| mi/shRNA-name | sequence | BSsequence | number of BS
| perfect
| bulged
| bulge size
| seed type
| 3' score
| AU score
| knockdown [%]
| experimental conditions
|
| miR122_102 | TGGAGTGTGACAATGGTGTT- TGT | GACAAACACCATTGTCACAC- TCCA | 1 | 1 | 0 | 0 | 0 | 0 | 0 | |
|
| miR122_106 | TGGAGTGTGACAATGGTGTT- TGT | ACAAACACCATGAAGACACT- CCA | 1 | 0 | 1 | 4 | 3 | 7.5 | 0.624 | |
|
| miR122_134 | TGGAGTGTGACAATGGTGTT- TGT | ACAAACACCATACGGACACT- CCAGAGACACAAACACCA- TGAAGACACTCCA | 2 | 0 | 1 | 4 | 3 | 7.5 | 0.576 | |
|
| miR122_136 | TGGAGTGTGACAATGGTGTT- TGT | ACAAACACCATACGGACACT- CCA | 1 | 0 | 1 | 4 | 3 | 7.5 | 0.595 | |
|
| miR122_197 | TGGAGTGTGACAATGGTGTT- TGT | ACAAACACCATGTCGACACT- CCA | 1 | 0 | 1 | 4 | 3 | 7.5 | 0.597 | |
|
| miR122_199 | TGGAGTGTGACAATGGTGTT- TGT | ACAAACACCATGCCAACACT- CCA | 1 | 0 | 1 | 4 | 3 | 7.5 | 0.603 | |
|
| miR122_201 | TGGAGTGTGACAATGGTGTT- TGT | ACAAACACCATACGAACACT- CCA | 1 | 0 | 1 | 4 | 3 | 7.5 | 0.624 | |
|
| miR122_203 | TGGAGTGTGACAATGGTGTT- TGT | ACAAACACCATGCAGACACT- CCA | 1 | 0 | 1 | 4 | 3 | 7.5 | 0.6 | |
|
| miR122_277 | TGGAGTGTGACAATGGTGTT- TGT | ACAAACACCATGCCTACACT- CCA | 1 | 0 | 1 | 4 | 3 | 7.5 | 0.603 | |
|
| miR122_138 | TGGAGTGTGACAATGGTGTT- TGT | GGCCAGCACCATTTCACACA- CACTCCTTCTAGAGGCCGCT- GGC | 1 | 0 | 0 | 0 | 2 | 5 | 0.336 | |
|
| miR122_140 | TGGAGTGTGACAATGGTGTT- TGT | GCCCCTGATGGGGGCGACAC- TCCATCTAGAGGCCGCTGGC | 1 | 0 | 0 | 0 | 3 | 1.5 | 0.327 | |
|
| miR122_142 | TGGAGTGTGACAATGGTGTT- TGT | GACTAAGGCTGCTCCATCAA- CACTCCATCTAGAGGCCGCT- GGC | 1 | 0 | 0 | 0 | 3 | 4 | 0.314 | |
|
| miR122_144 | TGGAGTGTGACAATGGTGTT- TGT | GCAATGGAGAGTCACCTAGA- CACTCCATCTAGAGGCCGCT- GGC | 1 | 0 | 0 | 0 | 3 | 2.5 | 0.314 | |
|
| miR122_146 | TGGAGTGTGACAATGGTGTT- TGT | GACTTGAGCAGAACAAACAC- TCCATCTAGAGGCCGCTGGC | 1 | 0 | 0 | 0 | 3 | 2 | 0.327 | |
|
| miR122_148 | TGGAGTGTGACAATGGTGTT- TGT | GCAAATCATGATCAAAAACA- CTCCCTCTAGAGGCCGCTGG- C | 1 | 0 | 0 | 0 | 2 | 2.5 | 0.221 | |
|
| sAg_19_bs_r10_12_acg_fw | GAACAAATGGCACTAGTAA | TTACTAGACGCATTTGTTC | 1 | 0 | 1 | 3 | 2 | 5.5 | 0.442 | |
|
| sAg_19_bs_r10_12_taa_fw | GAACAAATGGCACTAGTAA | TTACTAGTAACATTTGTTC | 1 | 0 | 1 | 2 | 2 | 6 | 0.492 | |
|
| sAg_19_bs_r9_12_acgg_fw | GAACAAATGGCACTAGTAA | TTACTAGACGGATTTGTTC | 1 | 0 | 1 | 4 | 2 | 5.5 | 0.442 | |
|
| sAg_19_bs_r9_12_atgt_fw | GAACAAATGGCACTAGTAA | TTACTAGATGTATTTGTTC | 1 | 0 | 1 | 4 | 2 | 5.5 | 0.495 | |
|
| sAg_19_bs_m10cg_fw | GAACAAATGGCACTAGTAA | TTACTAGTGGCATTTGTTC | 1 | 0 | 1 | 1 | 2 | 6.5 | 0.442 | |
