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Team:MIT phage construction
From 2010.igem.org
iGEM 2010
hairy cells and polymerizing phage - construction |
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PARTS BBa_K415100 Components: RBS : M13 PVIII Explanation: This is a basic translational unit: a ribosome-binding site and the p8 gene. You can attach any promoter in front of it for expression. 
BBa_K415108 Components: RBS : M13 PIII Explanation: This is a basic translational unit: a ribosome-binding site and the p3 gene. You can attach any promoter in front of it for expression.
BBa_K415138 Components: pLac : RBS : M13 PIII Explanation: This is a basic expression unit: a promoter in front of a ribosome-binding site and the p3 gene. We used this to generate constitutive expression of p3 in cells infected with hyperphage in an attempt to rescue the wild-type, non-hairy phenotype. See the results page for an AFM image of this working. 
BBa_K415147 Components: PluxR/cI : RBS : mCherry : Term : PtetR : RBS : LuxR: Term : Plux/cI : RBS : M13 PVIII-Fos Explanation: BBa_K415148 Components: PluxR/cI : RBS : mCherry : Term : PtetR : RBS : LuxR: Term : Plux/cI : RBS : M13 PVIII-Jun Explanation: BBa_K415149 Components: PluxR/cI : RBS : mCherry : Term : PtetR : RBS : LuxR: Term : Plux/cI : RBS : M13 PVIII-ACID Explanation: BBa_K415150 Components: PluxR/cI : RBS : mCherry : Term : PtetR : RBS : LuxR: Term : Plux/cI : RBS : M13 PVIII-BASE Explanation: BBa_K415151 Components: PluxR/cI : RBS : mCherry : Term : PtetR : RBS : LuxR: Term : Plux/cI : RBS : M13 PVIII-GR1 Explanation: BBa_K415152 Components: PluxR/cI : RBS : mCherry : Term : PtetR : RBS : LuxR:Term : Plux/cI : RBS : M13 PVIII-GR2 Explanation: |
