Team:Alberta/Notebook/ReusablePlates

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TEAM ALBERTA

Creating Inexpensive and Reusable Plates

(Abandoned)

The equipment and supplies required to sterilize and make plates are expensive and not usually available to a high school lab. We attempted to create plates which could easily be sterilized by a microwave or bleach.

Scintered Plastic Plates

Scintered Plastic Supplier: SPC technologies Ltd.

We used 3 tyes of scintered plastic:

  • 3.0mm Ultra-Fine PE sheet(14um pore-size)
    • ultra-fine grade should be a bacterial barrier
  • 6.0mm Fine-Grade PE Sheet(30um pore-size)
    • fine grade should also be a bacterial barrier but it was too hard to cut
  • 4.5mm Medium Grade PE Sheet(88um pore-size)

Procedure:

  1. tested plastic's ability to absorb LB
  • June 22, 2010 added 10ml of LB and 40ul chloramphenicol to plates made from the 3.0mm and 4.5mm scintered plastics. Incubated in a 37C warm room overnight.
  • June 23, 2010 7ml LB left in 3.0mm ultra-fine and 8ml left in 4.5mm medium-grade


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