | 0 | 1 | 1 | 2 | 1.5 | 0.38 | 0.64 | h1 promoter | 0.37
|
| haat_bs_m11tg_fw | AAACATGCCTAAACGCTTC | GAAGCGTTGAGGCATGTTT | 1 | 0 | 1 | 1 | 2 | 1.5 | 0.38 | 0.66 | u6 promoter | 0.31
|
| haat_bs_r9_12_aatc_fw | AAACATGCCTAAACGCTTC | GAAGCGTAATCGCATGTTT | 1 | 0 | 1 | 4 | 2 | 1.5 | 0.38 | 0.27 | h1 promoter | 0.7
|
| haat_bs_r9_12_aatc_fw | AAACATGCCTAAACGCTTC | GAAGCGTAATCGCATGTTT | 1 | 0 | 1 | 4 | 2 | 1.5 | 0.38 | 0.48 | u6 promoter | 0.45
|
| haat_bs_r9_12_agcc_fw | AAACATGCCTAAACGCTTC | GAAGCGTAGCCGCATGTTT | 1 | 0 | 1 | 4 | 2 | 1.5 | 0.38 | 0.04 | h1 promoter | 0.92
|
| haat_bs_r9_12_agcc_fw | AAACATGCCTAAACGCTTC | GAAGCGTAGCCGCATGTTT | 1 | 0 | 1 | 4 | 2 | 1.5 | 0.38 | 0.04 | u6 promoter | 0.8
|
| haat_bs_onlyseed_fw | AAACATGCCTAAACGCTTC | CTTCGCAAATCGCATGTTT | 1 | 0 | 1 | 0 | 2 | 2 | 0.799 | 0.15 | h1 promoter | 0.81
|
| haat_bs_onlyseed_fw | AAACATGCCTAAACGCTTC | CTTCGCAAATCGCATGTTT | 1 | 0 | 1 | 0 | 2 | 2 | 0.799 | 0 | u6 promoter | 0.96
|
| sag_25_perfect_BS | GAACAAATGGCACTAGTAA ACTGAG | CTCAGTTTACTAGTGCCAT TTGTTC | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | miMeasure | 0.49
|
| sag_25_10-12_ACG | GAACAAATGGCACTAGTAA ACTGAG | CTCAGTTTACTAGACGCAT TTGTTC | 1 | 0 | 1 | 3 | 2 | 7.5 | 0.288 | 0.48 | miMeasure | 1.02
|
| sag_25_11-12_AA | GAACAAATGGCACTAGTAA ACTGAG | CTCAGTTTACTAGTAACAT TTGTTC | 1 | 0 | 1 | 2 | 2 | 8 | 0.33 | 0.47 | miMeasure | 0.98
|
| sag_25_9-12_ACGG | GAACAAATGGCACTAGTAA ACTGAG | CTCAGTTTACTAGACGGAT TTGTTC | 1 | 0 | 1 | 4 | 2 | 7.5 | 0.288 | 0.5 | miMeasure | 1.03
|
| sag_25_9-12_ATGT | GAACAAATGGCACTAGTAA ACTGAG | CTCAGTTTACTAGATGTAT TTGTTC | 1 | 0 | 1 | 4 | 2 | 7.5 | 0.332 | 0.47 | miMeasure | 1.06
|
| sag_25_ 10_G | GAACAAATGGCACTAGTAA ACTGAG | CTCAGTTTACTAGTGGCAT TTGTTC | 1 | 0 | 1 | 1 | 2 | 8.5 | 0.288 | 0.46 | miMeasure | 0.99
|
| sag_25_10_A | GAACAAATGGCACTAGTAA ACTGAG | CTCAGTTTACTAGTGACAT TTGTTC | 1 | 0 | 1 | 1 | 2 | 8.5 | 0.31 | 0.49 | miMeasure | 0.59
|
| sag_25_11_A | GAACAAATGGCACTAGTAA ACTGAG | CTCAGTTTACTAGTACCAT TTGTTC | 1 | 0 | 1 | 1 | 2 | 8 | 0.308 | 0.83 | miMeasure | 0.91
|
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