Team:Queens-Canada/6 July 2010

PCR

Thai

pStr-1

Program run using HIFI with an extension time of 70 s and annealing temperature of 55 °C.

PCR

Hao

pFlp-1, pGpd-2

Gel Electrophoresis

Thai and Yuli

pOsm-10, pStr-220, pStr-1

Used 0.4 g agarose in 60 mL TBE. Gel run for 40 mins at 100 V.

Gel Electrophoresis

Yuli and Chris

pFlp-1, pGpd-2

Used 0.4 g agarose in 60 mL TBE. Gel run for 45 mins at 100 V.

PCR

Hao and Louis and Mary

pSra-10, pCeh-12

Program run using TAQ with an extension time of 125 s and annealing temperature of 59 °C.

Gel Electrophoresis

Hao and Louis and Mary

pStr-220

Used 1 g agarose in 50 mL TBE. Gel run for 20 mins at 120 V.