Team:UNIPV-Pavia/Freezer

From 2010.igem.org

FREEZER MANAGEMENT



For a detailed correspondence between our BioBrick "wiki names" and submitted BioBrick codes take a look here.

Biobrick name Description Creation date Strain Vector Quality control Length Store

BBa_E2050

mOrange

08/06/2010

E. coli DH5-alpha

pSB2K3

Seq ok (MIT); orange pellet when contained in pSB2K3

744 bpBioBricks 2010

BBa_K165018

NLS-ADH1 terminator (Silver Standard 5' and 3')

08/06/2010

E. coli DH5-alpha

BBa_J63009 (Amp)

Seq partially confirmed (MIT) because it lacks a T at the 3', but the ADH1 terminator (i.e. BBa_K165010) is ok. The correct ApE file is here.

253 bpBioBricks 2010

BBa_K165037

tef2 promoter

08/06/2010

E. coli DH5-alpha

pSB1AK3

Seq ok (MIT).

403 bpBioBricks 2010

BBa_P1004

Chloramphenicol resistence
cassette

08/06/2010

E. coli DH5-alpha

pSB1A1

Seq ok (MIT 2009); length ok.

769 bpBioBricks 2010

BBa_J61001

R6K origin

08/06/2010

E. coli DH5-alpha

pSB1A2

Seq ok (MIT).

406 bpBioBricks 2010

BBa_K081008

RBS-luxI

08/06/2010

E. coli DH5-alpha

pSB1A2

Seq ok (MIT); length ok.

664 bpBioBricks 2010

BBa_K125500

GFP fusion brick (Silver Standard 5', Knight Standard 3')

08/06/2010

E. coli DH5-alpha

pSB1A2

Seq partially confirmed (MIT) for unknown reasons: the alignment is fully correct. The ApE file with the correct prefix and suffix is here. Length ok.

718 bpBioBricks 2010

BBa_K208001
(stab-1)

phasin phaP
(Silver Standard 5' and 3')

24/06/2010

E. coli ???

pSB3K3

Seq ok (BMR); length ok.

579 bpBioBricks 2010

BBa_K208001
(stab-2)

phasin phaP
(Silver Standard 5' and 3')

24/06/2010

E. coli ???

pSB3K3

Seq ok (BMR); length ok.

579 bpBioBricks 2010

BBa_J72007

BamHI methyltransferase encoding CRIM plasmid

01/07/2010

E. coli ???

HC Cm resistance

No plasmid in the clone.
For more informations click here.

BioBricks 2010

BBa_J72008

phi80 integration helper plasmid pInt80-649

01/07/2010

E. coli ???

Plasmid (Amp 50 ug/ml resistance)

Length ok.

6335 bpBioBricks 2010

BBa_J72013

BglII methyltransferase encoding CRIM plasmid

01/07/2010

E. coli ???

HC Cm resistance

No plasmid in the clone.
For more informations click here.

BioBricks 2010

BBa_K105012

10 aa flexible protein domain linker

06/08/2010

E. coli DH5-alpha

pSB1A2

-

30 bp BioBricks 2010

BBa_J13002

p(tetR)+RBS34

16/08/2010

E. coli TOP10

pSB1A2

-

74 bp BioBricks 2010

BBa_I13522

Untagged GFP behind a constitutive promoter

16/08/2010

E. coli TOP10

pSB1A2

-

937 bp BioBricks 2010
pSB1C3_RFP

BBa_J04450
in pSB1C3.

??

E. coli DH5alpha

pSB1C3

1069 bp

2009 Distribution
pSB1C3_ccdB

BBa_P1010
in pSB1C3.

??

E. coli DH5alpha

pSB1C3

675 bp

2009 Distribution
Name Ligation Creation date Strain Vector Quality control Length Description

I0
(it was I0-2)
BBa_K300005

BBa_K125500 (E-S)
+
BBa_B0015 (E-X)

11/06/2010

E. coli DH5-alpha

pSB1AK3

Seq ok (BMR); length ok.

