July, 22nd

Production Team

Paola Gallardo. Site-directed mutagenesis (SDM): First try with gltD** and fecI-fecR*, using an overlapping PCR reaction. We analyzed the products of the first round. Fail. We'll try again

David Caballero. Transformation of competent bacteria with ligation products we made the day before. We spread them in LB+Cm plates and let them grow overnight at 37ºC.

Assembly Team

UPO1+2 was analized by 1.5% agarose gel electrophoresis again. Results showed three bands with the same size, so our candidate is negative. We started again with UPO1+2: digesting, binding, transforming.