araC promoter purification

Electrophoresed for gel extraction

Compared to marker band was a little lower than it should. Thinking that this was due to too big an amount, gel extracted anyway.

• Used TSUDA marker
• Part length is 1259 bp

Electrophoresed after gel extraction

Electrophoresis after purification

• Electrophoresed TSUDA I 2 uL and 0.5 uL of purified solution

→Estimated concentration to be 54 ng/uL

• This time band location is good
• Accidentally excised a part of other band resulting small contamination

Gel purification of GFP(1-12O) and concentration check

Electrophoresis for concentration check

• TSUDA I 2 uL, DNA 0.5 uL

→Estimated concentration to be 120 ng/uL

• Part length is 878 + 220 bp = 947 bp
• That slightly above mark so OK．