854 bp GFPs + TT

I1
(it was I1-2)
BBa_K300006

BBa_E2050 (E-S)
+
BBa_K165018 (E-X)

11/06/2010

E. coli DH5-alpha

BBa_J63009 (Amp)

Seq ok (BMR), with an additional barcode (25bp) after the stop codon of mOrange; length ok.

1029 bp mOrange + NSL-ADH1 terminator

I2
(it was I2-1)

BBa_P1004 (E-S)
+
BBa_B0015 (E-X)

11/06/2010

E. coli DH5-alpha

pSB1AK3

Seq ok (BMR); Cm resistance ok; length ok.

906 bp Cm resistance + TT

I3
(it was I3-1)
BBa_K300009

BBa_K081008 (E-S)
+
BBa_B0015 (E-X)

11/06/2010

E. coli DH5-alpha

pSB1AK3

Seq TWO PLASMIDS IN THIS CLONE!(BMR), two overlapped traces; length ok.

801 bp RBS + luxI + TT

I4
(it was I4-2)

BBa_K165037 (S-P)
+
I1 (X-P)

18/06/2010

E. coli DH5-alpha

pSB1AK3

Seq ok (BMR); length ok.

1439 bp pTEF + mOrange + NLS + terminator

I5
(it was I5-1)
BBa_K300008

I2 (E-S)
+
BBa_J61001 (E-X)

18/06/2010

E. coli TOP10

pSB1A2

Seq ok (BMR); Cm resistance ok; length ok.

1320 bp Cm resistance + TT + R6K ori

I6
(it was I6-2)

I3 (E-S)
+
BBa_T9002 (E-X)

18/06/2010

E. coli DH5-alpha

pSB1A3

Seq LONG PART(BMR), but the ligation worked and the sequenced portions are ok; length ok.

2754 bp RBS + luxI + TT + HSL sensor

I7
(it was I7-3)
BBa_K300024

BBa_J23118 (S-P)
+
I6 (X-P)

25/06/2010

E. coli TOP10

BBa_J61002
(Amp)

Length ok.

2795 bp constitutive promoter + RBS + luxI + TT + HSL sensor

I8
(it was I8-5)
BBa_K300021

BBa_J23110 (S-P)
+
I6 (X-P)

25/06/2010

E. coli TOP10

BBa_J61002
(Amp)

Length ok.

2795 bp constitutive promoter + RBS + luxI + TT + HSL sensor

I9
(it was I9-1)
BBa_K300022

BBa_J23114 (S-P)
+
I6 (X-P)

25/06/2010

E. coli TOP10

BBa_J61002
(Amp)

Length ok.

2795 bp constitutive promoter + RBS + luxI + TT + HSL sensor

I10
(it was I10-1)
BBa_K300023

BBa_J23116 (S-P)
+
I6 (X-P)

25/06/2010

E. coli TOP10

BBa_J61002
(Amp)

Length ok.

2795 bp constitutive promoter + RBS + luxI + TT + HSL sensor

I12
(it was I12-2)
BBa_K300019

BBa_J23105 (S-P)
+
I6 (X-P)

16/07/2010

E. coli TOP10

BBa_J61002
(Amp)

Seq ok (BMR), length ok.

2795 bp constitutive promoter + RBS + luxI + TT + HSL sensor

I14
(it was I14-1)
BBa_K300030

BBa_J23118 (S-P)
+
I3 (X-P)

16/07/2010

E. coli DH5-alpha

BBa_J61002
(Amp)

Seq ok (BMR), length ok.

842 bp constitutive promoter + RBS + luxI + TT

RING

I5 (X-S) on itself:
Cm resistance (BBa_P1004) + TT (BBa_B0015) + R6K ori (BBa_J61001)

16/07/2010

E. coli DH5-alpha

Plasmid (Cm resistance, conditional thermosensitive ORI; works in PIR strains)

Tested here.

