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WRONG!!!
Team
Overview
Students
Instructors
Advisors
Institutes
Gallery
miDANCE
Project
Abstract
Introduction
Synthetic miRNA Kit
Capsid Shuffling
Homology Based
ViroBytes
miMeasure
in vivo
Study
Summary & Outlook
References
Parts
Parts
Characterization
Modeling
Introduction
miBEAT
miRockdown
miBS designer
miCrappallo
Descriptions
Notebook
Synthetic miRNA Kit
Capsid Shuffling
Homology Based
ViroBytes
miMeasure
in vivo
Study
Modeling
Material
Methods
Psychological Survey
Philosophical Reflection
Biosafety
Human Practices
Psychological Survey
Philosophical Reflection
Sponsors
Contents
1
Primer Table
2
Standart Kit Cloning Primers
3
ViroBytes primers
4
Primer Table Quick 'n' Dirty
5
Primer Table raPCR
6
Maus oligos
Primer Table
ID
Name
Sequence (5' to 3')
amplified
origin
for backbone
001
Casp8FWEcoR1
ttttgaattcatggacttcagcagaaatc
Caspase8 death gene
Caspase8
002
CMV_AgeI_fw
ttttaccggtagaatctgcttagggttagg
CMV, shRNA
pcDNA5/FRT-shRNA
pTR-UF3
003
CMVshRNApA_BglII_rv
taatagatctcagaagccatagagcccacc
CMV, shRNA
pcDNA5/FRT-shRNA
pTR-UF3
004
EGFPRVFse1
ttttggccggcccttgtacagctcgtccatg
EGFP
GFP
005
ElenasFirstSyntheticRNA_F
aatatgtctaaactattatttatgccaaccagccaatctagctactgctaggc
part 2 Elenas first synthetic shRNA
miRsAg
pC-DNA5
006
ElenasFirstSyntheticRNA_R
aataatagtttagacatatttatgccagccagccagaccagctctgctaagg
part 3 Elenas first synthetic shRNA
miRsAg
pC-DNA5
007
ElenasSecondSyntheticRNA_F
gacatgtctaaactattgtcttcggtagcgtcgtagactagctactgctaggc
part 2 Elenas second synthetic shRNA
miRsAg
pC-DNA5
008
ElenasSecondSyntheticRNA_R
gacaatagtttagacatgtcttcggtatcgtcgtatcccagctctgctaagg
part 3 Elenas second synthetic shRNA
miRsAg
pC-DNA5
009
ElenasThirdSyntheticRNA_F
tatttgtctaaactataataattcgcggctggcctgactagctactgctaggc
part 2 Elenas third synthetic shRNA
miRsAg
pC-DNA5
010
ElenasThirdSyntheticRNA_R
tattatagtttagacaaataattcgcgtctggccttcccagctctgctaagg
part 3 Elenas third synthetic shRNA
miRsAg
pC-DNA5
011
ElenasFifthSyntheticRNA_F
tatttgtctaaactataataattcgcggctggcctgactagctactgctaggc
part 2 Elenas fifth synthetic shRNA
miRsAg
pC-DNA5
012
ElenasFifthSyntheticRNA_R
atctatagtttagacaagatgaaacgccgagttaacgccagctctgctaagg
part 3 Elenas fifth synthetic shRNA
miRsAg
pC-DNA5
013
ElenasSixthSyntheticRNA_F
ttattgtctaaactatataactgccgtaactccaaatctagctactgctaggc
part 2 Elenas sixth synthetic shRNA
miRsAg
pC-DNA5
014
ElenasSixthSyntheticRNA_R
ttatatagtttagacaataactgccgtcactccaacgccagctctgctaagg
part 3 Elenas sixth synthetic shRNA
miRsAg
pC-DNA5
