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Team:TU Delft/23 June 2010 content
From 2010.igem.org
Revision as of 08:55, 6 July 2010 by Ptmvanboheemen (Talk | contribs)
Lab Work
Ligations were performed using the digested BioBricks obtained in the early morning. We following ligation was performed:
| Componet | Sample |
| I10341 | 3 μL |
| J611XX | 4 μL |
| pSB3C5 | 1.5 μL |
| Ligation Buffer | 3 μL |
| T4 ligase | 1 μL |
| H20 | 15 μL |
We adhered to the 3:1 insert:plasmid ratio when determining the volumes of DNA added. The negative control for the ligation consisted of J61100 + pSB3C5. The mixtures were incubated overnight at 16 degrees.