1328 bp

Cm resistance + TT + R6K ori

I15
(it was I15-1)
BBa_K300028

BBa_J23110 (S-P)
+
I3 (X-P)

23/07/2010

E. coli DH5-alpha

BBa_J61002
(Amp)

Length ok.

842 bp constitutive promoter + RBS + luxI + TT

I16
(it was I16-1)
BBa_K300029

BBa_J23116 (S-P)
+
I3 (X-P)

16/07/2010

E. coli DH5-alpha

BBa_J61002
(Amp)

Seq ok (BMR), length ok.

842 bp constitutive promoter + RBS + luxI + TT

I17
(it was I17-1)
BBa_K300025

BBa_J23101 (S-P)
+
I3 (X-P)

16/07/2010

E. coli DH5-alpha

BBa_J61002
(Amp)

Seq ok (BMR), length ok.

842 bp constitutive promoter + RBS + luxI + TT

I18
(it was I18-1)
BBa_K300026

BBa_J23105 (S-P)
+
I3 (X-P)

16/07/2010

E. coli DH5-alpha

BBa_J61002
(Amp)

Seq ok (BMR), length ok.

842 bp constitutive promoter + RBS + luxI + TT

I19
(it was I19-1)
BBa_K300027

BBa_J23106 (S-P)
+
I3 (X-P)

16/07/2010

E. coli DH5-alpha

BBa_J61002
(Amp)

Seq ok (BMR), length ok.

842 bp constitutive promoter + RBS + luxI + TT

I20
(it was I20M-1)
BBa_K300002

Pha10S-1 (X-S) (infos here)
+
pSB1AK3 (X-S)

13/08/2010

E. coli DH5-alpha

pSB1AK3

Seq ok (BMR), length ok.

576 bp

Pha10S

I21
(it was I21-4)
BBa_K300003

PhaSS-1 (X-S) (infos here)
+
pSB1A3 (X-S)

10/08/2010

E. coli DH5-alpha

pSB1A3

Seq ok (BMR), length ok.

576 bp

PhaSS

I22
(it was I22-1)
BBa_K300031

BBa_K081009 (E-S)
+
BBa_B0015 (E-X)

10/08/2010

E. coli DH5-alpha

pSB1AK3

Length ok.

825 bp

RBS + lasI + terminator

I23
(it was I23-1)

BBa_R0062 (S-P)
+
I3 (X-P)

10/08/2010

E. coli DH5-alpha

pSB1A2

Length ok.

725 bp

plux + RBS + luxI + TT

I24
(it was I24-1)

BBa_R0062 (S-P)
+
I22 (X-P)

13/08/2010

E. coli DH5-alpha

pSB1A2

Length ok.

886 bp

plux + RBS + lasI + terminator

I26
(it was I26-2)
BBa_K300081

BBa_K105012 (S-P)
+
I21 (X-P)

13/08/2010

E. coli DH5-alpha

pSB1A2

Seq ok (BMR), length ok.

612 bp

linker + PhaSS

I29
(it was I29-1)

BBa_T9002 (S-P)
+
I23 (X-P)

31/08/2010

E. coli TOP10

pSB1A3

Length ok.

2678 bp

HLS sensor + plux + RBS + luxI + TT

I30

BBa_T9002 (S-P)
+
I24 (X-P)

E. coli TOP10

pSB1A3

2839 bp

HSL sensor + plux + RBS + lasI + terminator

I31
(it was I31-1)
BBa_K300079

I20 (E-S)
+
BBa_K105012 (E-X)

23/08/2010

E. coli DH5-alpha

pSB1A2

Seq ok (BMR), length ok.

612 bp

Pha10S + linker

I32
(it was I32-2)

I20 (E-S)
+
I21 (E-X)

23/08/2010

E. coli DH5-alpha

pSB1A3

Seq ok (BMR), length ok.

1158 bp

Pha10S + PhaSS

I33
(it was I33-1)

I20 (E-S)
+
I0 (E-X)

23/08/2010

E. coli DH5-alpha

pSB1AK3

Seq ok (BMR), length ok.