015
ElenasSeventhSyntheticRNA_F
atcttgtctaaactatagataactgccatcactccccctagctactgctaggc
part 2 Elenas seventh synthetic shRNA
miRsAg
pC-DNA5
016
ElenasSeventhSyntheticRNA_R
atctatagtttagacaagataactgccgtcactccaaccagctctgctaagg
part 3 Elenas seventh synthetic shRNA
miRsAg
pC-DNA5
017
ElenasEighthSyntheticRNA_F
tacatgtctaaactattgtagtcggttcatgcagcccctagctactgctaggc
part 2 Elenas eighth synthetic shRNA
miRsAg
pC-DNA5
018
ElenasEighthSyntheticRNA_R
tacaatagtttagacatgtagtcggtttatgcagcaaccagctctgctaagg
part 3 Elenas eighth synthetic shRNA
miRsAg
pC-DNA5
019
ElenasNinethSyntheticRNA_F
aaattgtctaaactatatttgatccagagatacagaactagctactgctaggc
part 2 Elenas nineth synthetic shRNA
miRsAg
pC-DNA5
020
ElenasNinethSyntheticRNA_R
aaatatagtttagacaatttgatccagcgatacagcgccagctctgctaagg
part 3 Elenas nineth synthetic shRNA
miRsAg
pC-DNA5
021
ElenasTenthSyntheticRNA_F
attatgtctaaactattaatatcggtgaccgtggtacctagctactgctaggc
part 2 Elenas tenth synthetic shRNA
miRsAg
pC-DNA5
022
ElenasTenthSyntheticRNA_R
attaatagtttagacataatatcggtggccgtggtgtccagctctgctaagg
part 3 Elenas tenth synthetic shRNA
miRsAg
pC-DNA5
023
FADDFWEcoR1
ttttgaattcatgggcggtaggcgtgtacg
FADD
024
gE1A-Fse1_rev
tttttccggccggttatggcctggggcctttaca
E1A rescue gene
025
Hcrfor_HindIII_AgeI_TL
gagtcaagcttaccggttggaggtgaagttaacaccttcgtg
part 1 shRNA against everything; cutting site: HindIII, AgeI
miRsAg
pC-DNA5
026
Hcrrev_ApaI_SalI_TL
ctcgagggcccgtcgacaagcaaacgatgccaagacatttatcg
part 4 shRNA against everything; cutting site: ApaI, SalI
miRsAg
pC-DNA5
027
HSVluc_norm_BglII_fw
tttttagatcttgcaggagcttcagggagtg
HSV-TK,
Firefly
Luciferase
psiCHECK2
pTR-UF3
028
HSVluc_norm_BglII_rv
ttttagatctggttccgcgcacatttccc
HSV-TK,
Firefly
Luciferase
psiCHECK2
pTR-UF3
029
Kif11_PLK1_shRNA-hcrfor:ecoRI
gagtcgaattctggaggtgaagttaacaccttcgtg
part 1 shRNA against everything; cutting site: ecoRI
miRsAg
pTR-UF3
030
Kif11&PLK1_shRNA_hcrrev:XbaI
ctcgaagatctaagcaaacgatgccaagacatttatcg
part 4 shRNA against everything; cutting site: XbaI
miRsAg
pTR-UF3
031
Kif11_shRNA_forward
actctgtctaaactatgagtacattaaacaattcctattagctactgctaggc
part 2 shRNA against Kif11
miRsAg
pTR-UF3
032
Kif11_shRNA_reverse
actcatagtttagacagagtacattaatcaattccattcagctctgctaagg
part3 shRNA against Kif11
miRsAg
pTR-UF3
033
PLK1_shRNA_forward
atgatgtctaaactattcattaagcagatcgttaaccgtagctactgctaggc
part2 shRNA against PLK1
miRsAg
pTR-UF3
034
PLK1_shRNA_reverse
atgaatagtttagacatcattaagcagctcgttaatggcagctctgctaagg
part3 shRNA against PLK1
miRsAg
pTR-UF3
035
pTR-UF3_Nsp1_for
ctattacgccagctggatgcatcccagctgc
