Team:Heidelberg/Notebook/Material

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Materials

Kits

table 1: Used kits and referring suppliers.
KitsSupplierCatalog Number
HiSpeed® Plasmid Maxi Kit (25)QIAGEN12663
CompactPrep Plasmid Maxi Kit (25)QIAGEN12763
RNeasy® Mini Kit (25)QIAGEN74106
QIAquick® Miniprep Kit (250)QIAGEN27106
QIAquick® Gel Extraction Kit (250)QIAGEN28706
QIAquick® Nucleotid Removal Kit (250)QIAGEN28306
QIAquick® PCR Purification Kit (250)QIAGEN28106
GeneMorph® II EZC Clone Domain Mutagenesis KitStratagene200552-5

Marker

table 2: Used DNA Ladder for Gel Electrophoresis.
NameSupplierRange
GeneRuler™ High Range DNA LadderFermentas10,171-48,502 bp
GeneRuler™ 1 kb Plus DNA LadderFermentas75-20,000 bp

Enzymes

table 3: Used NEB restriction enzymes
NameNumber/IDNEB buffer1NEB buffer2NEB buffer3NEB buffer4BSA
AflII2750%100%25%100%x
AgeI1100%50%10%75%
ApaI-25%50%0%100%x
AseInot75%100%not
AvrII100%100%50%100%
BamHI275%100%100%100%x
BamHI-75%100%100%100%x
BclI-50%100%100%75%
BglI-50%75%100%50%
BglII13 10% 75% 100% 10%
BsrGI- 25% 100% 10% 100%x
DpnI 6 100% 100% 75% 100%
DpnI - 100% 100% 75% 100%
EcoRI - 100% 100% 100% 100%
EcoRV - 50% 75% 100% 50% x
FseI - 100% 75% 0% 100%
HindIII- 10 50% 100% 10% 50%
HindIII-50% 100% 10% 50%
KpnI 11 100% 75% 0% 50% x
MfeI-HF™- 75% 50% 10% 100%
NarI 26 100% 75% 75% 100%
NcoI 12 100% 100% 100% 100%
NdeI - 75% 100% 75% 100%
NheI - 100% 100% 10% 100% x
NotI 14 0% 50% 100% 25%
NsiI - 10% 75% 100% 25%
PciI - 50% 75% 100% 50% x
PstI 15 75% 75% 100% 50%
SacI 16 100% 50% 10% 100%
ScaI 18 notnot100%not
SfcI 19 75% 50% 10% 100% x
SfcI-75% 50% 10% 100% x
SpeI - 75% 100% 25% 100% x
SphI - 100% 100% 50% 100%
SspI - 50% 100% 50% 50%
XbaI 23 0% 100% 75% 100% x
XbaI 56 0% 100% 75% 100% x
XhoI24 75% 100% 100% 100% x
XmaI25 25% 50% 0% 100% x

NEBuffers are color-coded (NEBuffer 1-yellow, NEBuffer 2-blue, NEBuffer 3-red, NEBuffer 4-green) and supplied as 10X

Bacteria

table 4: Used E. coli Strains.
NamePurposeReference
DH5αAmplification of PlasmidsInvitrogen
SCS110Amplification of unmethylated PlasmidsStratagene
Top10Amplification of PlasmidsInvitrogen

Media and Antibiotics

  • LB (10 g tryptone, 5 g yeast extract, 10 g sodium chloride, ad 1 L ddH2O)
table 5: Used Antibiotics.
AntibioticFinal Concentration Solvent
Ampicillin100 µg/mlddH2O
Chloramphenicol25 µg/ml100% Ethanol
Kanamycin50 µg/mlddH2O


Contents

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