Team:UCL London/Our BioBricks

From 2010.igem.org

UCL IGEM 2010

RETURN TO IGEM 2010

OUR BIOBRICKS

The following table is that of all the Biobricks and parts that we have used over the course of iGem 2010. Also, it's worth mentioning that we are the very first iGEM team to ever have 1.2 kg of biomass!!

NEW PARTS SUBMITTED & CHARACTERISED

Part Name Part Type Description Designer Length(bp)
BBa_K301000 Regulatory Tac promoter, hybrid derived from trp and lac promoters Xiang Chen 73
BBa_K301001 Device pTAC with RFP reporter Xiang Chen 942
BBa_K301003 Regulatory T7 promoter Xiang Chen 23
BBa_K301005 Coding T7 RNA Polymerase Xiang Chen 1000
BBa_K301006 Device Low Oxygen Level Self-induction Genetic Circuit. NarK promoter Xiang Chen 3082
BBa_K301007 Device Low Oxygen Level Self-induction Genetic Circuit. mNarK promoter Xiang Chen 3032
BBa_K301008 Device Self-induction Genetic Circuit with an Inducible Promoter Xiang Chen 3016
BBa_K301010 Composite Self-induction Genetic Circuit Xiang Chen 2937

Check out our parts page to see the parts submitted in more detail UCL_London 2010 iGEM Team Parts.

EXISTING PARTS/DEVICES CHARACTERISED

Part Code Part Name Location Backbone Length(bp)
BBa_K239011 Stress Light GFP Anaerobic Metabolism Detector 2 972
BBa_K239009 GFP test devise for Spy promoter 1045
BBa_K239015 GFP test devise for DegP promoter 1116

OTHER PARTS/DEVICES TRIED/USED

Part Code Part Name Location Backbone Length(bp)
BBa_R0010 PROMOTER (lacI Regulated) Plate1_1D pSB1A2 200
BBa_K239005 Nark Eurogentec pUC57 139
BBa_K239006 mNark Eurogentec pUC57 89
BBa_R0079 Promoter (LasR & PAI regulated) Plate1_19P pSB1A2 936
BBa_K274100 VRDP Plate3_6H pSB1A2 3385
BBa_B0015 Double Terminator Plate1_23L pSB1AK3 129
pSB1K3 Kanamycin Resistance Backbone Plate1_5A pSB1K3+J04450 insert 2206
pSB1T3 Tetracycline Resistance Backbone Plate 1_7A pSB1T3+J04450 insert 2463
pSB1C3 Chloramphenicol Resistance Backbone Plate 1_3A pSB1C3+J04450 insert 2072
BBa_R0011 Promoter (lacI regulated, lambda pL hybrid) Plate1_6G pSB1A2 55
BBa_B0034 RBS Plate1_2M pSB1A2 12
BBa_C0079 lasR activator from P. aeruginosa PAO1(+LVA) Plate1_14J pSB2K3 756
pSB1AK3 Amp+Kana Plate1_11A +J04450 insert 3189
pSB1AT3 Amp+Tetra Plate1_13A +J04450 insert 3446
pSB1AC3 Amp+Chlora Plate1_9A +J04450 insert 3055
BBa_K274200 CrtEBIY with rbs (Orange) Plate3_6L pSB1A2 4555
BBa_K274002 Purple Plate3_12B pSB1T3 7345
BBa_K274003 Dark Green Plate3_20H pSB1K3 6032
Plate3_20D pSB1T3 6032
BBa_K274004 Light Green Plate3_20J pSB1K3 6200
Plate3_22F pSBT3 6200
BBa_I13507 RBS+RFP+Terminator Pate1_22O pSB1A2 861


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