Team:UCL London/Our BioBricks

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UCL IGEM 2010

RETURN TO IGEM 2010

OUR BIOBRICKS

The following table is that of all the Biobricks and parts that we have used over the course of iGem 2010. This does not necessary mean that they were all incorporated into the final product!

NEW PARTS SUBMITTED & CHARACTERISED

Part Name Part Type Description Designer Length(bp)
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K301000 BBa_K301000] Regulatory Tac promoter, hybrid derived from trp and lac promoters Xiang Chen 73
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K301001 BBa_K301001] Device pTAC with RFP reporter Xiang Chen 942


UCL-Constructing-Part-BBa-K.png










Check out our parts page to see the parts submitted in more detail [http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2010&group=UCL_London&Done=1|UCL_London 2010 iGEM Team Parts].

EXISTING PARTS/DEVICES CHARACTERISED

Part Code Part Name Location Backbone Length(bp)
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K239011 BBa_K239011] Stress Light GFP Anaerobic Metabolism Detector 2 972
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K239009 BBa_K239009] GFP test devise for Spy promoter 1045
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K239015 BBa_K239015] GFP test devise for DegP promoter 1116

OTHER PARTS/DEVICES TRIED/USED

Part Code Part Name Location Backbone Length(bp)
[http://partsregistry.org/wiki/index.php?title=Part:BBa_R0010 BBa_R0010] PROMOTER (lacI Regulated) Plate1_1D pSB1A2 200
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K239005 BBa_K239005] Nark Eurogentec pUC57 139
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K239006 BBa_K239006] mNark Eurogentec pUC57 89
[http://partsregistry.org/wiki/index.php?title=Part:BBa_R0079 BBa_R0079] Promoter (LasR & PAI regulated) Plate1_19P pSB1A2 936
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K274100 BBa_K274100] VRDP Plate3_6H pSB1A2 3385
[http://partsregistry.org/wiki/index.php?title=Part:BBa_B0015 BBa_B0015] Double Terminator Plate1_23L pSB1AK3 129
[http://partsregistry.org/wiki/index.php?title=pSB1K3 pSB1K3] Kanamycin Resistance Backbone Plate1_5A pSB1K3+J04450 insert 2206
[http://partsregistry.org/wiki/index.php?title=pSB1T3 pSB1T3] Tetracycline Resistance Backbone Plate 1_7A pSB1T3+J04450 insert 2463
[http://partsregistry.org/wiki/index.php?title=pSB1C3 pSB1C3] Chloramphenicol Resistance Backbone Plate 1_3A pSB1C3+J04450 insert 2072
[http://partsregistry.org/wiki/index.php?title=Part:BBa_R0011 BBa_R0011] Promoter (lacI regulated, lambda pL hybrid) Plate1_6G pSB1A2 55
[http://partsregistry.org/wiki/index.php?title=Part:BBa_B0034 BBa_B0034] RBS Plate1_2M pSB1A2 12
[http://partsregistry.org/wiki/index.php?title=Part:BBa_C0079 BBa_C0079] lasR activator from P. aeruginosa PAO1(+LVA) Plate1_14J pSB2K3 756
[http://partsregistry.org/wiki/index.php?title=pSB1AK3 pSB1AK3] Amp+Kana Plate1_11A +J04450 insert 3189
[http://partsregistry.org/wiki/index.php?title=pSB1AT3 pSB1AT3] Amp+Tetra Plate1_13A +J04450 insert 3446
[http://partsregistry.org/wiki/index.php?title=pSB1AC3 pSB1AC3] Amp+Chlora Plate1_9A +J04450 insert 3055
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K274200 BBa_K274200] CrtEBIY with rbs (Orange) Plate3_6L pSB1A2 4555
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K274002 BBa_K274002] Purple Plate3_12B pSB1T3 7345
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K274003 BBa_K274003] Dark Green Plate3_20H pSB1K3 6032
Plate3_20D pSB1T3 6032
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K274004 BBa_K274004] Light Green Plate3_20J pSB1K3 6200
Plate3_22F pSBT3 6200


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