Team:Edinburgh/Bacterial/Green light producer

• home
• team - illuminati
  • official
  • supervisors
  • advisors
  • students
  • environment
  • gallery
• genomic BRIDGEs
  • the protocol
  • submitted parts
  • results
  • future work
  • references
• bacterial BRIDGEs
  • the repressilator
  • red light
  • red sensor
  • blue light
  • blue sensor
  • green light
  • green sensor
  • submitted parts
  • results
  • future work
  • references
• modelling BRIDGEs
  • kappa
  • the genomic model
  • the bacterial model
  • the signalling model
  • tools
  • results
  • future work
  • references
• human BRIDGEs
  • human aspects
  • communication of science
  • terminology research
  • wiki
  • conversations
  • identity
  • collaboration
  • branding research
  • science fiction writing
  • future applications
  • self-reflection
  • the epic
  • results
  • future work
  • references
• lab notes   
  • collaboration
  • attribution
  • BRIDGE
  • red light
  • red sensor
  • blue light
  • blue sensor
  • green light
  • green sensor
  • modelling
  • safety
  • protocols
• acknowledgements

Overview: The green light producer

The green luciferase will require the combination of luxAB with the production of another protein: YFP from ''Vibrio fischeri''. This will shift the wavelength of the light towards the yellow spectrum, making it the correct colour to activate our green light sensor. Mexico UNAM-Genomics have had this DNA sequence synthesised and sending it to us for characterisation and combination with luxAB. We also expect Mexico to BioBrick it as part of their submission to the registry.

Strategy

***

Problems

You can't have problems with a piece of DNA you've never met.

BioBricks

Firefly luciferase was originally deposited in the Registry by Ljubljana 2007. We modified this slightly and re-submitted in pSB1C3, along with a codon-optimised mutant and a simple reporter system.

BBa_K322237: firefly luciferase from Photinus pyralis, modified BBa_I712019.

BBa_K322238: firefly luciferase from Photinus pyralis under lac promoter.

BBa_K322451: firefly luciferase from Photinus pyralis, codon optimised and mutated for brighter bioluminescence.

Characterisation

***

References

Fujii, H., Noda, K., Asami, Y., Kuroda, A., Sakata, M. & Tokida, A. (2007). Increase in bioluminescence intensity of firefly luciferase using genetic modification. Analytical Biochemistry 366, 131-136.