1436 bp

Pha10S + GFPs + TT

I34
(it was I34-1)

I21 (E-S)
+
I0 (E-X)

23/08/2010

E. coli DH5-alpha

pSB1AK3

Seq ok (BMR), length ok.

1436 bp

PhaSS + GFPs + TT

I35
(it was I35-1)
BBa_K300080

I21 (E-S)
+
BBa_K105012 (E-X)

23/08/2010

E. coli DH5-alpha

pSB1A2

Seq ok (BMR), length ok.

612 bp

PhaSS + linker

I36
(it was I36-7)

I21 (E-S)
+
I21 (E-X)

24/08/2010

E. coli DH5-alpha

pSB1A3

Length ok.

1158 bp

PhaSS + PhaSS

I37
(it was I37-1)

INTEIN (E-S)
+
I0 (E-X)

23/08/2010

E. coli DH5-alpha

pSB1AK3

Seq ok (BMR), length ok.

1367 bp

INTEIN + GFPs + TT

I38
(it was I38-3)

BBa_F2620 (E-S)
+
BBa_B0034 (E-X)

23/08/2010

E. coli DH5-alpha

pSB1A2

Seq ok (BMR), length ok.

1081 bp

HSL inducible system

I39
(it was I39-5)

BBa_J13002 (S-P)
+
I20 (X-P)

24/08/2010

E. coli DH5-alpha

pSB1A2

Seq ok (BMR), length ok.

656 bp

pTet + Pha10S

I40
(it was I40-1)

I26 (E-S)
+
INTEIN (E-X)

25/08/2010

E. coli DH5-alpha

pMA (HC Amp resistance)

Length ok.

1125 bp

linker + PhaSS + INTEIN

I41
(it was I41-1)

I26 (E-S)
+
I0 (E-X)

23/08/2010

E. coli DH5-alpha

pSB1AK3

Seq ok (BMR), length ok.

1472 bp

linker + PhaSS + GFPs + TT

I42
(it was I42-3)

CREAM (E-P)
+
BBa_K173001 (E-P)

23/08/2010

E. coli BW23474

CREAM

Length ok.

3068 bp

CREAM fragment + Cm resistance + TT + R6K ori + constitutive GFP

I43

(it was I43-2)

CREAM (E-P)
+
BBa_J23101 (E-P)

23/08/2010

E. coli BW23474

CREAM

Length ok.

3026 bp

CREAM fragment + Cm resistance + TT + R6K ori + constitutive promoter

I44
(it was I44-2)

CREAM (E-P)
+
BBa_I52002 (E-P)

15/09/2010

E. coli DB3.1

CREAM

Seq ok (BMR).

3239 bp

CREAM fragment + Cm resistance + TT + R6K ori + marker ccdB

I45
FAILED

CREAM (HindIII) on itself

23/08/2010

E. coli BW23474

CREAM

2149 bp

CREAM fragment + Cm resistance + TT + R6K ori

I47
(it was I47-13)
BBa_K300086

BBa_J13002 (S-P) + I33 (X-P)

02/09/2010

E. coli TOP10

pSB1A2

Seq ok (BMR), length ok.

1516 bp

pTet + Pha10S + GFPs + TT

I48
(it was I48-1)
BBa_K300088

I39 (S-P) + I34 (X-P)

31/08/2010

E. coli TOP10

pSB1A2

Seq ok (BMR), length ok.

2098 bp

pTet + Pha10S + PhaSS + GFPs + TT

I49
(it was I49-1)
BBa_K300090

I39 (S-P) + I41 (X-P)

31/08/2010

E. coli TOP10

pSB1A2

Seq ok (BMR), length ok.

