Team:Queens-Canada/18 August 2010

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August 18, 2010

PCR

Yuli

pGpd-2

Program run using 9:1 FAST:HIFI blend with an extension time of 3 s and annealing temperature of 57 °C.

Digestion

Yuli and Hao

unc-54 3'UTR, pCeh-12, 7::eCFP, pSip-1::eGFP, pGpd-2, ChR2::eYFP, 3

unc-54 3'UTR, pGpd-2, and 1C3 cut with XbaI and PstI. All others cut with EcoRI and PstI.

unc-54 3'UTR::1C3, pCeh-12::1C3, NpHR::eCFP::1C3, pGpd-2::1C3, pSip-1::eGFP::1T3, pSB1C3, pSB1T3, eCFP::unc-54 3'UTR::1T3, ChR2::eYFP::unc-54 3'UTR::1T3

Liquid Culture

Yuli and Hao

eYFP, pSra-10, pMec-7, pHsp-3

PCR

Thai

pSrb-6

Program run using 9:1 FAST:HIFI blend with an extension time of 48 s and annealing temperature of 59 °C.

Gel Electrophoresis