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Team:Chiba/Notebook 3
From 2010.igem.org
Lux inverter
<html><h3>2010-10-8</h3></html> transformation BBa_R0061 BBa_E0240 <br> <html><h3>2010-10-9</h3></html> Mini-prep BBa_R0061 BBa_E0240 digestion BBa_R0061 20 µL 10×Buffer 3 µL SpeI 1 µL PstI 1 µL dH<sub>2</sub>O5 µL -------------------- -------------------- BBa_E0240 20 µL 10×Buffer 3 µL XbaI 1 µL PstI 1 µL dH<sub>2</sub>O 5 µL -------------------- gel extraction <br> <html><h3>2010-10-11</h3></html> Ligation BBa_R0061 (70 ng) 1 µL BBa_E0240 (100 ng) 2 µL ATP (10 mM) 1 µL 10×Buffer 1 µL T4 DNA Ligase 1 µL dH<sub>2</sub>O 4 µL transformation <html><h3>2010-10-12</h3></html> Ligation BBa_R0061(100 ng) 1 µL BBa_E0240 (100 ng) 3 µL ATP (10 mM) 1 µL T4 DNA Ligase 1 µL dH<sub>2</sub>O 4 µL <html><h3>2010-10-13</h3></html>
