Notebook
June
|
M | T | W | T | F | S | S
|
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/1_June_2010&action=edit 1]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/2_June_2010&action=edit 2]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/3_June_2010&action=edit 3]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/4_June_2010&action=edit 4]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/5_June_2010&action=edit 5]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/6_June_2010&action=edit 6]
|
[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/7_June_2010&action=edit 7]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/8_June_2010&action=edit 8]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/9_June_2010&action=edit 9]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/10_June_2010&action=edit 10]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/11_June_2010&action=edit 11]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/12_June_2010&action=edit 12]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/13_June_2010&action=edit 13]
|
[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/14_June_2010&action=edit 14]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/15_June_2010&action=edit 15]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/16_June_2010&action=edit 16]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/17_June_2010&action=edit 17]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/18_June_2010&action=edit 18]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/19_June_2010&action=edit 19]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/20_June_2010&action=edit 20]
|
[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/21_June_2010&action=edit 21]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/22_June_2010&action=edit 22]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/23_June_2010&action=edit 23]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/24_June_2010&action=edit 24]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/25_June_2010&action=edit 25]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/26_June_2010&action=edit 26]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/27_June_2010&action=edit 27]
|
[http://2010.igem.org/Talk:Team:IvyTech-South_Bend/28_June_2010 28]
| [http://2010.igem.org/Talk:Team:IvyTech-South_Bend/29_June_2010 29]
| [http://2010.igem.org/Talk:Team:IvyTech-South_Bend/30_June_2010 30]
|
|
July
|
M | T | W | T | F | S | S
|
|
|
| [http://2010.igem.org/Talk:Team:IvyTech-South_Bend/1_July_2010 1]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/2_July_2010&action=edit 2]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/3_July_2010&action=edit 3]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/4_July_2010&action=edit 4]
|
[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/5_July_2010&action=edit 5]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/6_July_2010&action=edit 6]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/7_July_2010&action=edit 7]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/8_July_2010&action=edit 8]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/9_July_2010&action=edit 9]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/10_July_2010&action=edit 10]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/11_July_2010&action=edit 11]
|
[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/12_July_2010&action=edit 12]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/13_July_2010&action=edit 13]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/14_July_2010&action=edit 14]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/15_July_2010&action=edit 15]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/16_July_2010&action=edit 16]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/17_July_2010&action=edit 17]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/18_July_2010&action=edit 18]
|
[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/19_July_2010&action=edit 19]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/20_July_2010&action=edit 20]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/21_July_2010&action=edit 21]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/22_July_2010&action=edit 22]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/23_July_2010&action=edit 23]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/24_July_2010&action=edit 24]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/25_July_2010&action=edit 25]
|
[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/26_July_2010&action=edit 26]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/27_July_2010&action=edit 27]
| [http://2010.igem.org/Talk:Team:IvyTech-South_Bend/28_July_2010 28]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/29_July_2010&action=edit 29]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/30_July_2010&action=edit 30]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/31_July_2010&action=edit 31]
|
|
August
|
M | T | W | T | F | S | S
|
|
|
|
|
|
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/1_August_2010&action=edit 1]
|
[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/2_August_2010&action=edit 2]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/3_August_2010&action=edit 3]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/4_August_2010&action=edit 4]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/5_August_2010&action=edit 5]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/6_August_2010&action=edit 6]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/7_August_2010&action=edit 7]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/8_August_2010&action=edit 8]
|
[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/9_August_2010&action=edit 9]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/10_August_2010&action=edit 10]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/11_August_2010&action=edit 11]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/12_August_2010&action=edit 12]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/13_August_2010&action=edit 13]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/14_August_2010&action=edit 14]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/15_August_2010&action=edit 15]