for pTR-UF3 backbone Pst1->Nsi1 mutation
036
pTR-UF3_Nsp1_rev
gcagctgggatgcatccagctggcgtaatag
for pTR-UF3 backbone Pst1->Nsi1 mutation
037
pTR-UF3_PstI-NsiI_for
gctattacgccagctggatgcatcccagctgcattaatgaatcgg
mutagenesis of PstI to NsiI
pTR-UF3
pTR-UF3
038
pTR-UF3_PstI-NsiI_rev
ccgattcattaatgcagctgggatgcatccagctggcgtaatagc
mutagenesis of PstI to NsiI
pTR-UF3
pTR-UF3
039
pTR-UF3_PstI-NsiI_shifted_rev
cattaatgcagctgggatgcatccagctggcgtaatagcgaagag
mutagenesis of PstI to NsiI
pTR-UF3
pTR-UF3
040
pTRUF3_seq_fwrv
caactccatcactaggggttcct
sequencing purpose
pTR-UF3
pTR-UF3
041
SCLpsv40_FW
ctcaattagtcagcaaccatagtccc
FWprimer for testing stable integration of shRNA into T-Rex
T-REx cells
042
SCLpsv40_FW
gcaaagtgccgataaacataacgatct
RVprimer for testing stable integration of shRNA into T-Rex
T-REx cells
043
Sequencing primer_pTR-UF3 mutated
ctaaatcggaaccctaaagggagcccccg
sequencing of mutagenesis of PstI to NsiI
pTR-UF3
pTR-UF3
044
Sv40lucmcs_AgeI_fw
ttttaccggtgtggaatgtgtgtcagttag
SV40,
Renilla
Luciferase, MCS
psiCHECK2
pTR-UF3
045
Sv40lucmcs_BglII_rv
tattagatctccgcgtcagacaaaccctaac
SV40,
Renilla
Luciferase, MCS
psiCHECK2
pTR-UF3
046
tBidGFPRVFse1
tttttggccggccttacatgtacagctcg
tBid death gene
pcherryBidGFP
047
tBidFWEcoR1
tttttgaattcaaccgcagcagccactc
tBid death gene
pcherryBidGFP
048
tBidRVFse1
tttttccggccgggtccatcccatttctg
tBid death gene
pcherryBidGFP
049
TetO2FW
ggggacaacttttctatacaaagttgtccctatcagtgatagagatctc
TetO8
synthesized tetO8
Gateway entry vector
Standart Kit Cloning Primers
ID
Name
Sequence (5' to 3')
amplified
origin
for backbone
D001
backbone_AvrII_fw
ttttcctaggtagcggcgcattaagcgcgg
-
-
-
D002
backbone_AvrII_rev
ttttcctaggcttgttgtagcttaaattttg
-
-
-
D003
backbone_ClaI_fw
ttttatcgattagcggcgcattaagcgcgg
-
-
-
D004
backbone_XhoI_rev
ttttctcgagcttgttgtagcttaaattttg
-
-
-
D005
BBB_Oligo_XhoI
ccggActgcagcggccgcgcgctagcacactagtgcggccgcgaattCa
-
-
-
D006
BBB_Oligo_XmaI
tcgatgaattcgcggccgcactagtgtgctagcgcgcggccgctgcagt
-
-
-
D007
BBB_Oligo_XmaI
tcgatgaattcgcggccgcactagtgtgctagcgcgcggccgctgcagt
-
-
-
D008
BBB_suffix_reverse
aaaactgcagcggccgcgc
-
-
-
D009
BGH_pA_BamHI_rc_fw
ttttgaattcgcggccgcactagtggatccccatagagcccaccgcatccc
-
-
-
D010
BGH_pA_fw
ttttgaattcgcggccgcactagttttaaacccgctgatcagcc
-
-
-
D011
BGH_pA_rev
ttttctgcagcggccgcgctagcccatagagcccaccgcatccc
-
-
-
D012
BGH_pA_rc_rev
ttttctgcagcggccgcgctagctttaaacccgctgatcagcc
-
-
-
D013
biCMV_BBB_fw
ttttgaattcgcggccgcactagtgcgatctgacggttcactaaacg
-
-
-
D014
biCMV_BBB_fw
ttttgaattcgcggccgcactagtgcgatctgacggttcactaaacg
-
