Biobrick or
ligation name | Description | Creation date | Strain | Vector | Quality control | Length | Store |
|---|
|
<partinfo>BBa_E2050</partinfo>
|
mOrange
|
08/06/2010
|
E. coli DH5-alpha
|
<partinfo>pSB2K3</partinfo>
|
Seq ok(MIT); orange pellet when contained in <partinfo>pSB2K3</partinfo>
|
744 bp | BioBricks 2010 |
|
<partinfo>BBa_K165018</partinfo>
|
NLS-ADH1 terminator (Silver Standard 5' and 3')
|
08/06/2010
|
E. coli DH5-alpha
|
<partinfo>J63009</partinfo> (Amp)
|
Seq partially confirmed(MIT) because it lacks a T at the 3', but the ADH1 terminator (i.e. <partinfo>BBa_K165010</partinfo>) is ok. The correct ApE file is here.
|
253 bp | BioBricks 2010 |
|
<partinfo>BBa_K165037</partinfo>
|
tef2 promoter
|
08/06/2010
|
E. coli DH5-alpha
|
<partinfo>pSB1AK3</partinfo>
|
Seq ok(MIT)
|
403 bp | BioBricks 2010 |
|
<partinfo>BBa_P1004</partinfo>
|
Chloramphenicol resistence
cassette
|
08/06/2010
|
E. coli DH5-alpha
|
<partinfo>pSB1A1</partinfo>
|
Seq ok(MIT 2009); length ok
|
769 bp | BioBricks 2010 |
|
<partinfo>BBa_J61001</partinfo>
|
R6K origin
|
08/06/2010
|
E. coli DH5-alpha
|
<partinfo>pSB1A2</partinfo>
|
Seq ok(MIT)
|
406 bp | BioBricks 2010 |
|
<partinfo>BBa_K081008</partinfo>
|
RBS-luxI
|
08/06/2010
|
E. coli DH5-alpha
|
<partinfo>pSB1A2</partinfo>
|
Seq ok(MIT); length ok
|
664 bp | BioBricks 2010 |
|
<partinfo>BBa_K125500</partinfo>
|
GFP fusion brick (Silver Standard 5', Knight Standard 3')
|
08/06/2010
|
E. coli DH5-alpha
|
<partinfo>pSB1A2</partinfo>
|
Seq partially confirmed(MIT) for unknown reasons: the alignment is fully correct. The ApE file with the correct prefix and suffix is here. Length ok.
|
718 bp | BioBricks 2010 |
|
I0-1
|
<partinfo>BBa_K125500</partinfo> (E-S)
+
<partinfo>BBa_B0015</partinfo> (E-X)
|
11/06/2010
|
E. coli DH5-alpha
|
<partinfo>pSB1AK3</partinfo>
|
Wrong clone
|
| Ligations 2010 |
|
I0-2
|
<partinfo>BBa_K125500</partinfo> (E-S)
+
<partinfo>BBa_B0015</partinfo> (E-X)
|
11/06/2010
|
E. coli DH5-alpha
|
<partinfo>pSB1AK3</partinfo>
|
Seq ok(BMR); length ok
|
4036 bp | Ligations 2010 |
|
I1-1
(not used)
|
<partinfo>BBa_E2050</partinfo> (E-S)
+
<partinfo>BBa_K165018</partinfo> (E-X)
|
11/06/2010
|
E. coli DH5-alpha
|
<partinfo>J63009</partinfo> (Amp)
|
not sequenced, length ok
|
| Ligations 2010 |
|
I1-2
|
<partinfo>BBa_E2050</partinfo> (E-S)
+
<partinfo>BBa_K165018</partinfo> (E-X) |
11/06/2010
|
E. coli DH5-alpha
|
<partinfo>J63009</partinfo> (Amp)
|
Seq ok(BMR), with an additional barcode (25bp) after the stop codon of mOrange; length ok
|
| Ligations 2010 |
|
I2-1
|
<partinfo>BBa_P1004</partinfo> (E-S)
+
<partinfo>BBa_B0015</partinfo> (E-X)
|
11/06/2010
|
E. coli DH5-alpha
|
<partinfo>pSB1AK3</partinfo>
|
Seq ok(BMR); Cm resistance ok; length ok.
