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Team:Queens-Canada/6 July 2010
From 2010.igem.org
July 6, 2010
PCR
Thai
pStr-1
Program run using HIFI with an extension time of 70 s and annealing temperature of 55 °C.
PCR
Hao
pFlp-1, pGpd-2
Gel Electrophoresis
Thai and Yuli
pOsm-10, pStr-220, pStr-1
Used 0.4 g agarose in 60 mL TBE. Gel run for 40 mins at 100 V.
Gel Electrophoresis
Yuli and Chris
pFlp-1, pGpd-2
Used 0.4 g agarose in 60 mL TBE. Gel run for 45 mins at 100 V.
PCR
Hao and Louis and Mary
pSra-10, pCeh-12
Program run using TAQ with an extension time of 125 s and annealing temperature of 59 °C.
Gel Electrophoresis
Hao and Louis and Mary
pStr-220
Used 1 g agarose in 50 mL TBE. Gel run for 20 mins at 120 V.
