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Polymerase Chain Reaction

Aim

To amplify a desired DNA fragment by using a set of appropriate primers flanking the fragment.

Materials and Protocol

Please refer to: PCR for materials required and protocol.

Image 1: Image of the gel showing PCR products.

• Lane 1: 1kb DNA ladder
• Lane 2: Sample 1
• Lane 3: Sample 2
• Lane 4: Sample 3
• Lane 5: Sample 4
• Lane 6: Sample 5
• Lane 7: Sample 6
• Lane 8: Sample 7
• Lane 9: Sample 8
• Lane 10: Sample 9
• Lane 11: Sample 10
• Lane 12: Sample 11
• Lane 13: Sample 12
• Lane 14: Sample 13
• Lane 15: Sample 14