Team:Yale/Our Project/Notebook

From 2010.igem.org

iGEM Yale

lab notebook: week 1 (6/7 - 6/13)

  • Monday 6/7--We successfully defend our project to our sponsors(yay!)
  • Wednesday 6/9 --First day in lab!--got set up & started cultures of two E. coli strains
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  • Thursday 6/10--inoculated liquid cultures in AM, made up different copper sulfate & LB solutions, then ran an assay measuring bacterial growth in the presence of copper, trying both strains at three different initial ODs and measuring for four hours
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  • Friday 6/11--Ran another growth assay with middling concentrations based on results of first, but bacteria failed to grow well--perhaps because allowed to overgrow prior to assay?
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