ID | Content | Registry link | Purpose of the cassette
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F6 | luc2_sv40ter | [http://partsregistry.org/Part:BBa_K337015 BBa_K337015] | Luc2 reporter gene with terminator referring to SV40 promoter
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F15 | CMV_TetO2 | [http://partsregistry.org/Part:BBa_K337027 BBa_K337027] | CMV promoter under control of Tet Operator for regulation of gene expression
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R1 | Sv40(rc)/RSV(fw) | [http://partsregistry.org/Part:BBa_K337045 BBa_K337045] | bidirectional hybrid promoter
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R2 | Sv40(rc)/CMV(fw) | [http://partsregistry.org/Part:BBa_K337047 BBa_K337047]
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R3 | RSV(rc)/CMV(fw) | [http://partsregistry.org/Part:BBa_K337048 BBa_K337048]
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R4 | RSV(rc)/CMV_TetO2(fw) | [http://partsregistry.org/Part:BBa_K337050 BBa_K337050]
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R5 | BGH(rc)/shRNA10(rc) | [http://partsregistry.org/Part:BBa_K337051 BBa_K337051] | synthetic microRNA template part
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R13 | BGH(rc)/shRNA10(rc)/sv40(rc)/RSV(fw) | [http://partsregistry.org/Part:BBa_K337020 BBa_K337020] | tuning construct cloning into reporter plasmid backbone containing a binding site against synthetic shRNA-like miRNA
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R14 | BGH(rc)/shRNA10(rc)/sv40(rc)/CMV(fw) | [http://partsregistry.org/Part:BBa_K337021 BBa_K337021]
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R15 | BGH(rc)/shRNA10(rc)/RSV(rc)/CMV(fw) | [http://partsregistry.org/Part:BBa_K337022 BBa_K337022]
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R16 | BGH(rc)/shRNA10(rc)/RSV(rc)/CMV(fw)_TetO2(fw) | [http://partsregistry.org/Part:BBa_K337023 BBa_K337023]
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R17 | BGH(rc)/shRNA6(rc)/sv40(rc)/RSV(fw) | [http://partsregistry.org/Part:BBa_K337024 BBa_K337024]
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R18 | BGH(rc)/shRNA6(rc)/sv40(rc)/CMV(fw) | [http://partsregistry.org/Part:BBa_K337026 BBa_K337026]
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R19 | BGH(rc)/shRNA6(rc)/RSV(rc)/CMV(fw) | [http://partsregistry.org/Part:BBa_K337029 BBa_K337029]
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R20 | BGH(rc)/shRNA6(rc)/RSV(rc)/CMV_TetO2(fw) | [http://partsregistry.org/Part:BBa_K337031 BBa_K337031] | tuning construct core containing Tet Operator for On-Targeting together with repressor construct
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R21 | Luc2(rc)_sv40(rc)/CMV(fw) | [http://partsregistry.org/Part:BBa_K337033 BBa_K337033] | bidirectional hybrid promoter with Luc2 reference gene under control of SV40 promoter
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R22 | Luc2(rc)_sv40(rc)/RSV(fw) | [http://partsregistry.org/Part:BBa_K337034 BBa_K337034]
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R32 | Kozag_hRluc/BGH(fw) | [http://partsregistry.org/Part:BBa_K337037 BBa_K337037] | reference reporter
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R33 | TetR_mut(XhoI/XbaI) | [http://partsregistry.org/Part:BBa_K337039 BBa_K337039] | mutagenized TetR including cutting sites to paste miRNA binding sites
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T1 | BGH(rc)_CMV/TetR/BGH(fw)/BGH(rc) | [http://partsregistry.org/Part:BBa_K337041 BBa_K337041] | repressor construct for On-Targeting together with tuning construct
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T2 | BGH(rc)_RSV/TetR/BGH(fw)/BGH(rc) | [http://partsregistry.org/Part:BBa_K337043 BBa_K337043]
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