Team:Yale/Our Project/Notebook

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lab notebook: week 1 (6/7 - 6/13)

• Monday 6/7--We successfully defend our project to our sponsors(yay!)
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• Wednesday 6/9--First day in lab!--got set up & started cultures
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Began growing two strains of E. coli--LE392 chosen as a generally healthy option, and DH5alpha chosen because Keasling et. al. used it in their thiosulfate reductase work. * Spread samples of LE392 (Grindley lab) and DH5alpha (Modis lab) on LB plates and left to incubate overnight at 37
• Thursday 6/10--inoculated liquid cultures in AM, made up different copper sulfate & LB solutions, then ran first copper growth assay in plate reader in PM w/three different ODs of each strain(4 hr. protocol)
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• Friday 6/11--Ran another growth assay with middling concentrations based on results of first, but bacteria failed to grow well--b/c allowed to overgrow?
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