|
| sAg_19_bs_m10ca_fw | GAACAAATGGCACTAGTAA | TTACTAGTGACATTTGTTC | 1 | 0 | 1 | 1 | 2 | 6.5 | 0.496 | |
|
| sAg_19_bs_m11gc_fw | GAACAAATGGCACTAGTAA | TTACTAGTCCCATTTGTTC | 1 | 0 | 1 | 1 | 2 | 6 | 0.442 | |
|
| sAg_19_bs_m11ga_fw | GAACAAATGGCACTAGTAA | TTACTAGTACCATTTGTTC | 1 | 0 | 1 | 1 | 2 | 6 | 0.466 | |
|
| sAg_19_bs_onlyseed_fw_E | GAACAAATGGCACTAGTAA | AATGATCACGGATTTGTTC | 1 | 0 | 0 | 0 | 2 | 0 | 0.442 | |
|
| sAg_19_bs_p_fw_E | GAACAAATGGCACTAGTAA | TTACTAGTGCCATTTGTTC | 1 | 1 | 0 | 0 | 0 | 0 | 0 | |
|
| sag_25_1 | GAACAAATGGCACTAGTAAA- CTGAG | ATAATTTGTTCATTTGTTC | 2 | 0 | 0 | 0 | 2 | 1.5 | 0.491 | |
|
| sag_25_2 | GAACAAATGGCACTAGTAAA- CTGAG | ATAATTTGTTCATTTGTTCA- TTTGTTC | 3 | 0 | 0 | 0 | 2 | 1.5 | 0.491 | |
|
| sag_25_3 | GAACAAATGGCACTAGTAAA- CTGAG | AGTTTACTAGTGCCATTTGT- TCAAAUAUAGCC | 1 | 1 | 0 | 0 | 0 | 0 | 0 | |
|
| sag_25_4 | GAACAAATGGCACTAGTAAA- CTGAG | AGTTTACTAGTGCAATTTGT- TAAAAUUUAGCC | 1 | 0 | 1 | 1 | 3 | 8.5 | 0.587 | |
|
| sag_25_5 | GAACAAATGGCACTAGTAAA- CTGAG | AGTTTACTAGTGAAATTTGT- TAAAAUUUAGCC | 1 | 0 | 1 | 2 | 3 | 8 | 0.587 | |
|
| sag_25_6 | GAACAAATGGCACTAGTAAA- CTGAG | AGTTTACTAGTAAAATTTGT- TAAAAUUUAGCC | 1 | 0 | 1 | 3 | 3 | 7.5 | 0.587 | |
|
| sag_25_7 | GAACAAATGGCACTAGTAAA- CTGAG | AGTTTACTAGAAAAATTTGT- TAAAAUUUAGCC | 1 | 0 | 1 | 4 | 3 | 7 | 0.587 | |
|
| sag_25_8 | GAACAAATGGCACTAGTAAA- CTGAG | CTGGGCAATTATAAATTTGT- TAAAAUUUAGCC | 1 | 0 | 0 | 0 | 3 | 2 | 0.603 | |
|
| sag_25_9 | GAACAAATGGCACTAGTAAA- CTGAG | CTGGGCAGCCGCAAATTTGT- TAAAGGCCCGCC | 1 | 0 | 0 | 0 | 3 | 2 | 0.305 | |
|
| sag_25_10 | GAACAAATGGCACTAGTAAA- CTGAG | CTGGGCAGCTATAATTTTG- TTAAAAUUUAGCC | 1 | 0 | 0 | 0 | 1 | 2.5 | 0.69 | |
|
| sag_25_11 | GAACAAATGGCACTAGTAAA- CTGAG | AGTTTACGCCGTAAATTTG- TTGAAGGCCCGCC | 1 | 0 | 0 | 0 | 2 | 4 | 0.226 | |
|
| sag_25_12 | GAACAAATGGCACTAGTAAA- CTGAG | CTGGGCAATTATAAATTTG- TTGAAAUUUAGCC | 1 | 0 | 0 | 0 | 2 | 2 | 0.526 | |
|
| sag_25_13 | GAACAAATGGCACTAGTAAA- CTGAG | TCCTTACTAGTGCAATTTG- TTAAAGGCCCGCC | 1 | 0 | 0 | 0 | 3 | 7 | 0.305 | |
|
| sag_25_14 | GAACAAATGGCACTAGTAAA- CTGAG | CTGAATATAGTGAAATTTG- TTAAAAUUUAGCC | 1 | 0 | 0 | 0 | 3 | 4 | 0.603 | |
|
| sag_25_15 | GAACAAATGGCACTAGTAAA- CTGAG | AGTTTACTACCTAATTTTG- TTAAAAUCCGGCC | 1 | 0 | 0 | 0 | 1 | 4 | 0.497 | |
|
| sag_25_16 | GAACAAATGGCACTAGTAAA- CTGAG | CTGGGCCTAGTGGATTTTG- TTAAAGGCCCGCC | 1 | 0 | 0 | 0 | 1 | 5 | 0.366 | |
|