2134 bp

pTet + Pha10S + linker + PhaSS + GFPs + TT

I50
FAILED (no trasformants on plate)

I44 (E-H) on itself

28/08/2010

E. coli DB3.1

CREAM

3239 bp

CREAM fragment + Cm resistance + TT + R6K ori + marker ccdB

I51
(it was I51-3)
BBa_I52002

CREAM (E-P)
+
BBa_I52002 (E-P)

03/09/2010

E. coli DB3.1

CREAM

3239 bp

CREAM fragment + Cm resistance + TT + R6K ori + marker ccdB

I52
(it was I52-c)

I35 (S-P)
+
I37 (X-P)

17/09/2010

E. coli DH5-alpha

pSB1A2

Length ok.

1985 bp

PhaSS + linker + INTEIN + GFPs + TT

I53
(it was I53-c)

I32 (E-S)
+
I37 (E-X)

17/09/2010

E. coli DH5-alpha

pSB1AK3

Length ok.

2531 bp

Pha10S + PhaSS + INTEIN + GFPs + TT

I54
(it was I54-a)

I36 (E-S)
+
I37 (E-X)

17/09/2010

E. coli DH5-alpha

pSB1AK3

Length ok.

2531 bp

PhaSS + PhaSS + INTEIN + GFPs + TT

I55
(it was I55-1)
BBa_K300082

I38 (E-S)
+
I20 (E-X)

02/10/2010

E. coli DH5-alpha

pSB1AK3

Seq ok (BMR), length ok.

1663 bp

HSL inducible system + Pha10S

I56
(it was I56-a)

I26 (E-S)
+
I41 (E-X)

17/09/2010

E. coli DH5-alpha

pSB1AK3

Length ok.

2090 bp

linker + PhaSS + linker + PhaSS + GFPs + TT

I57
(it was I57-a)

I40 (E-S)
+
I0 (E-X)

17/09/2010

E. coli DH5-alpha

pSB1AK3

Length ok.

1985 bp

linker + PhaSS + INTEIN + GFPs + TT

I58
(it was I58-c)

I38 (E-S)
+
I31 (E-X)

17/09/2010

E. coli DH5-alpha

pSB1A2

Length ok.

1699 bp

HSL inducible system + Pha10S + linker

I59
(it was I59-b)

I21 (E-S)
+
I34 (E-X)

17/09/2010

E. coli DH5-alpha

pSB1AK3

Length ok.

2018 bp

PhaSS + PhaSS + GFPs + TT

I60
(it was I60-a)

I20 (S-P)
+
I26 (X-P)

17/09/2010

E. coli DH5-alpha

pSB1AK3

Seq ok (BMR), length ok.

1194 bp

Pha10S + linker + PhaSS

I61
(it was I61-a)

I31 (E-S)
+
I37 (E-X)

17/09/2010

E. coli DH5-alpha

pSB1AK3

Length ok.

1985 bp

Pha10S + linker + INTEIN + GFPs + TT

I62
(it was I62-E)

I39 (S-P)
+
I59 (X-P)

27/09/2010

E. coli TOP10

pSB1A2

Length ok.

2678 bp

pTet + Pha10S + PhaSS + PhaSS + GFPs + TT

I63
(it was I63-1)
BBa_K300091

I38 (E-S)
+
I61 (E-X)

24/09/2010

E. coli TOP10

pSB1AK3

Length ok.

3072 bp

HSL inducible system + Pha10S + linker + INTEIN + GFPs + TT

I64
(it was I64-2)
BBa_K300092

I58 (E-S)
+
I52 (E-X)

24/09/2010

E. coli TOP10

pSB1A2

Length ok.

3690 bp

HSL inducible system + Pha10S + linker + PhaSS + linker + INTEIN + GFPs + TT

I65
(it was I65-3)
BBa_K300099

I39 (S-P)
+
I56 (X-P)

24/09/2010

E. coli TOP10

pSB1A2

Length ok.

2752 bp

pTet + Pha10S + linker + PhaSS + linker + PhaSS + GFPs + TT

I66
(it was I66-1)

I60 (E-S)
+
I57 (E-X)

24/09/2010

E. coli TOP10

pSB1AK3

Length ok.

