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Team:Brown/Project
From 2010.igem.org
Project 1 - Quad-state light-activation
Project Description
The aim of this project is to create a cell that can go through a sequence of four distinct states in response to a timed pattern of light-on, light-off.
This project makes use of parts from EPF Lusanne '09 (LOVTAP light activated promoter), PKU '07/'09 (bistable switch, AND gate), and Missouri Western State University '08 (Hybrid promoter). Our goal is to show that it is possible and easy to create entirely new cell logic systems from parts that already exist in the registry.
See the model for this system here:Team:Brown/Modeling
Progress
Project 2 - TAT-PTD
Project Description
We have created a recombinant protein which fuses a Tat-protein transduction domain (Tat-ptd) with a synthetic single chain variable fragment (scFv) domain. The goals of this project are: 1) be able to stably express the fusion protein in E. Coli; 2) create a protein that will retain conformation and function in cytosolic conditions; 3) the intrabody should be able to bind with high specificity to the desired target protein; 4) the intrabody can translocate across mammalian cell and tissue barriers; and 5) include a method for easy fluorescence reporting.
We also are in the process of creating a biobrick that will allow for easy fusion of the Tat-PTD to another biobricked proteins. We plan on characterizing this biobrick by combining it with transcription factors in the Registry and transiently acting on inducible/repressible reporter constructs.
