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Team:Alberta/Kit
From 2010.igem.org
Contents |
GENOMIKON
The Idea
GENOMIKON evolved from a team objective to bring synthetic biology to high school students. This proved to be a tall task as a high school environment has many limitations that don't apply to university research labs.
To work GENOMIKON must be:
- Require very little expensive equipment (autoclaves, centrifuges, -70C freezers)
- Fast
- Efficicent
- Easily shipped
- Inexpensive
The limitations imposed by the high school requirements forced our team to design a new standard for the assembly and production of DNA parts, BioBytes 2.0. The BioByte 2.0 standard solved most of the problems.
BioBytes 2.0 is:
- Fast-Parts can be added to a growing construct in 7 minutes.
- Efficient-It has been modelled to be 96% efficient. We have also used BioBytes 2.0 to create plasmids that have been transformed successfully into E. coli.
- Equipment-It does not require centrifugation or any other expensive equipment inherently.
The other limitations were solved by using innovative protocols and products:
- Equipment-Expensive micropipetters are replaced with precision droppers, which can accurately dispense small volumes of solutions.
- Easily Shipped-We will utilize lyophilized competent cells so that cold temperatures are not neccesary.
- Inexpensive-Based on our distribution analysis the kit will be inexpensive to produce and can be sold at costs that are lower than currently used high school biology labs.