-
-
D015
biCMV_BBB_rev
ttttctgcagcggccgcgcgctagcacagcggatctgacggttcac
-
-
-
D016
biCMV_rc_BBB_fw
ttttgaattcgcggccgcactagtagcggatctgacggttcac
-
-
-
D017
biCMV_rc_BBB_rev
ttttctgcagcggccgcgctagcgcgatctgacggttcac
-
-
-
D018
CMV_BBB_BamHI_fw
gaattcgcggccgcactagtggatcccgatgtacgggccagatatacg
-
-
-
D019
CMV_BBB_fw
ttttgaattcgcggccgcactagtcgatgtacgggccagatatacg
-
-
-
D020
CMV_BBB_rev
ttttctgcagcggccgcgcgctagcacatttcgataagccagtaagc
-
-
-
D021
CMV_Teto2_BBB_fw
tttt gaattcgcggccgcactagtgttgacattgattattgtctag
-
-
-
D022
CMV_TetO2_BBB_rev
tttt ctgcagcggccgcgcgctagcaccggaggctggatcggtc
-
-
-
D023
CMV_rc_BBB_fw
ttttgaattcgcggccgcactagtatttcgataagccagtaagc
-
-
-
D024
CMV_rc_BBB_rev
ttttctgcagcggccgcgctagccgatgtacgggccagatatacg
-
-
-
D025
FRT_rev
ctgcagcggccgcgctagcccaaggaagttcctatactttc
-
-
-
D026
FRT_rev
gaattcgcggccgcactagtttctcgccacgttcgccggc
-
-
-
D027
hRluc_ter_BBB_fw
ttttgaattcgcggccgcactagtatggcttccaaggtgtacgac
-
-
-
D028
hRluc_ter_BBB_rev
ttttctgcagcggccgcgctagcttactgctcgttcttcagcacgc
-
-
-
D029
hsa-mir-122_BBB(perf)
ttttgaattcgcggccgcactagtcaaacaccattgtcacactccagctagcgcggccgctgcagtttt
-
-
-
D030
hsa-mir-122_BBB(rand9-12)
ttttgaattcgcggccgcactagtcaaacaccatnnnnacactccagctagcgcggccgctgcagtttt
-
-
-
D031
hsa-mir-122_BBB(rand9-22)
ttttgaattcgcggccgcactagtnnnnnnnnnnnnnnacactccagctagcgcggccgctgcagtttt
-
-
-
D032
hsa-mir-122_Sgf_Not(perf)
ttttgcgatcgccaaacaccattgtcacactccagcggccgctatgtcacaact
-
-
-
D033
hsa-mir-122_Sgf_Not(rand9-12)
ttttgcgatcgccaaacaccatnnnnacactccagcggccgctatgtcacaact
-
-
-
D034
hsa-mir-122_Sgf_Not(rand9-22)
ttttgcgatcgcnnnnnnnnnnnnnnacactccagcggccgctatgtcacaact
-
-
-
D035
hsa-mir-375_BBB(perf)
ttttgaattcgcggccgcactagttcacgcgagccgaacgaacaaagctagcgcggccgctgcagtttt
-
-
-
D036
hsa-mir-375_BBB(rand9-12)
ttttgaattcgcggccgcactagttcacgcgagcnnnncgaacaaagctagcgcggccgctgcagtttt
-
-
-
D037
hsa-mir-375_BBB(rand9-22)
ttttgaattcgcggccgcactagtnnnnnnnnnnnnnncgaacaaagctagcgcggccgctgcagtttt
-
-
-
D038
hsa-mir-375_Sgf_Not(perf)
ttttgcgatcgctcacgcgagccgaacgaacaaagcggccgctatgtcacaact
-
-
-
D039
hsa-mir-375_Sgf_Not(rand9-12)
ttttgcgatcgctcacgcgagcnnnncgaacaaagcggccgctatgtcacaact
-
-
-
D040
hsa-mir-375_Sgf_Not(rand9-22)
ttttgcgatcgcnnnnnnnnnnnnnncgaacaaagcggccgctatgtcacaact
-
-
-
D041
hsa-mir-376a_BBB(perf)
ttttgaattcgcggccgcactagtacgtggattttcctctatgatgctagcgcggccgctgcagtttt
-
-
-
D042
hsa-mir-376a_BBB(rand9-12)
ttttgaattcgcggccgcactagtacgtggattnnnntctatgatgctagcgcggccgctgcagtttt
-
-
-
D043
hsa-mir-376a_BBB(rand9-22)
ttttgaattcgcggccgcactagtnnnnnnnnnnnnntctatgatgctagcgcggccgctgcagtttt
