From 2010.igem.org
iGEM Panama
August 23
Today was a productive day.
We prepared 50 ml of Poliacrylamide gel 8% with TBE 0.5M.
- 13.3ml ABA (Acrylamide - Bisacrylamide)
- 2.5ml of TBE 10X
- 34.2ml of H2O
- 50ul of APS 25%
- 50ul of TEMED
We have to seal the edges with 2% of agarose so the poliacrylamide doesn't leak out. Then we have to fill the system with 50ml of TBE 0.5M and it is stained with 500ml of H2O and 25ul of Ethydium Bromide for 20 minutes.
We'll use 10-15ul of sample and it is set at 150V and 17 mamps.
12 % poliacrylamide gel
- 19.95ml ABA 10 ml
- 3.75ml TBE 10X 1,87 ml
- 75ulAPS 37,5 ul
- 75ul TEMED 37,5 ul
- 26.3ml H2O 13, 15 ul
After all of this, we finally, started the ligation of RBS, promoter and plasmid.
The ligation mix that we'll use:
So to get a final volume of 20ul, then we incubated this for 2 hours at room temperature and later at 80ºC for 20 minutes to deactivate the enzyme.
And good news for everyone, the ligation was done right! So now we have our RBS and promotor in a plasmid.
May
M T W T F S S
[http://2010.igem.org/wiki/index.php?title=Team:Panama/1_May_2010&action=edit 1 ]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/2_May_2010&action=edit 2 ]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/3_May_2010&action=edit 3 ]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/4_May_2010&action=edit 4 ]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/5_May_2010&action=edit 5 ]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/6_May_2010&action=edit 6 ]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/7_May_2010&action=edit 7 ]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/8_May_2010&action=edit 8 ]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/9_May_2010&action=edit 9 ]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/10_May_2010&action=edit 10 ]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/11_May_2010&action=edit 11 ]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/12_May_2010&action=edit 12 ]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/13_May_2010&action=edit 13 ]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/14_May_2010&action=edit 14 ]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/15_May_2010&action=edit 15 ]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/16_May_2010&action=edit 16 ]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/17_May_2010&action=edit 17 ]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/18_May_2010&action=edit 18 ]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/19_May_2010&action=edit 19 ]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/20_May_2010&action=edit 20 ]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/21_May_2010&action=edit 21 ]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/22_May_2010&action=edit 22 ]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/23_May_2010&action=edit 23 ]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/24_May_2010&action=edit 24 ]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/25_May_2010&action=edit 25 ]
[http://2010.igem.org/Team:Panama/26_May_2010 26 ]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/27_May_2010&action=edit 27 ]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/28_May_2010&action=edit 28 ]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/29_May_2010&action=edit 29 ]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/30_May_2010&action=edit 30 ]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/31_May_2010&action=edit 31 ]
June
M T W T F S S
[http://2010.igem.org/wiki/index.php?title=Team:Panama/1_June_2010&action=edit 1 ]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/2_June_2010&action=edit 2 ]
[http://2010.igem.org/Team:Panama/3_June_2010 3 ]
[http://2010.igem.org/Team:Panama/4_June_2010 4 ]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/5_June_2010&action=edit 5 ]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/6_June_2010&action=edit 6 ]
[http://2010.igem.org/Team:Panama/7_June_2010 7 ]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/8_June_2010&action=edit 8 ]
[http://2010.igem.org/Team:Panama/9_June_2010 9 ]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/10_June_2010&action=edit 10 ]
[http://2010.igem.org/Team:Panama/11_June_2010 11 ]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/12_June_2010&action=edit 12 ]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/13_June_2010&action=edit 13 ]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/14_June_2010&action=edit 14 ]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/15_June_2010&action=edit 15 ]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/16_June_2010&action=edit 16 ]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/17_June_2010&action=edit 17 ]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/18_June_2010&action=edit 18 ]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/19_June_2010&action=edit 19 ]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/20_June_2010&action=edit 20 ]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/21_June_2010&action=edit 21 ]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/22_June_2010&action=edit 22 ]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/23_June_2010&action=edit 23 ]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/24_June_2010&action=edit 24 ]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/25_June_2010&action=edit 25 ]
