Team:Heidelberg/Modeling/miBSdesigner

Revision as of 09:26, 26 October 2010 by ThomasU (Talk | contribs)

BS designer for miRNA

Construction of microRNA binding sites

Sequence name

microRNA Sequence ( 5' -> 3')

Spacer (inert sequence)

Perfect Binding Site
Imperfect Binding Site with 4 nt bulge (9-12)
Personalized Binding Site

Seed sequence (1-8)

Customized MISMATCH position

Supplementary region

Customized MATCH positions

Modify AU content

A in position -1
A in position 1
A in position 9

A in position 0
A in position 8
A in position 10

Sticky ends for integration into plasmid

Customized:
5'
3'

Name of primer 1

Sequence of primer 1

Name of primer 2

Sequence of primer 2