Team:Newcastle/Meetings/26 March 2010
From 2010.igem.org
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Minutes for formal meeting 26th March 2010 |
* Room: CBCB; Start: 11 am |
* Chair: Jannetta, Minutes: Rachel, Computer: Harsh |
1. Roll call and apologies |
Jen, Janetta, Neil, Steven,Younus, Da, Alan, Richard, Harsh |
Phil – Holiday |
Apologies - Jem and Matt |
2. Approval of agenda and minutes |
Approved |
3. Brief summary of previous meeting |
Tutorial |
4. Research Feedback |
1. Properties of concrete and surface tension (Zoltan and Steven)
High compressive strength low tensile strength Steal added good for temp change Caco3 – similar to concrete; expand same as concrete... might crack like concrete Spider silk like steal- reduce possibility of cracking Glue- no many papers- expansion may not be a problem? Silk alkali resistant Capillary properties of concrete change over time- in project we consider small rapid Cement water ratio and surface area and environment Where to look for the cracks? Crack pattern |
2. Microinjection (Zoltan and Steven) |
Simple issue
Not correct term Swimming Bacillus Oxygen gradient big selling points |
3. Genetics Homologous recombination plasmid chromosome (Harsh and Rachel) |
Test in plasmids- Find good plasmid
Integrate into genome Recombination site... Find!!! amyE site amylase with insert can’t break down amylase |
4. Nutrients (Harsh and Rachel) |
Bacteria algae growth living in concrete
Only activate feed bacillus Two hybrid Bio films exclude other not algae Antifungals, anti algals, Antibiotic |
5. Sporulation, germination and secretion control (Alan and Da) |
SpoA – last years team sporulation control, maybe turn off completely
Nutrient availability KinA Inducible promoter |
Glue out of bug? |
6. Quorum sensing (Younus and Phil) |
Compx
And cff Two component system comp |
Why ? Don’t want?- not die after job fill new cracks
Nuclease Phil masEF Skf SbB If linear DNA works |
8. Glue, CaCo3 and spider silk (Zoltan and Jannetta)
Bacteria that produce CaCo3 from urea How secrete spider silk long complex proteins – how can we make it happen in bacillus Secretion systems |
9. Other points |
Linear DNA – Richard
Justifications of Linear Build it up the use. Mutations- How stop general questions Not specifically a problem – keep thinking about it though |
5. Action points |
All -Read Voygt paper |
All-Next week working off the proper wiki!!! |
Janetta contact details doc in drop box |
Janetta and Richard – Flow chart!!! |
Reminder to neil and jen not welcome trust funding by 2nd of April? |
Janetta- T-shirt sponsors and logo – embroidered / print mixed – 5 or 6 sponsors design your own- printing cheaper , quotes dependent on design |
Janetta- send us links for graphic design software- please |
Transfer the wiki |
1. Individual iGEM applications |
Phil hasn’t applied |
Richard not accepted |
Janetta text Phil |
Jen/Neil-Pay for team!! |
2. Visas/Passport update |
Harsh- May June apply |
Richard visa waiver |
3. Wiki transfer |
1. Clarify contents of identified pages |
2. Id Ethics page |
Research page |
Safety page |
entify other pages required |
3. Ideas for layout / theme |
Zoltan Tidy it up-Urgent |
Borrow last years / Look at anything thats nice |
It evolves!!! We can change it!!! Now!!!!!!! |
4. Travel plans |
1. Flights |
Need to sort out who is going |
2. Accomodation |
Need to sort out who is going |
5. Funding |
Harsh has made draft letter |
Main chemical company – free stuff ? |
6. Advisors/additional team members - civil engineer/graphics designer? |
Still looking –Neil,Jen |
7. Tutorials |
Sequentia and Clone manager- basics |
Modelling part two |
8. What next? |
Flow chart of what we’re going to do Big picture!!! |
If its coloured life is easier |
Cambridge brick-Colour |
Web services for Biosynthetics, Flow chart |
Deverner |
9. Time line discussion |
Need someone else on time line. |
16th of June Lab induction |
Deadlines soon |
Change flight deadline – not 31st |
6. Other business |
Pdf mark up tools – pdf exchange viewer, foxit, keeps mark up |
Making dropbox use better |
7. Items for next agenda |
Need wiki transferred by next meeting!!! |
Review of flow chart if done |
8. Next meeting- Thursday early as possible – email organise
1. Chair-Da 2. Minutes-Alan 3. Computer-Younus |