Gel Electrophoresis of the restriction digest of parts that Himika ran on 07-09-2010

Friday July 9, 2010

Himika

Today I did a restriction digest of parts with Eco/Spe in buffer II that I constructed and the plasmid switch I did on July 6th. The parts that I digested were:

• 3 colonies of E1010 in AC plasmid
• 2 colonies of E1010-B0015 construction done on July 6th
• I also ran 2 colonies of I13507
• 1 colony of E1010 in its original Kanamycin plasmid

Refer to the image for the results of the gel electrophoresis done of the restriction digests

I also updated the team and Dr. Schryvers about the IbpAB protein and modelling that I did this and last week.

I also designed primer along with Dave for the ppRFP circuit.