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Team:Brown/Modeling
From 2010.igem.org
Light Programmable Genetic Circuit
Modeling the Quad-state
The above model demonstrates the interaction between parts that make up the quad-state system. S1, S2, S3, and S4 are reporter molecules that represent each state, and could be replaced by any desired protein (for activation of other pathways, production of enzymes for biosynthesis, etc).
470nm illumination device
This prototype device uses two super-bright blue LEDs (470nm, 2400mcd) to illuminate cells. It will be used to drive the LOVtap promoter, which is responsive to blue light. The device is controlled by an open-source arduino microcontroller and can be custom-programmed to cycle through various light states for various amounts of time. Source code and schematics for the final device will be posted soon.
