Team:Chiba/lux promoter

From 2010.igem.org

    We've charaterized Plux inverter.
    There is promoter (HSL-luxR repressor), biobrick number R0061.
    We've made Plux inv-GFP and characterized about it.

     Experiments
    

    1,Preparing Plux inx-GFP   

    cloning process is shown in Fig. 1

Fig. 1 cloning process

     Results
    

Fig. 2 assay process

1) Egland.K.A, and Greenberg.E.P, Conversion of the Vibrio Fischeri Transcriptional Activator,LuxR, to a Repressor, J. Bacteriol., 182, P.805-811 (2000)
2) Cox.R.S.3rd, Surette.M.G, Elowitz.M.B, Programming gene expression with combinatorial promoters, Mol Syst Biol, 3 ,145 (2007)