-
-
-
D044
hsa-mir-376a_Sgf_Not(perf)
ttttgcgatcgcacgtggattttcctctatgatgcggccgctatgtcacaact
-
-
-
D045
hsa-mir-376a_Sgf_Not(rand9-12)
ttttgcgatcgcacgtggattnnnntctatgatgcggccgctatgtcacaact
-
-
-
D046
hsa-mir-376a_Sgf_Not(rand9-22)
ttttgcgatcgcnnnnnnnnnnnnntctatgatgcggccgctatgtcacaact
-
-
-
D047
igem20100723_01
tgccacctgacgtctaagaa
-
-
-
D048
igem20100723_02
attaccgcctttgagtgagc
-
-
-
D049
Luc2_ter_BamHI_BBB_rev
ttttctgcagcggccgcgctagcggatcctaccacatttgtagaggttttac
-
-
-
D050
Luc2_ter_BamHI_BBB_rev
ttttctgcagcggccgcgctagcggatcctaccacatttgtagaggttttac
-
-
-
D051
Luc2_ter_BBB_fw
ttttgaattcgcggccgcactagtgccaccatggaagatgcc
-
-
-
D052
mir122_miMeasure_screen_fev
ccgcgctagctggagtgt
-
-
-
D053
Oligo_Xba_mut_fw
ctagcctcgagttctgaccgccccgggg
-
-
-
D054
Oligo_Xba_mut_rev
ctagccccggggcggtcagaactcgagg
-
-
-
D055
pSMB_miMeasure_Screen_fw
gcacaagctggagtacaactac
-
-
-
D056
pSMB_miMeasure_Screen_rev
ggtttcccgactggaaagcg
-
-
-
D057
RSV_fw
ttttgaattcgcggccgcactagtcaattctcatgtttgacagc
-
-
-
D058
RSV_rev
ttttctgcagcggccgcgctagccagcttggaggtgcacacc
-
-
-
D059
RSV_rc_fw
ttttgaattcgcggccgcactagtcagcttggaggtgcacacc
-
-
-
D060
RSV_rc_rev
ttttctgcagcggccgcgctagccaattctcatgtttgacagc
-
-
-
D061
RSV_fw_midcomp
cgaaccactgaataccgcattgcag
-
-
-
D062
RSV_rev_midcomp
ctgcaatgcggtattcagtggttcg
-
-
-
D063
SB-prep-3P
gccgctgcagtccggcaaaaaaacg
-
-
-
D064
SB-prep-2Ea
atgaattccagaaatcatccttagcg
-
-
-
D065
second_strand_notI
agttgtgacatagcggccgc
-
-
-
D066
shRNA-6th-bs(custom)_BBB
ttttgaattcgcggccgcactagtnnnnnnnnnnnnnncgtcaatagctagcgcggccgctgcagtttt
-
-
-
D067
shRNA-7th-bs(custom)_BBB
ttttgaattcgcggccgcactagtnnnnnnnnnnnnnntcaatagagctagcgcggccgctgcagtttt
-
-
-
D068
shRNA-8th-bs(custom)_BBB
ttttgaattcgcggccgcactagtnnnnnnnnnnnnnngctgatgtgctagcgcggccgctgcagtttt
-
-
-
D069
shRNA-9th-bs(custom)_BBB
ttttgaattcgcggccgcactagtnnnnnnnnnnnnnnctagtttagctagcgcggccgctgcagtttt
-
-
-
D070
shRNA-10th-bs(custom)_BBB
ttttgaattcgcggccgcactagtnnnnnnnnnnnnnngctataatgctagcgcggccgctgcagtttt
-
-
-
D071
shRNA-6th-bs(perf)_BBB
ttttgaattcgcggccgcactagtcgcaacctcactgccgtcaatagctagcgcggccgctgcagtttt
-
-
-
D072
shRNA-7th-bs(perf)_BBB
ttttgaattcgcggccgcactagtcaacctcactgccgtcaatagagctagcgcggccgctgcagtttt
-
-
-
D073
shRNA-8th-bs(perf)_BBB
ttttgaattcgcggccgcactagtcaacgacgtatttggctgatgtgctagcgcggccgctgcagtttt
-
-
-
D074
shRNA-9th-bs(perf)_BBB
ttttgaattcgcggccgcactagtcgcgacatagcgacctagtttagctagcgcggccgctgcagtttt
-
-
-
D075
shRNA-10th-bs(perf)_BBB
ttttgaattcgcggccgcactagtctgtggtgccggtggctataatgctagcgcggccgctgcagtttt
-
-
-
D076