|
[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/16_August_2010&action=edit 16]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/17_August_2010&action=edit 17]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/18_August_2010&action=edit 18]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/19_August_2010&action=edit 19]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/20_August_2010&action=edit 20]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/21_August_2010&action=edit 21]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/22_August_2010&action=edit 22]
|
[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/23_August_2010&action=edit 23]
| [http://2010.igem.org/Talk:Team:IvyTech-South_Bend/24_August_2010 24]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/25_August_2010&action=edit 25]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/26_August_2010&action=edit 26]
| [http://2010.igem.org/Talk:Team:IvyTech-South_Bend/27_August_2010 27]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/28_August_2010&action=edit 28]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/29_August_2010&action=edit 29]
|
[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/30_August_2010&action=edit 30]
| [http://2010.igem.org/Talk:Team:IvyTech-South_Bend/31_August_2010 31]
|
|
September
|
M | T | W | T | F | S | S
|
|
| [http://2010.igem.org/Talk:Team:IvyTech-South_Bend/1_September_2010 1]
| [http://2010.igem.org/Talk:Team:IvyTech-South_Bend/2_September_2010 2]
| [http://2010.igem.org/Talk:Team:IvyTech-South_Bend/3_September_2010 3]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/4_September_2010&action=edit 4]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/5_September_2010&action=edit 5]
|
[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/6_September_2010&action=edit 6]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/7_September_2010&action=edit 7]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/8_September_2010&action=edit 8]
| [http://2010.igem.org/Talk:Team:IvyTech-South_Bend/9_September_2010 9]
| [http://2010.igem.org/Talk:Team:IvyTech-South_Bend/10_September_2010 10]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/11_September_2010&action=edit 11]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/12_September_2010&action=edit 12]
|
[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/13_September_2010&action=edit 13]
| [http://2010.igem.org/Talk:Team:IvyTech-South_Bend/14_September_2010 14]
| [http://2010.igem.org/Talk:Team:IvyTech-South_Bend/15_September_2010 15]
| [http://2010.igem.org/Talk:Team:IvyTech-South_Bend/16_September_2010 16]
| [http://2010.igem.org/Talk:Team:IvyTech-South_Bend/17_September_2010 17]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/18_September_2010&action=edit 18]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/19_September_2010&action=edit 19]
|
[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/20_September_2010&action=edit 20]
| [http://2010.igem.org/Talk:Team:IvyTech-South_Bend/21_September_2010 21]
| [http://2010.igem.org/Talk:Team:IvyTech-South_Bend/22_September_2010 22]
| [http://2010.igem.org/Talk:Team:IvyTech-South_Bend/23_September_2010 23]
| [http://2010.igem.org/Talk:Team:IvyTech-South_Bend/24_September_2010 24]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/25_September_2010&action=edit 25]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/26_September_2010&action=edit 26]
|
[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/27_September_2010&action=edit 27]
| [http://2010.igem.org/Talk:Team:IvyTech-South_Bend/28_September_2010 28]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/29_September_2010&action=edit 29]
| [http://2010.igem.org/Talk:Team:IvyTech-South_Bend/30_September_2010 30]
|
|
October
|
M | T | W | T | F | S | S
|
|
|
|
| [http://2010.igem.org/Talk:Team:IvyTech-South_Bend/1_October_2010 1]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/2_October_2010&action=edit 2]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/3_October_2010&action=edit 3]
|
[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/4_October_2010&action=edit 4]
| [http://2010.igem.org/Talk:Team:IvyTech-South_Bend/5_October_2010 5]
| [http://2010.igem.org/Talk:Team:IvyTech-South_Bend/6_October_2010 6]
| [http://2010.igem.org/Talk:Team:IvyTech-South_Bend/7_October_2010 7]
| [http://2010.igem.org/Talk:Team:IvyTech-South_Bend/8_October_2010 8]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/9_October_2010&action=edit 9]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/10_October_2010&action=edit 10]
|
[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/11_October_2010&action=edit 11]
| [http://2010.igem.org/Talk:Team:IvyTech-South_Bend/12_October_2010 12]
| [http://2010.igem.org/Talk:Team:IvyTech-South_Bend/13_October_2010 13]
| [http://2010.igem.org/Talk:Team:IvyTech-South_Bend/14_October_2010 14]
| [http://2010.igem.org/Talk:Team:IvyTech-South_Bend/15_October_2010 15]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/16_October_2010&action=edit 16]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/17_October_2010&action=edit 17]
|
[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/18_October_2010&action=edit 18]
| [http://2010.igem.org/Talk:Team:IvyTech-South_Bend/19_October_2010 19]
| [http://2010.igem.org/Talk:Team:IvyTech-South_Bend/20_October_2010 20]
| [http://2010.igem.org/Talk:Team:IvyTech-South_Bend/21_October_2010 21]
| [http://2010.igem.org/Talk:Team:IvyTech-South_Bend/22_October_2010 22]
| [http://2010.igem.org/Talk:Team:IvyTech-South_Bend/23_October_2010 23]
| [http://2010.igem.org/Talk:Team:IvyTech-South_Bend/24_October_2010 24]
|
[http://2010.igem.org/Talk:Team:IvyTech-South_Bend/25_October_2010 25]