| sag_25_17 | GAACAAATGGCACTAGTAAA- CTGAG | AGTTTACATTGCAAATTTG- TTGAAGGCCCGCC | 1 | 0 | 0 | 0 | 2 | 4 | 0.226 | |
|
| sag_25_18 | GAACAAATGGCACTAGTAAA- CTGAG | CTGGGACTAGTGCAATTTG- TTGAAGGCCCGCC | 1 | 0 | 0 | 0 | 2 | 6 | 0.226 | |
|
| sag_25_20 | GAACAAATGGCACTAGTAAA- CTGAG | AGTTTACTAGAAAATTTTG- TTAAAAUUUAGCC | 1 | 0 | 0 | 0 | 1 | 7 | 0.69 | |
|
| sag_25_21 | GAACAAATGGCACTAGTAAA- CTGAG | AGTTTACTAGAAAAATTTG- TTGAAAUUUAGCC | 1 | 0 | 0 | 0 | 2 | 7 | 0.526 | |
|
| sag_25_23 | GAACAAATGGCACTAGTAAA- CTGAG | CTGGGCATAGATAATTTTG- TTAAAAUUUAGCC | 1 | 0 | 0 | 0 | 1 | 3 | 0.69 | |
|
| sag_25_24 | GAACAAATGGCACTAGTAAA- CTGAG | CTGGTACTAGCTAATTTTG- TTAAAAUCCGGCC | 1 | 0 | 0 | 0 | 1 | 5 | 0.497 | |
|
| sag_25_25 | GAACAAATGGCACTAGTAAA- CTGAG | AGTTTACTAGCCGATTTTG- TTAAAGGCCCGCC | 1 | 0 | 0 | 0 | 1 | 7 | 0.366 | |
|
| sag_25_26 | GAACAAATGGCACTAGTAAA- CTGAG | CTGGGCCTAGAAAAATTTG- TTGAAAUCCGGCC | 1 | 0 | 0 | 0 | 2 | 4 | 0.348 | |
|
| sag_25_27 | GAACAAATGGCACTAGTAAA- CTGAG | CTTTTACTAGAAAAATTTG- TTGAAUUUAGCC | 1 | 0 | 0 | 0 | 2 | 6 | 0.51 | |
|
| sag_25_28 | GAACAAATGGCACTAGTAAA- CTGAG | CTGGTACTAGGCAAATTTG- TTGAAGGCCCGCC | 1 | 0 | 0 | 0 | 2 | 5 | 0.226 | |
|
| sag_25_29 | GAACAAATGGCACTAGTAAA- CTGAG | GCTTTACTAGAAAAATTTG- TTAAAAUUUAGCC | 1 | 0 | 0 | 0 | 3 | 6 | 0.603 | |
|
| sag_25_30 | GAACAAATGGCACTAGTAAA- CTGAG | AGTTTACTTTAAAAATTTG- TTAAAAUUUAGCC | 1 | 0 | 0 | 0 | 3 | 5 | 0.603 | |
|
| haat_bs_p_fw | AAACATGCCTAAACGCTTC | GAAGCGTTTAGGCATGTTT | 1 | 1 | 0 | 0 | 0 | 0 | 0 | |
|
| haat_bs_r10_12_aat_fw | AAACATGCCTAAACGCTTC | GAAGCGTAATGGCATGTTT | 1 | 0 | 1 | 3 | 2 | 5.5 | 0.799 | |
|
| haat_bs_r10_12_agc_fw | AAACATGCCTAAACGCTTC | GAAGCGTAGCGGCATGTTT | 1 | 0 | 1 | 3 | 2 | 5.5 | 0.749 | |
|
| haat_bs_m10at_fw | AAACATGCCTAAACGCTTC | GAAGCGTTTTGGCATGTTT | 1 | 0 | 1 | 1 | 2 | 6.5 | 0.799 | |
|
| haat_bs_m10ac_fw | AAACATGCCTAAACGCTTC | GAAGCGTTTCGGCATGTTT | 1 | 0 | 1 | 1 | 2 | 6.5 | 0.773 | |
|
| haat_bs_m11ta_fw | AAACATGCCTAAACGCTTC | GAAGCGTTAAGGCATGTTT | 1 | 0 | 1 | 1 | 2 | 1.5 | 0.38 | |
|
| haat_bs_m11tg_fw | AAACATGCCTAAACGCTTC | GAAGCGTTGAGGCATGTTT | 1 | 0 | 1 | 1 | 2 | 1.5 | 0.38 | |
|
| haat_bs_r9_12_aatc_fw | AAACATGCCTAAACGCTTC | GAAGCGTAATCGCATGTTT | 1 | 0 | 1 | 4 | 2 | 1.5 | 0.38 | |
|
| haat_bs_r9_12_agcc_fw | AAACATGCCTAAACGCTTC | GAAGCGTAGCCGCATGTTT | 1 | 0 | 1 | 4 | 2 | 1.5 | 0.38 | |
|
| haat_bs_onlyseed_fw | AAACATGCCTAAACGCTTC | CTTCGCAAATCGCATGTTT | 1 | 0 | 1 | 0 | 2 | 2 | 0.799 | |
|
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