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Week of 8/2
From 2010.igem.org
Contents |
August 2nd
- Miniprep
- R0061 - R1062 - J23100
- Digests
- K091146 (SP) - R1062 (SP) - J23100 (SP) - K091107 (SP) - Lux+RBS+A3 (XP+phosphatase) - RFP+RBS (XP+phophatase)
- Gel order:
- J23100 - R1062 - K091146 - R0063 - K091107 - K145150 - Ladder
- Ligations
- J23100 + Lux+RBS+A3 - R1062 + RFP+RBS - K091146 + RFP+RBS - K091107 + RFP+RBS
August 3rd
- Colony PCR
1)K091107+RFP+RBS A-C 2)R1062+RFP+RBS A-D 3)J23100+LuxR+RBS+A3 A-D
- Digest
- R0063 (SP) - K145150 (SP) - K091146 (SP) - RFP+RBS (XP+phosphatase) - Violet pigment 1 (EP) - Violet pigment 2 (EP) - A3 (EP+phosphatase)
- Digest for 6 hours:
- J24679+B1002 (B) - J24679+B1002 (D)
- Gel order:
- Violet 1 - Violet 2 - K145150 - R0063 - K091146 - Ladder
The last two should have been about 7000 base pairs but it was much lower so the pieces were not cut out. The first piece (K091146) was cut out.
- Gel number # 2:
-Row 1 - Ladder - J24679+B1002 (B) - J24679+B1002 (D) Row 2 - Ladder - 1A - 1B - 1C - 2A - 2B - 2C - 2D - 3A - 3B - 3C - 3D
- PCRs did not show up. But, the bands for J24679+B1002 (B) and (D) were extracted
August 4th
- Miniprep:
- R0061
- Digests
- R0061 (SP+phosphatase) - R0062 (SP+phosphatase) - R0063 (SP+phosphatase) - K145150 (SP+phosphatase) - K091146 (SP+phosphatase) - RFP+RBS (XP) - J23100 (SP+phosphatase) - Lux+RBS (XP)
- Grow Up for tomorrow:
- RFP+RBS - K091107 - K091146
- Sent to sequencing:
- K091146+RFP+RBS (A-D)
August 5th
- Digests
- RFP (E) - RFP (X) - RFP (S) - RFP (P) - RFP (EP) - RFP (XS) - Lux promoters with RFP but used violet pigment as the backbone
- Minipreps:
- K091146+RFP+RBS - RFP+RBS - K091107 - K091146
- Ligations:
- R0061 + RFP+RBS - R0063 + RFP+RBS - K091146 + RFP+RBS - K145150 + RFP+RBS - K091107 + RFP+RBS - R1062 + RFP+RBS
- Colony PCR
- J23100+Lux+RBS (A-F)
- Gel order:
- Ladder - J23100+Lux+RBS A-F - Ladder 2 - RFP (E) - RFP (X) - RFP (S) - RFP (P) - RFP (EP) - RFP (XS)
August 6th
- Miniprep
-RFP+RBS
- Gel order of PCR products:
- Ladder - Ladder 2 - 1A - 4B - 1B - 4C - 1C - 4D - 1D - 5A - 2A - 5B - 2B - 5C - 2C - 6A - 2D - 6B - 3A - 6C - 3B - 3C - 3D - 4A
- Colony PCR
- R0063+RFP (A-D) - K091107+RFP (A-D) - K145150+RFP (A-D) - R0061+RFP (A-D) - K091146+RFP (A-C) - R1062+RFP (A-C)
- Today, we compared our enzymes to Mike Speer's (grad student) enzymes because we aren't sure if they are working correctly.
- Sent to sequencing:
1)K091107+RFP (a) 2)R0061+RFP (a)
