Team:Newcastle/Meetings/1 July 2010

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Contents 1 Roll call 2 Action points from last week 3 Research feedback - end of crack signalling, quorum sensing 4 Lab feedback

Roll call

Everyone here except Phil and Younas who are in the lab.

Action points from last week

• Wiki changes have been made.
• Letter to Andrew Young is not - someone needs to be in charge of this.
• Civil engineer funding is not done yet.
• Flights plan is to get there on Thursday about 4-5pm.

Research feedback - end of crack signalling, quorum sensing

Diagram on board. Oxygen limitation triggers subtilin production, Res D and E. Need subtilin immunity brick too spaIFEG. Subtilin receptor was done in 2008 by Newcastle spaRK. Want it to be triggered by both oxygen limitation and by subtilin. Oxygen to trigger the production at the end of the crack, subtilin to propagate the signal. Need to feedback subtilin to the production. Then it latches to production. Need to activate production in response to subtilin. Need the promotor at the end of the 2008 brick. Need a repressor to switch it off when oxygen is present. We need to get the strain with these genes. ATCC6633.

Lab feedback

Arginine and urease tests - Harsh and Alan. The experiment has been designed, waiting for the materials. In the lab at 4. Urea media, grown overnight, If the urea is used, there will be a colour change.

Arginine test. Bug in LB, supplement with arginine. If arginine is used, pH increase.

Phil and Y, transforming E. coli dh5alpha with the vector that we will be using for lacI. Plasmid extraction tomorrow.