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LB agar plates

Materials required

The materials mentioned below is used to make up 1 liter of the standard LB agar plates:

1. 10 g of tryptoptan
2. 5 g of yeast
3. 5 g of NaCl
4. 15 g of agar

The materials mentioned is used to make up 1 liter of LB starch agar plates:

1. 10 g of tryptoptan
2. 5 g of yeast
3. 5 g of NaCl
4. 15 g of agar
5. 10 g of starch

Protocol

• Dissolve the materials in 800 ml of distilled water. ( Note : The agar will not dissolve in distill water. )
• After most of the materials are dissolved, top up the finial volume to 1 liter using distilled water.
• Autoclave the mixture.
• After autoclaving, allow the mixture to cool down before adding the appropriate antibiotic.
• Pour appropriately 25 ml of the mixture into petri dishes.

Note: Aseptic technique must be employed after the autoclave part.

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