Team:Heidelberg/Notebook/BSDesign/October

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Binding Site Design - October

10/02/2010

digestion in 50 µl of
- 4x pSB1C3 (5µl BSA, 5µl NEB buffer 2, 1µl PstI, 1µl EcoRI, 1µl DpnI, 4µl construct, 33 µl H2O)
- 4x pSB1A3 (5µl BSA, 5µl NEB buffer 2, 1µl PstI, 1µl EcoRI, 1µl DpnI, 4µl construct, 33 µl H2O)

10/05/2010

1µg digestion of miMeasure and 6 different constructs (1A, 3F, 13, 15, 17, 37) with EcoRI and PstI

1= 0S 2= 0L 3= 10S 4= 10L

20 Miniprep:

**Table 1: 20 Minis**
Number	binding site against miRNA x	Position
1	221	3.5
2	221	1.4
3	1179	3.1
4	4286	2.6
5	4286	4.1
6	4286	3.4
7	1179	4.4
8	3.5
9	1179	1.9
10	221	4.6
11	221	1.5
12	4286	3.5
13	221	4.8
14	221	2.3
15	4286	2.2
16	4286	1.3
17	1179	2.5
18	1179	2.4
19	1179	1.5
20	1179	4.8

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