3185 bp

Pha10S + linker + PhaSS + linker + PhaSS + INTEIN + GFPs + TT

I70
(it was I70-1)
BBa_K300007

MyYeast3 (E-S)
+
I4 (E-S)

01/10/10

E. coli TOP10

MyYeast (Amp100 in coli)

5187bp

MyYeast vector + I4 in cloning site (Ptef1 + mOrange + Tadh1)

I71
(it was I71-1)

MyYeast3 (E-S)
+
I1 (E-S)

01/10/10

E. coli TOP10

MyYeast (Amp100 in coli)

4778bp

MyYeast vector + I1 in cloning site (mOrange + Tadh1, NO promoter)

I72
(it was I70-x)

BBa_J04450(E-P)+pSB4C5(E-P)

E. coli TOP10

pSB4C5

4290bp

mRFP under pLac promoter in LC plasmid pSB4C5

I73
(it was I73-1)

I38 (E-S)
+
I53 (E-X)

02/10/2010

E. coli TOP10

pSB1AK3

Length ok.

3618 bp

HSL inducible system + Pha10S + PhaSS + INTEIN + GFPs + TT

I74
(it was I74-8)

I31 (E-S)
+
I35 (E-X)

04/10/2010

E. coli TOP10

pSB1A2

Length ok.

1230 bp

Pha10S + linker + PhaSS + linker

I75
(it was I75-x)

I55 (E-S)
+
I37 (E-X)

Not screened yet.

E. coli TOP10

pSB1AK3

Not screened yet.

3036 bp

HSL inducible system + Pha10S + INTEIN + GFPs + TT

I76
(it was I76-x)

I55 (E-S)
+
I54 (E-X)

Not screened yet.

E. coli DH5-alpha

pSB1AK3

Not screened yet.

4191 bp

HSL inducible system + Pha10S + PhaSS + PhaSS + INTEIN + GFPs + TT

I77
(it was I77-x)

I55 (E-S)
+
I57 (E-X)

Not screened yet.

E. coli TOP10

pSB1AK3

Not screened yet.

3654 bp

HSL inducible system + Pha10S + linker + PhaSS + INTEIN + GFPs + TT

I78
(it was I78-3)
BBa_K300084

I74 (E-S)
+
INTEIN_C3 (E-X)

07/10/10

E. coli TOP10

pSB1C3

Seq ok (BMR), length ok.

1743 bp

Pha10S + linker + PhaSS + linker + INTEIN

I79
(it was I79-2)
BBa_K300083

I60 (E-S)
+
INTEIN_C3 (E-X)

07/10/10

E. coli STBL3

pSB1C3

Seq ok (BMR), length ok.

1707 bp

Pha10S + linker + PhaSS + INTEIN

I80
(it was I80-4)
BBa_K300010

I3_1C3 (S-P)
+
BBa_F2620 (X-P)

11/10/10

E. coli TOP10

pSB1C3

Seq ok (BMR), length ok.

1707 bp

RBS + luxI + TT + BBa_F2620

INTEIN
BBa_K300004

Intein

13/08/2010

E. coli TOP10

pMA
(HC Amp resistance)

Mr.Gene DNA synthesis

507 bp

INTEIN

INTEIN_C3

Intein

02/10/2010

E. coli TOP10

pSB1C3

Mr.Gene DNA synthesis

507 bp

INTEIN in pSB1C3

YEAST
MR.G

BBa_K300980

YEAST fragment

15/09/2010

E. coli DH5-alpha

pMA
(HC Amp resistance)

Mr.Gene DNA synthesis

1695 bp

YEAST fragment

CRIM
MR.G

BBa_K300983

CREAM fragment

13/08/2010

E. coli TOP10

pMK-RQ
(HC Kan resistance)

Mr.Gene DNA synthesis

829 bp

CREAM fragment

CREAM
(it was MyCRIM)
BBa_K300982

CRIM
MR.G (A-A)
+
I5 (S-X)

21/08/2010

E. coli BW23474

CREAM

Length ok.

