Don't take your E. coli everywhere - they might smell bad.
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Don't put your E. coli on your sophisticated laser apparatus - you might regret it and it might smell bad.
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Don't put your E. coli to close to your Millipore ion exchanger - the water quality might suffer.
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Don't vortex your E. coli cells too hard - they might puke and this is not nice...
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Don't spoil your E. coli cells everwhere - keep your stuff clean!
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If you put your E. coli cells into a fluorimeter and try to repeat our in vivo measurements and then wonder why they don't work - well may be crossed the OD600 of 0.05.
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If you don't understand why you only measure noise and ask your E. coli - most likely it won't tell you anything new...
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Colony PCR!
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Again, don't spill everywhere.
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In the end of the day, enjoy your beer. Don't let E. coli get into your way...
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