[http://2010.igem.org/Team:Panama/26_June_2010 26 ]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/27_June_2010&action=edit 27 ]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/28_June_2010&action=edit 28 ]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/29_June_2010&action=edit 29 ]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/30_June_2010&action=edit 30 ]
July
M T W T F S S
[http://2010.igem.org/wiki/index.php?title=Team:Panama/1_July_2010&action=edit 1 ]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/2_July_2010&action=edit 2 ]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/3_July_2010&action=edit 3 ]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/4_July_2010&action=edit 4 ]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/5_July_2010&action=edit 5 ]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/6_July_2010&action=edit 6 ]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/7_July_2010&action=edit 7 ]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/8_July_2010&action=edit 8 ]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/9_July_2010&action=edit 9 ]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/10_July_2010&action=edit 10 ]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/11_July_2010&action=edit 11 ]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/12_July_2010&action=edit 12 ]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/13_July_2010&action=edit 13 ]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/14_July_2010&action=edit 14 ]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/15_July_2010&action=edit 15 ]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/16_July_2010&action=edit 16 ]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/17_July_2010&action=edit 17 ]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/18_July_2010&action=edit 18 ]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/19_July_2010&action=edit 19 ]
[http://2010.igem.org/Team:Panama/20_July_2010 20 ]
[http://2010.igem.org/Team:Panama/21_July_2010 21 ]
[http://2010.igem.org/Team:Panama/22_July_2010 22 ]
[http://2010.igem.org/Team:Panama/23_July_2010 23 ]
[http://2010.igem.org/Team:Panama/24_July_2010 24 ]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/25_July_2010&action=edit 25 ]
[http://2010.igem.org/Team:Panama/26_July_2010 26 ]
[http://2010.igem.org/Team:Panama/27_July_2010 27 ]
[http://2010.igem.org/Team:Panama/28_July_2010 28 ]
[http://2010.igem.org/Team:Panama/29_July_2010 29 ]
[http://2010.igem.org/Team:Panama/30_July_2010 30 ]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/31_July_2010&action=edit 31 ]
August
M T W T F S S
[http://2010.igem.org/wiki/index.php?title=Team:Panama/1_August_2010&action=edit 1 ]
[http://2010.igem.org/Team:Panama/2_August_2010 2 ]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/3_August_2010&action=edit 3 ]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/4_August_2010&action=edit 4 ]
[http://2010.igem.org/Team:Panama/5_August_2010 5 ]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/6_August_2010&action=edit 6 ]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/7_August_2010&action=edit 7 ]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/8_August_2010&action=edit 8 ]
[http://2010.igem.org/Team:Panama/9_August_2010 9 ]
[http://2010.igem.org/Team:Panama/10_August_2010 10 ]
[http://2010.igem.org/Team:Panama/11_August_2010 11 ]
[http://2010.igem.org/Team:Panama/12_August_2010 12 ]
[http://2010.igem.org/Team:Panama/13_August_2010 13 ]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/14_August_2010&action=edit 14 ]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/15_August_2010&action=edit 15 ]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/16_August_2010&action=edit 16 ]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/17_August_2010&action=edit 17 ]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/18_August_2010&action=edit 18 ]
[http://2010.igem.org/Team:Panama/19_August_2010 19 ]
[http://2010.igem.org/Team:Panama/20_August_2010 20 ]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/21_August_2010&action=edit 21 ]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/22_August_2010&action=edit 22 ]
[http://2010.igem.org/Team:Panama/23_August_2010 23 ]