2149 bp

CREAM fragment + Cm resistance + TT + R6K ori

I7-4C5
(it was I7-4C5-2)

I7 (E-P)
+
pSB4C5(E-P)

16/07/2010

E. coli TOP10

pSB4C5

Seq ok (BMR), length ok.

2795 bp constitutive promoter + RBS + luxI + TT + HSL sensor in pSB4C5

I8-4C5
(it was I8-4C5-2)

I8 (E-P)
+
pSB4C5 (E-P)

16/07/2010

E. coli TOP10

pSB4C5

Seq ok (BMR), length ok.

2795 bp constitutive promoter + RBS + luxI + TT + HSL sensor in pSB4C5

I10-4C5
(it was I10-4C5-1)

I10 (E-P)
+
pSB4C5 (E-P)

16/07/2010

E. coli TOP10

pSB4C5

Length ok.

2795 bp

constitutive promoter + RBS + luxI + TT + HSL sensor in pSB4C5

I12-4C5
(it was I12-4C5-1)

I12 (E-P)
+
pSB4C5 (E-P)

25/07/2010

E. coli TOP10

pSB4C5

Length ok.

2795 bp

constitutive promoter + RBS + luxI + TT + HSL sensor in pSB4C5

I14-4C5
(it was I14-4C5-2)

I14 (E-P)
+
pSB4C5 (E-P)

23/07/2010

E. coli DH5-alpha

pSB4C5

Length ok.

842 bp

constitutive promoter + RBS + luxI + TT + HSL sensor in pSB4C5

I15-4C5
(it was I15-4C5-2)

I15 (E-P)
+
pSB4C5 (E-P)

13/08/2010

E. coli TOP10

pSB4C5

Length ok.

842 bp

constitutive promoter + RBS + luxI + TT in pSB4C5

I16-4C5
(it was I16-4C5-1)

I16 (E-P)
+
pSB4C5 (E-P)

23/07/2010

E. coli DH5-alpha

pSB4C5

Length ok.

842 bp

constitutive promoter + RBS + luxI + TT in pSB4C5

I17-4C5
(it was I17-4C5-1)

I17 (E-P)
+
pSB4C5 (E-P)

25/07/2010

E. coli DH5-alpha

pSB4C5

Length ok.

842 bp

constitutive promoter + RBS + luxI + TT in pSB4C5

I18-4C5
(it was I18-4C5-1)

I18 (E-P)
+
pSB4C5 (E-P)

25/07/2010

E. coli DH5-alpha

pSB4C5

Length ok.

842 bp

constitutive promoter + RBS + luxI + TT in pSB4C5

I19-4C5
(it was I19-4C5-1)

I19 (E-P)
+
pSB4C5 (E-P)

25/07/2010

E. coli DH5-alpha

pSB4C5

Length ok.

842 bp

constitutive promoter + RBS + luxI + TT in pSB4C5

I27

I23 (E-P)
+
pSB4C5 (E-P)

17/08/2010

E. coli TOP10

pSB4C5

725 bp

plux + RBS + luxI + TT in pSB4C5

I28

I24 (E-P)
+
pSB4C5 (E-P)

17/08/2010

E. coli TOP10

pSB4C5

886 bp

plux + RBS + lasI + terminator in pSB4C5

BBa_J23100
_4C5

(it was BBa_J23100
_4C5-1)

BBa_J23100
+
BBa_B0034
+
BBa_E1010
+
BBa_B0015 in pSB4C5.

27/08/2010

E. coli TOP10

pSB4C5

Seq ok (BMR), length ok.

904 bp

constitutive promoter + RBS + mRFP1 + TT in pSB4C5

BBa_J23101
_4C5

(it was BBa_J23101
_4C5-1)

BBa_J23101
+
BBa_B0034
+
BBa_E1010
+
BBa_B0015 in pSB4C5.

27/08/2010

E. coli TOP10

pSB4C5

Seq ok (BMR), length ok.

