Team:Caltech/BBa K338003

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iGEM 2010

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<partinfo>BBa_K338003 short</partinfo>

This is half of a planned part which would contain all three PHA synthase genes required to produce [http://en.wikipedia.org/wiki/Polyhydroxybutyrate polyhydroxybutyrate] (PHB) in cells: phaA, phaB1, phaC1. This half contains only phaA: <partinfo>BBa_K156012</partinfo>. It was designed to be ligated upstream of part BBa_K338004.

Usage and Biology

Design

When ligated upstream of BBa_K338004, the completed construct was designed to express all three PHA synthase genes required to make PHB from soybean oil.

BioBrick Characterization

Effect of Heat Shock Temperature

Effect of Heat Shock Duration

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