|
| Ligations 2010 |
|
I2-2
(not used)
|
<partinfo>BBa_P1004</partinfo> (E-S)
+
<partinfo>BBa_B0015</partinfo> (E-X) |
11/06/2010
|
E. coli DH5-alpha
|
<partinfo>pSB1AK3</partinfo>
|
not sequenced; Cm resistance ok
|
| Ligations 2010 |
|
I3-1
|
<partinfo>BBa_K081008</partinfo> (E-S)
+
<partinfo>BBa_B0015</partinfo> (E-X) |
11/06/2010
|
E. coli DH5-alpha
|
<partinfo>pSB1AK3</partinfo>
|
Seq TWO PLASMIDS IN THIS CLONE!(BMR), two overlapped traces; length ok
|
| Ligations 2010 |
|
I3-2
|
<partinfo>BBa_K081008</partinfo> (E-S)
+
<partinfo>BBa_B0015</partinfo> (E-X) |
11/06/2010
|
E. coli DH5-alpha
|
<partinfo>pSB1AK3</partinfo>
|
Wrong clone
|
| Ligations 2010 |
|
I4-1
(not used)
|
<partinfo>BBa_K165037</partinfo> (S-P)
+
I1 (X-P) |
18/06/2010
|
E. coli DH5-alpha
|
<partinfo>pSB1AK3</partinfo>
|
not sequenced; length ok
|
| Ligations 2010 |
|
I4-2
|
<partinfo>BBa_K165037</partinfo> (S-P)
+
I1 (X-P) |
18/06/2010
|
E. coli DH5-alpha
|
<partinfo>pSB1AK3</partinfo>
|
Seq ok(BMR); length ok
|
| Ligations 2010 |
|
I5
|
I2 (E-S)
+
<partinfo>BBa_J61001</partinfo> (E-X) |
18/06/2010
|
E. coli DH5-alpha
|
<partinfo>pSB1A2</partinfo>
|
Seq ok(BMR); Cm resistance ok; length ok
|
| Ligations 2010 |
|
I6-2
|
I3 (E-S)
+
<partinfo>BBa_T9002</partinfo> (E-X) |
18/06/2010
|
E. coli DH5-alpha
|
<partinfo>pSB1A3</partinfo>
|
Seq LONG PART(BMR), but the ligation worked and the sequenced portions are ok; length ok
|
| Ligations 2010 |
|
I6-3
(not used)
|
I3 (E-S)
+
<partinfo>BBa_T9002</partinfo> (E-X) |
18/06/2010
|
E. coli DH5-alpha
|
<partinfo>pSB1A3</partinfo>
|
not sequenced
|
| Ligations 2010 |
|
<partinfo>BBa_K208001</partinfo> (stab-1)
|
phasin phaP |
24/06/2010
|
E. coli ???
|
<partinfo>pSB3K3</partinfo>
|
length ok
|
579 bp | BioBricks 2010 |
|
<partinfo>BBa_K208001</partinfo> (stab-2)
|
phasin phaP |
24/06/2010
|
E. coli ???
|
<partinfo>pSB3K3</partinfo>
|
length ok
|
579 bp | BioBricks 2010 |
|
I7-1
|
<partinfo>BBa_J23118</partinfo> (E-S)
+
I6-2 (X-P) |
25/06/2010
|
E. coli DH5-alpha
|
<partinfo>BBa_J61002</partinfo>
|
none
|
| Ligations 2010 |
|
I7-2
|
<partinfo>BBa_J23118</partinfo> (E-S)
+
I6-2 (X-P) |
25/06/2010
|
E. coli DH5-alpha
|
<partinfo>BBa_J61002</partinfo>
|
none
|
| Ligations 2010 |
|
I8-1
|
<partinfo>BBa_J23110</partinfo> (E-S)
+
I6-2 (X-P) |
25/06/2010
|
E. coli DH5-alpha
|
<partinfo>BBa_J61002</partinfo>
|
none
|
| Ligations 2010 |
|
I8-2
|
<partinfo>BBa_J23110</partinfo> (E-S)
+
I6-2 (X-P) |
25/06/2010
|
E. coli DH5-alpha
|
<partinfo>BBa_J61002</partinfo>
|
none
|
| Ligations 2010 |
|
I9-1
|
<partinfo>BBa_J23111</partinfo> (E-S)
+
I6-2 (X-P) |
25/06/2010
|
E. coli DH5-alpha
|
<partinfo>BBa_J61002</partinfo>
|
none
|
| Ligations 2010 |
|
I9-2
|
<partinfo>BBa_J23111</partinfo> (E-S)
+
I6-2 (X-P) |
25/06/2010
|
E. coli DH5-alpha
|
<partinfo>BBa_J61002</partinfo>
|
none
|
| Ligations 2010 |
|
I10-1
|
<partinfo>BBa_J23116</partinfo> (E-S)
+
I6-2 (X-P) |
25/06/2010
|
E. coli DH5-alpha
|
<partinfo>BBa_J61002</partinfo>
|
none
|
| Ligations 2010 |
|
I10-2
|
<partinfo>BBa_J23116</partinfo> (E-S)
+
I6-2 (X-P) |
25/06/2010
|
E. coli DH5-alpha
|
<partinfo>BBa_J61002</partinfo>
|
none
|
| Ligations 2010 |
"