shRNA-6th-bs(rand9-12)_BBB
gcgatcgccgcaacctcannnncgtcaatagcggccgctatgtcacaact
-
-
-
D077
shRNA-7th-bs(rand9-12)_BBB
ttttgaattcgcggccgcactagtcaacctcactnnnntcaatagagctagcgcggccgctgcagtttt
-
-
-
D078
shRNA-8th-bs(rand9-12)_BBB
ttttgaattcgcggccgcactagtcaacgacgtannnngctgatgtgctagcgcggccgctgcagtttt
-
-
-
D079
shRNA-9th-bs(rand9-12)_BBB
ttttgaattcgcggccgcactagtcgcgacatagnnnnctagtttagctagcgcggccgctgcagtttt
-
-
-
D080
shRNA-10th-bs(rand9-12)_BBB
ttttgaattcgcggccgcactagtctgtggtgccnnnngctataatgctagcgcggccgctgcagtttt
-
-
-
D081
shRNA-6th-bs(custom)_SgfI_NotI
gcgatcgcnnnnnnnnnnnnnncgtcaatagcggccgctatgtcacaact
-
-
-
D082
shRNA-7th-bs(custom)_SgfI_NotI
gcgatcgcnnnnnnnnnnnnnntcaatagagcggccgctatgtcacaact
-
-
-
D083
shRNA-8th-bs(custom)_SgfI_NotI
gcgatcgcnnnnnnnnnnnnnngctgatgtgcggccgctatgtcacaact
-
-
-
D084
shRNA-9th-bs(custom)_SgfI_NotI
gcgatcgcnnnnnnnnnnnnnnctagtttagcggccgctatgtcacaact
-
-
-
D085
shRNA-10th-bs(custom)_SgfI_NotI
gcgatcgcnnnnnnnnnnnnnngctataatgcggccgctatgtcacaact
-
-
-
D086
shRNA-6th-bs(perf)_SgfI_NotI
gcgatcgccgcaacctcactgccgtcaatagcggccgctatgtcacaact
-
-
-
D087
shRNA-7th-bs(perf)_SgfI_NotI
gcgatcgccaacctcactgccgtcaatagagcggccgctatgtcacaact
-
-
-
D088
shRNA-8th-bs(perf)_SgfI_NotI
gcgatcgccaacgacgtatttggctgatgtgcggccgctatgtcacaact
-
-
-
D089
shRNA-9th-bs(perf)_SgfI_NotI
gcgatcgccgcgacatagcgacctagtttagcggccgctatgtcacaact
-
-
-
D090
shRNA-10th-bs(perf)_SgfI_NotI
gcgatcgcctgtggtgccggtggctataatgcggccgctatgtcacaact
-
-
-
D091
shRNA-6th-bs(rand9-12)_SgfI_NotI
gcgatcgccgcaacctcannnncgtcaatagcggccgctatgtcacaact
-
-
-
D092
shRNA-7th-bs(rand9-12)_SgfI_NotI
gcgatcgccaacctcactnnnntcaatagagcggccgctatgtcacaact
-
-
-
D093
shRNA-8th-bs(rand9-12)_SgfI_NotI
gcgatcgccaacgacgtannnngctgatgtgcggccgctatgtcacaact
-
-
-
D094
shRNA-9th-bs(rand9-12)_SgfI_NotI
gcgatcgccgcgacatagnnnnctagtttagcggccgctatgtcacaact
-
-
-
D095
shRNA-10th-bs(rand9-12)_SgfI_NotI
gcgatcgcctgtggtgccnnnngctataatgcggccgctatgtcacaact
-
-
-
D096
shRNA_AflII_BBB_fw
ttttctgcagcggccgcgcgctagccttaagtggaggtgaagttaacaccttcgtg
-
-
-
D097
shRNA_HindIII_BBB_rev
ttttgaattcgcggccgcactagtaagcttaagcaaacgatgccaagacatttatcg
-
-
-
D098
SV40_BamHI_BBB_fw
ctgcagcggccgcgctagcggatccaagctttttgcaaaagcctagg
-
-
-
D099
SV40_BBB_rev
ttttctgcagcggccgcgctagcaagctttttgcaaaagcctaggc
-
-
-
D100
SV40_rc_BBB_fw
ttttgaattcgcggccgcactagtaagctttttgcaaaagcc
-
-
-
D101
SV40_rc_BBB_rev
ttttctgcagcggccgcgctagcgcgcagcaccatggcctg
-
-
-
D102
SV40_term_fw
ttttgaattcgcggccgcactagtcagacatgataagatacattg
-
-
-
D103
SV40_Xho_Xma_BBB_fw
gaattcgcggccgcactagtctcgagtttctcccggggcgcagcaccatggcctg
