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Contents 1 yneA 1.1 Aims 1.2 Materials and protocols 1.3 Results 1.4 Conclusion

yneA

Aims

The aim of the experiment is to test if the filamentous cell part was cloned into pGFP-rrnB successfully.

Materials and protocols

1. Please refer to: Plasmid extraction.
2. Please refer to:Restriction digest. We used Nhe1 and Spe1 to remove the yneA from pGFP-rrnB.
3. Please refer to: Gel electrophoresis for running all the digested plasmid fragments.

Results

Figure 1: Gel electrophoresis result for restriction digest of pGFPrrnB and yneA with Nhe1 and Spe1.

• Lane 1: 1kb DNA ladder
• Lane 2: Tube 1
• Lane 3: Tube 2
• Lane 4: Tube 3
• Lane 5: Tube 4
• Lane 6: Tube 5
• Lane 7: Tube 6
• Lane 8: Tube 7
• Lane 9: Tube 8
• Lane 10: Tube 9
• Lane 11: Tube 10
• Lane 12: Tube 11
• Lane 13: Tube 12
• Lane 14: 1kb DNA ladder

Conclusion

The results show that the digest works, there is a band at approximately 541bp corresponding to yneA and a band at approximately 8.4kbp corresponding to pGFPrrnb in lanes 2, 3, 4, 6, 7, 8, 9, 11 and 12.