Week of 8/2

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Contents

August 2nd

  • Miniprep
- R0061
- R1062
- J23100 


  • Digests
- K091146 (SP)
- R1062 (SP)
- J23100 (SP)
- K091107 (SP)
- Lux+RBS+A3 (XP+phosphatase)
- RFP+RBS (XP+phophatase)


  • Gel order:
- J23100
- R1062
- K091146
- R0063
- K091107
- K145150
- Ladder
8-2-10.jpg
  • Ligations
- J23100 + Lux+RBS+A3
- R1062 + RFP+RBS
- K091146 + RFP+RBS
- K091107 + RFP+RBS

August 3rd

  • Colony PCR
1)K091107+RFP+RBS A-C
2)R1062+RFP+RBS A-D
3)J23100+LuxR+RBS+A3 A-D


  • Digest
- R0063 (SP)
- K145150 (SP)
- K091146 (SP)
- RFP+RBS (XP+phosphatase)
- Violet pigment 1 (EP)
- Violet pigment 2 (EP)
- A3 (EP+phosphatase)
  • Digest for 6 hours:
- J24679+B1002 (B)
- J24679+B1002 (D)


  • Gel order:
- Violet 1
- Violet 2
- K145150 
- R0063
- K091146 
- Ladder
8-3-10.jpg

The last two should have been about 7000 base pairs but it was much lower so the pieces were not cut out. The first piece (K091146) was cut out.

  • Gel number # 2:
-Row 1
  - Ladder
  - J24679+B1002 (B)
  - J24679+B1002 (D)
Row 2
  - Ladder
  - 1A 
  - 1B
  - 1C
  - 2A
  - 2B
  - 2C
  - 2D
  - 3A
  - 3B
  - 3C
  - 3D
8-3-10 001 8-3-10 002.jpg
  • PCRs did not show up. But, the bands for J24679+B1002 (B) and (D) were extracted


August 4th

  • Miniprep:
- R0061
  • Digests
- R0061 (SP+phosphatase)
- R0062 (SP+phosphatase)
- R0063 (SP+phosphatase)
- K145150 (SP+phosphatase)
- K091146 (SP+phosphatase)
- RFP+RBS (XP)
- J23100 (SP+phosphatase)
- Lux+RBS (XP)
  • Grow Up for tomorrow:
- RFP+RBS
- K091107
- K091146
  • Sent to sequencing:
- K091146+RFP+RBS (A-D)
8-4-10.jpg

August 5th

  • Digests
- RFP (E)
- RFP (X)
- RFP (S)
- RFP (P)
- RFP (EP)
- RFP (XS)
- Lux promoters with RFP but used violet pigment as the backbone
  • Minipreps:
- K091146+RFP+RBS
- RFP+RBS
- K091107
- K091146

  • Ligations:
- R0061 + RFP+RBS
- R0063 + RFP+RBS
- K091146 + RFP+RBS
- K145150 + RFP+RBS
- K091107 + RFP+RBS
- R1062 + RFP+RBS
  • Colony PCR
- J23100+Lux+RBS (A-F)
  • Gel order:
- Ladder
- J23100+Lux+RBS A-F
- Ladder 2
- RFP (E)
- RFP (X)
- RFP (S)
- RFP (P)
- RFP (EP)
- RFP (XS)
8-5-10.jpg

August 6th

  • Miniprep
-RFP+RBS

  • Gel order of PCR products:
- Ladder        - Ladder 2
- 1A            - 4B
- 1B            - 4C
- 1C            - 4D 
- 1D            - 5A
- 2A            - 5B
- 2B            - 5C
- 2C            - 6A
- 2D            - 6B
- 3A            - 6C
- 3B 
- 3C
- 3D
- 4A


  • Colony PCR
- R0063+RFP (A-D)
- K091107+RFP (A-D)
- K145150+RFP (A-D)
- R0061+RFP (A-D)
- K091146+RFP (A-C)
- R1062+RFP (A-C)
8-6-10.jpg
  • Today, we compared our enzymes to Mike Speer's (grad student) enzymes because we aren't sure if they are working correctly.
  • Sent to sequencing:
1)K091107+RFP (a)
2)R0061+RFP (a)


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