Team:Warsaw/Stage2/Design

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• Team
• Attribution
• Sponsors
• Acknowledgemets

• Idea
• Background
• Promoters Measured
• RBSes Measured
• ExpressIt! Vectors
• Modeling

• Idea
• Background
• Design
• Results

• Idea
• Background and Design
• Results
• Gallery of microphotographs

• Idea
• Background
• Design
• Results
• Modeling

• Parts
• Labbook

• Licensing of biological parts
• Syntetic biology in Poland
• Synthetic BioLectures gallery
• iGEM survey

• About Biobrick Manager
• See Biobrick Manager

Design

The safety system we designed and measured consists of the parts specified: T7 promoter + MinC + B0032 RBS on pSB plasmid, studied in E.coli BL21 strain.

<partinfo>BBa_K299807 DeepComponents</partinfo>

We measured our construct in vivo, in the expression system, using strong T7 promoter, IPTG induced. The functionality of our safety system describes optical density of E.coli (OD) and the number of colony forming units/ ml (cfu/ml) in IPTG-induced innoculates BL21 carrying our construct. (see results)

We also performed experiment with B0034 RBS as an alternative to B0032. We designed biobrick presented below: T7 promoter + MinC + B0034 RBS on pSB plasmid, studied in E.coli BL21 strain.

<partinfo>BBa_K299808 DeepComponents</partinfo> As we know from RBS measurement data (link), B0034 is stronger than B0032. As a result, we didn’t obtain any visible bacterial colonies on agar plates. Our conslucion to this experiment is that additional regulation of MinC gene expression is very important to keep the system working.