| [http://2010.igem.org/Talk:Team:IvyTech-South_Bend/26_October_2010 26]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/27_October_2010&action=edit 27]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/28_October_2010&action=edit 28]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/29_October_2010&action=edit 29]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/30_October_2010&action=edit 30]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/31_October_2010&action=edit 31]
|
|
November
|
M | T | W | T | F | S | S
|
[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/1_November_2010&action=edit 1]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/2_November_2010&action=edit 2]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/3_November_2010&action=edit 3]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/4_November_2010&action=edit 4]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/5_November_2010&action=edit 5]
| [http://2010.igem.org/Talk:Team:IvyTech-South_Bend/6_November_2010 6]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/7_November_2010&action=edit 7]
|
[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/8_November_2010&action=edit 8]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/9_November_2010&action=edit 9]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/10_November_2010&action=edit 10]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/11_November_2010&action=edit 11]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/12_November_2010&action=edit 12]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/13_November_2010&action=edit 13]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/14_November_2010&action=edit 14]
|
[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/15_November_2010&action=edit 15]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/16_November_2010&action=edit 16]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/17_November_2010&action=edit 17]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/18_November_2010&action=edit 18]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/19_November_2010&action=edit 19]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/20_November_2010&action=edit 20]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/21_November_2010&action=edit 21]
|
[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/22_November_2010&action=edit 22]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/23_November_2010&action=edit 23]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/24_November_2010&action=edit 24]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/25_November_2010&action=edit 25]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/26_November_2010&action=edit 26]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/27_November_2010&action=edit 27]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/28_November_2010&action=edit 28]
|
[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/29_November_2010&action=edit 29]
| [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/30_November_2010&action=edit 30]
|
|
IGEM Part #s and Uses
BBa_T9002 – Producer Controler Device–Plate2 Well 9A
BBa_I732017 – LacZ with our GFP 1—Plate 2 Well 3K
BBa_F2620– LuxR with terminator– Plate 2 Well 6E
BBa_I13522 –Tet Prop with GFP—Plate 2 Well 8A
BBa_T13521 Red Flouro tet RFP—Plate 2 Well 6O
BBa_pSB1C3—Plasmid—Plate 1 Well 3A
BBa_pSB1T3—Tetracycline resistant plasmid—Plate 1 Well 7A
BBa_pSB1AT3--high copy number plasmid carrying ampicillin and tetracycline resistance--Plate 1 Well 13A
6/28/10
Preparing SOB
6/30/10
Richard Lab:Electroporation of E. coli
7/28/10
Usage and extraction
8/24/10
Today we will be pouring new plates for streaking new transformed cells.
8/27/10
We will be running a gel to determine if our DNA sample was successfully electroplated.
8/27/10
Today we will be electroplating part KBBa_3131010
8/31/10
Results of transformation - the plates had growth but (no) distinct colony pattern.
8/31/10
After agar is dissolved, and allowed to cool, I will add Amp in different doses to specific plates. JH
9/1/10
Today the team will be making up the same plates as I made yesterday. Professor T. wants new Amp stock made to document how much is used into the stock.
9/2/10
Results from streaking –
9/2/10
Protocol For Making LB-Broth/Agar
9/3/10
Today I’m finishing making the LB/Agar from yesterday.
9/9/10
Due to conflict we will be changing from E Coli to yeast.
9/10/10
Lux Casette Right Promoter BBa_I1051
9/14/10
Today we found growth from our Electroporated E – Coli parts
9/15/10
Today we will be extracting DNA from out electroporated cells/T9002
B-galactosidase Assay
9/16/10
Today we will wash
9/17/10
Today we will be doing a DNA Extraction from/of part T9002 for electroporation this will determine if we have a stable host for our beast this will also Work!
9/21/10
Today we will electroporate Agrobacterium with part T9002 using protocol from pg 22 – 23
9/22/10
Today we have growth from electroporation done yesterday we are going to test by hitting with (AHL) the 20 mL grew not 5 mL sample
9/22/10
The only one that should glow should be (C)/(3) E – Coli w/transformed part in LB For Supernate of A.Tumafaciens
9/23/10
Today I have been setting up for the trial of our part T9002 inside agrobacteria to see if in the presence of AHL it floureces.
9/24/10
Today I’m testing absorbance of our trials @ 395nm
9/28/10
Today we will be picking 2 colonies from the Lacz, LuxR electroporated plates that were streaked on 9/22/10
9/30/10
LacZ without GFP also with RBS and terminator
10/1/10
electrophoresis
10/5/10
pTet GFP
10/7/10
Electroporating 2 parts with E-coli bacteria
10/8/10
LB-Lennox Broth & Electroporation
10/12/10
hold until Friday
10/15/10
Using openwetware.org protocol
B-galactosidase Assay
BBL Trypticase soy Broth and Agar
10/15/10
B-galactosidase Assay
10/19/10
Protocol-- Electroporation man for E-Coli
10/21/10
High Efficiency Electrotransformation of E.coli
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