-
-
-
D104
TetRepressor (from pcDNA6)fw
ttttgaattcgcggccgcactagtgccaccatgtctagattagataaaag
-
-
-
D105
TetRepressor (from pcDNA6)rew
ttttctgcagcggccgcgctagcttaataagatctaaattcccgcgatccgc
-
-
-
ViroBytes primers
ID
Name
Sequence (5' to 3')
amplified fragment
direction
cap gene of AAV
R001
Anchor12367891012
tctctctctctctctaagcttgtctgagtgactagcattcgttaattaacTACCG
anchor
-
R002
Anchor4
tctctctctctctctaagcttgtctgagtgactagcattcgttaattaactactg
anchor
-
R003
Anchor5
tctctctctctctctaagcttgtctgagtgactagcattcgttaattaactacag
anchor
-
R004
Anchor_comp_all
gttaattaacgaatgctagtcactcagacaagctt
anchor
-
R005
F1_for_cap12678910
gctacggtctcaatggctgccgatggttatcttccag
1
forward
1,2,6,7,8,9,10
R006
F1_for_cap5
gctacggtctcaatggctgccgatggttatcttccag
1
forward
5
R007
F1_rev_cap2
gctacggtctcgctcctgaaactcggcgtcggcgtgg
1
reverse
2
R008
F1_rev_cap5
gctacggtctctctcctgaaactcggcgtccgcgtgg
1
reverse
5
R009
F1_rev_cap9
gctacggtctcgctcctggaactcggcgtcggcgtgg
1
reverse
9
R010
F1_rev_cap168
gctacggtctcgctcctgaaactcggcgtcggcgtgg
1
reverse
1,6,8
R011
F2_for_cap2
gctacggtctctggagcgccttaaagaagatacgtcttttggggg
2
forward
2
R012
F2_for_cap5
gctacggtctctggagaagctcgccgacgacacatccttcggggg
2
forward
5
R013
F2_for_cap9
gctacggtctctggagcggctcaaagaagatacgtcttttggggg
2
forward
9
R014
F2_for_cap168
gctacggtctctggagcgtctgcaagaagatacgtcttttggggg
2
forward
1,6,8
R015
F2_rev_cap2
gctacggtctcgtgtcgcccatccatgtggaatcgcaatgc
2
reverse
2
R016
F2_rev_cap5
gctacggtctcgtgtcccccatccacgtggaatcgcaatgc
2
reverse
5
R017
F2_rev_cap9
gctacggtctcgtgtcccccagccattgggaatcgcaatgc
2
reverse
9
R018
F2_rev_cap168
gctacggtctcgtgtcgcccagccatgtggaatcgcaatgc
2
reverse
1,6,8
R019
F3_for_cap5
gctacggtctccgacagagtcatcaccaccagcacccg
3
forward
5
R020
F3_for_cap9
gctacggtctccgacagagtcatcaccaccagcacccg
3
forward
9
R021
F3_for_cap1268
gctacggtctccgacagagtcatcaccaccagcacccg
3
forward
1,2,6,8
R022
F3_rev_cap1
gctacggtctcaggttattagcgatggttgtgacgccatcattcgtc
3
reverse
1
R023
F3_rev_cap2
gctacggtctcaggttattggcaatcgtcgtcgtaccgtcattctgc
3
reverse
2
R024
F3_rev_cap5
gctacggtctcaggttgttggcgatggtggtggtggagtcctgcac
3
reverse
5
R025
F3_rev_cap6
gctacggtctcaggttattagcgatggtcgtgacgccatcattcgtc
3
reverse
6
R026
F3_rev_cap8
gctacggtctcaggttattggcgatggtcttggtgccttcattctgc
3
reverse
8
R027
F3_rev_cap9
gctacggtctcaggttattggcgatggtcttgactccattgttgtcc
3
reverse
9
R028
F4_for_cap2
gctacggtctctaaccttaccagcacggttcaggtgtttactgactc
4
forward
2
R029
F4_for_cap5
gctacggtctccaacctcacctccaccgtccaagtgtttacggacga
4
forward
5
R030
F4_for_cap8
