Team:Heidelberg/Notebook/Homology Based/August

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Homology Based Capsid Shuffling

31/08/2010

  • we decided to use AAV serotypes 1,2,5,6,8 and 9 as those have been described in the literature to infect pancreas cells.
  • cap plasmids (AAV 1, 2, 5, 6, 8, 9) have been diluted to a concentration of 200ng/ul
  • PCR on cap genes was performed using M13 primers as the genes are in the pBluescript vector:
    • AAV1 A
    • AAV2 A & B
    • AAV5 A & B
    • AAV6
    • AAV8 A & B
    • AAV9 A


August
MTWTFSS
1
2345678
9101112131415
16171819202122
23242526272829
3031

-


September
MTWTFSS
12345
6789101112
13141516171819
20212223242526
27282930

-

October
MTWTFSS
123
45 678910
11131415161718
19202122232425
2627282930

-


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