[http://2010.igem.org/Team:Panama/24_August_2010 24 ]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/25_August_2010&action=edit 25 ]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/26_August_2010&action=edit 26 ]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/27_August_2010&action=edit 27 ]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/28_August_2010&action=edit 28 ]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/29_August_2010&action=edit 29 ]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/30_August_2010&action=edit 30 ]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/31_August_2010&action=edit 31 ]
September
M T W T F S S
[http://2010.igem.org/wiki/index.php?title=Team:Panama/1_September_2010&action=edit 1 ]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/2_September_2010&action=edit 2 ]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/3_September_2010&action=edit 3 ]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/4_September_2010&action=edit 4 ]
[http://2010.igem.org/Team:Panama/5_September_2010 5 ]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/6_September_2010&action=edit 6 ]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/7_September_2010&action=edit 7 ]
[http://2010.igem.org/Team:Panama/8_September_2010 8 ]
[http://2010.igem.org/Team:Panama/9_September_2010 9 ]
[http://2010.igem.org/Team:Panama/10_September_2010 10 ]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/11_September_2010&action=edit 11 ]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/12_September_2010&action=edit 12 ]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/13_September_2010&action=edit 13 ]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/14_September_2010&action=edit 14 ]
[http://2010.igem.org/Team:Panama/15_September_2010 15 ]
[http://2010.igem.org/Team:Panama/16_September_2010 16 ]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/17_September_2010&action=edit 17 ]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/18_September_2010&action=edit 18 ]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/19_September_2010&action=edit 19 ]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/20_September_2010&action=edit 20 ]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/21_September_2010&action=edit 21 ]
[http://2010.igem.org/Team:Panama/22_September_2010 22 ]
[http://2010.igem.org/Team:Panama/23_September_2010 23 ]
[http://2010.igem.org/Team:Panama/24_September_2010 24 ]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/25_September_2010&action=edit 25 ]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/26_September_2010&action=edit 26 ]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/27_September_2010&action=edit 27 ]
[http://2010.igem.org/Team:Panama/28_September_2010 28 ]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/29_September_2010&action=edit 29 ]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/30_September_2010&action=edit 30 ]
October
M T W T F S S
[http://2010.igem.org/wiki/index.php?title=Team:Panama/1_October_2010&action=edit 1 ]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/2_October_2010&action=edit 2 ]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/3_October_2010&action=edit 3 ]
[http://2010.igem.org/Team:Panama/4_October_2010 4 ]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/5_October_2010&action=edit 5 ]
[http://2010.igem.org/Team:Panama/6_October_2010 6 ]
[http://2010.igem.org/Team:Panama/7_October_2010 7 ]
[http://2010.igem.org/Team:Panama/8_October_2010 8 ]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/9_October_2010&action=edit 9 ]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/10_October_2010&action=edit 10 ]
[http://2010.igem.org/Team:Panama/11_October_2010 11 ]
[http://2010.igem.org/Team:Panama/12_October_2010 12 ]
[http://2010.igem.org/Team:Panama/13_October_2010 13 ]
[http://2010.igem.org/Team:Panama/14_October_2010 14 ]
[http://2010.igem.org/Team:Panama/15_October_2010 15 ]
[http://2010.igem.org/Team:Panama/16_October_2010 16 ]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/17_October_2010&action=edit 17 ]
[http://2010.igem.org/Team:Panama/18_October_2010 18 ]
[http://2010.igem.org/Team:Panama/19_October_2010 19 ]
[http://2010.igem.org/Team:Panama/20_October_2010 20 ]
[http://2010.igem.org/Team:Panama/21_October_2010 21 ]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/22_October_2010&action=edit 22 ]
[http://2010.igem.org/Team:Panama/23_October_2010 23 ]
[http://2010.igem.org/Team:Panama/24_October_2010 24 ]
[http://2010.igem.org/Team:Panama/25_October_2010 25 ]
[http://2010.igem.org/Team:Panama/26_October_2010 26 ]
[http://2010.igem.org/Team:Panama/27_October_2010 27 ]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/28_October_2010&action=edit 28 ]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/29_October_2010&action=edit 29 ]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/30_October_2010&action=edit 30 ]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/31_October_2010&action=edit 31 ]