904 bp

constitutive promoter + RBS + mRFP1 + TT in pSB4C5

BBa_J23105
_4C5

(it was BBa_J23105
_4C5-1)

BBa_J23105
+
BBa_B0034
+
BBa_E1010
+
BBa_B0015 in pSB4C5.

27/08/2010

E. coli TOP10

pSB4C5

Length ok.

904 bp

constitutive promoter + RBS + mRFP1 + TT in pSB4C5

BBa_J23106
_4C5

(it was BBa_J23106
_4C5-1)

BBa_J23106
+
BBa_B0034
+
BBa_E1010
+
BBa_B0015 in pSB4C5.

27/08/2010

E. coli TOP10

pSB4C5

Length ok.

904 bp

constitutive promoter + RBS + mRFP1 + TT in pSB4C5

BBa_J23110
_4C5

(it was BBa_J23110
_4C5-1)

BBa_J23110
+
BBa_B0034
+
BBa_E1010
+
BBa_B0015 in pSB4C5.

27/08/2010

E. coli TOP10

pSB4C5

Length ok.

904 bp

constitutive promoter + RBS + mRFP1 + TT in pSB4C5

BBa_J23114
_4C5

(it was BBa_J23114
_4C5-1)

BBa_J23114
+
BBa_B0034
+
BBa_E1010
+
BBa_B0015 in pSB4C5.

27/08/2010

E. coli TOP10

pSB4C5

Length ok.

904 bp

constitutive promoter + RBS + mRFP1 + TT in pSB4C5

BBa_J23116
_4C5

(it was BBa_J23116
_4C5-1)

BBa_J23116
+
BBa_B0034
+
BBa_E1010
+
BBa_B0015 in pSB4C5.

30/08/2010

E. coli TOP10

pSB4C5

Length ok.

904 bp

constitutive promoter + RBS + mRFP1 + TT in pSB4C5

BBa_J23118
_4C5

(it was BBa_J23118
_4C5-1)

BBa_J23118
+
BBa_B0034
+
BBa_E1010
+
BBa_B0015 in pSB4C5.

30/08/2010

E. coli TOP10

pSB4C5

Seq ok (BMR), length ok.

904 bp

constitutive promoter + RBS + mRFP1 + TT in pSB4C5
Strain name Description Arrival date Infos Resistance Quality control Notes Store

MG1655(Seq)
(MG)

E. coli wild type strain fully sequenced

28/06/2010

link

- Genome fully sequenced Strains 2010

MC1061
(MC)

E. coli strain

28/06/2010

link

- Strain used by JC Anderson et al. for integration Strains 2010

BW25141
(BW1)

E. coli pir+ strain

28/06/2010

link

- Actually replicates the conditional replication origin R6K LC pir Strains 2010

BW25142
(BW2)

E. coli pir116 strain

28/06/2010

link

- Actually replicates the conditional replication origin R6K HC pir Strains 2010

BW23473

E. coli pir+ strain

19/07/2010

link

- LC pir Strains 2010

BW23474

E. coli pir116 strain

28/06/2010

link

- Actually replicates the conditional replication origin R6K HC pir Strains 2010

BW5328/pAH123

E. coli strain

28/06/2010

link

Amp 50 ug/ml Plasmid length ok (after digestion with SpeI).

Contains pAH123 HELPER plasmid carrying IntPhi80, low copy Amp, ori-ts 30°C

Strains 2010

BT340

E. coli strain

28/06/2010

link

Amp 100 ug/ml and Cm 34 ug/ml Plasmid relative molecular weight ok (after gel run of the undigested plasmid)

Contains pCP20 helper with yeast FRT recombinase, Amp, Cm, ori-ts 30°C

Strains 2010

DSM15372

E. coli DMS15372 strain

08/07/2010

link

Amp 100 ug/ml Length ok (after digestion with EcoRI)

Harbours the pBHR68 plasmid (contains an IPTG-inducible phaCAB operon)

Strains 2010