Week of 8/2

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August 2nd

Miniprep

- R0061
- R1062
- J23100

Digests

- K091146 (SP)
- R1062 (SP)
- J23100 (SP)
- K091107 (SP)
- Lux+RBS+A3 (XP+phosphatase)
- RFP+RBS (XP+phophatase)

Gel order:

- J23100
- R1062
- K091146
- R0063
- K091107
- K145150
- Ladder

Ligations

- J23100 + Lux+RBS+A3
- R1062 + RFP+RBS
- K091146 + RFP+RBS
- K091107 + RFP+RBS

August 3rd

Colony PCR

1)K091107+RFP+RBS A-C
2)R1062+RFP+RBS A-D
3)J23100+LuxR+RBS+A3 A-D

Digest

- R0063 (SP)
- K145150 (SP)
- K091146 (SP)
- RFP+RBS (XP+phosphatase)
- Violet pigment 1 (EP)
- Violet pigment 2 (EP)
- A3 (EP+phosphatase)

Digest for 6 hours:

- J24679+B1002 (B)
- J24679+B1002 (D)

Gel order:

- Violet 1
- Violet 2
- K145150 
- R0063
- K091146 
- Ladder

The last two should have been about 7000 base pairs but it was much lower so the pieces were not cut out. The first piece (K091146) was cut out.

Gel number # 2:

-Row 1
  - Ladder
  - J24679+B1002 (B)
  - J24679+B1002 (D)
Row 2
  - Ladder
  - 1A 
  - 1B
  - 1C
  - 2A
  - 2B
  - 2C
  - 2D
  - 3A
  - 3B
  - 3C
  - 3D

PCRs did not show up. But, the bands for J24679+B1002 (B) and (D) were extracted

August 4th

Miniprep:

- R0061

Digests

- R0061 (SP+phosphatase)
- R0062 (SP+phosphatase)
- R0063 (SP+phosphatase)
- K145150 (SP+phosphatase)
- K091146 (SP+phosphatase)
- RFP+RBS (XP)
- J23100 (SP+phosphatase)
- Lux+RBS (XP)

Grow Up for tomorrow:

- RFP+RBS
- K091107
- K091146

Sent to sequencing:

- K091146+RFP+RBS (A-D)

August 5th

Digests

- RFP (E)
- RFP (X)
- RFP (S)
- RFP (P)
- RFP (EP)
- RFP (XS)
- Lux promoters with RFP but used violet pigment as the backbone

Minipreps:

- K091146+RFP+RBS
- RFP+RBS
- K091107
- K091146

Ligations:

- R0061 + RFP+RBS
- R0063 + RFP+RBS
- K091146 + RFP+RBS
- K145150 + RFP+RBS
- K091107 + RFP+RBS
- R1062 + RFP+RBS

Colony PCR

- J23100+Lux+RBS (A-F)

Gel order:

- Ladder
- J23100+Lux+RBS A-F
- Ladder 2
- RFP (E)
- RFP (X)
- RFP (S)
- RFP (P)
- RFP (EP)
- RFP (XS)

August 6th

Miniprep

-RFP+RBS

Gel order of PCR products:

- Ladder        - Ladder 2
- 1A            - 4B
- 1B            - 4C
- 1C            - 4D 
- 1D            - 5A
- 2A            - 5B
- 2B            - 5C
- 2C            - 6A
- 2D            - 6B
- 3A            - 6C
- 3B 
- 3C
- 3D
- 4A

Colony PCR

- R0063+RFP (A-D)
- K091107+RFP (A-D)
- K145150+RFP (A-D)
- R0061+RFP (A-D)
- K091146+RFP (A-C)
- R1062+RFP (A-C)

Today, we compared our enzymes to Mike Speer's (grad student) enzymes because we aren't sure if they are working correctly.

Sent to sequencing:

1)K091107+RFP (a)
2)R0061+RFP (a)

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