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Bacillus sphaericus Rehydration

Aims

We aimed to rehydrate and plate the natto strain (Bacillus subtilis ATCC 31578) bought from DSMZ, Germany. We used Penassay G- Thy Medium to rehydrate the cells. The cells are going to be used to test the strength of PGA glue.

Materials

• Pennassay G- Thy medium:

1. Pennassay broth- 17.5g
2. Glucose- 20g
3. Thymine- 0.05g
4. Distilled water- 1000ml

• Pennassay broth( per l of water):

1. peptone- 10g
2. Yeast extract- 1.5g
3. Sodium chloride- 3.5g
4. Glucose- 1.0g
5. Potassium phosphate dibasic- 3.68g
6. Potassium phospahte monobasic- 1.32g
7. Beef extract- 1.5g

ph7 store at 2-8 °C