gctacggtctctaacctcaccagcaccatccaggtgtttacggactc
4
forward
8
R031
F4_for_cap9
gctacggtctctaaccttaccagcacggtccaggtcttcacggactc
4
forward
9
R032
F4_for_cap16
gctacggtctctaaccttaccagcacggttcaagtcttctcggactc
4
forward
1,6
R033
F4_reverse_cap1
gctacggtctcagctgctgtggaaaggcacttcctcaaaggtg
4
reverse
1
R034
F4_reverse_cap2
gctacggtctcagctgctgtggaaaggaacgtcctcaaaagtg
4
reverse
2
R035
F4_reverse_cap5
gctacggtctcagctggagtggaagggcacctcctcaaagttg
4
reverse
5
R036
F4_reverse_cap9
gctacggtctcagctgctatggaaaggtacgttctcaaactcg
4
reverse
9
R037
F4_reverse_cap68
gctacggtctcagctgctgtggaaaggcacgtcctcgaaggtg
4
reverse
6,8
R038
F5_for_cap2
gctacggtctcgcagctacgctcacagccagagtctggaccgtc
5
forward
2
R039
F5_for_cap5
gctacggtctcccagcttcgctcccagtcagaacctgttcaagc
5
forward
5
R040
F5_for_cap8
gctacggtctcgcagctacgcccacagccagagcttggaccggc
5
forward
8
R041
F5_for_cap9
gctacggtctcgcagctacgctcacagccaaagcctggaccgac
5
forward
9
R042
F5_for_cap16
gctacggtctcgcagctacgcgcacagccagagcctggaccggc
5
forward
1,6
R043
F5_rev_cap2
gctacggtctccagttcctagactggtcccgaatgtcactcg
5
reverse
2
R044
F5_rev_cap5
gctacggtctccagtttttgtaggtgttggcgtatctcccgg
5
reverse
5
R045
F5_rev_cap8
gctacggtctccagttctttgcctgattggccattgtattag
5
reverse
8
R046
F5_rev_cap9
gctacggtctctagtttcttccctggacagccatgttgctgg
5
reverse
9
R047
F5_rev_cap16
gctacggtctccagtttttgggctgaacagacatgccagctg
5
reverse
1,6
R048
F6_for_cap2
gctacggtctcgaactggcttcctggaccctgttaccgccagc
6
forward
2
R049
F6_for_cap5
gctacggtctcaaactggttcccggggcccatgggccgaaccc
6
forward
5
R050
F6_for_cap8
gctacggtctcgaactggctgccaggaccctgttaccgccaac
6
forward
8
R051
F6_for_cap9
gctacggtctcaaactacatacctggacccagctaccgacaac
6
forward
9
R052
F6_for_cap16
gctacggtctcaaactggctacctggaccctgttatcggcagc
6
forward
1,6
R053
F6_rev_cap1
gctacggtctcgccacagggttagtggctttaatttcctcttcgtctg
6
reverse
1
R054
F6_rev_cap2
gctacggtctcgccacgggattggttgtcctgatttcctcttcgtctg
6
reverse
2
R055
F6_rev_cap5
gctacggtctcgccacgcggttcaccggctgcgtctcgctctcgctgg
6
reverse
5
R056
F6_rev_cap6
gctacggtctcgccacggggttagtggctttgatttcctcttcgtctg
6
reverse
6
R057
F6_rev_cap8
gctacggtctcgccacagggttagtggttttgatttcttctcgctgg
6
reverse
8
R058
F6_rev_cap9
gctacggtctcgccaccgggttagtagttttaatttcttcttcgttggttatc
6
reverse
9
R059
F7_for_cap1
gctacggtctctgtggccaccgaaagatttgggaccgtggcagtcaatttc
7
forward
1
R060
F7_for_cap2
gctacggtctccgtggctacggagcagtatggttctgtatctaccaacctc
7
forward
2
R061
F7_for_cap5
gctacggtctccgtggcgtacaacgtcggcgggcagatg
7
forward
5
R062
F7_for_cap6
gctacggtctccgtggccaccgaaagatttgggactgtggcagtcaatctc
7
forward
6
R063
F7_for_cap8
gctacggtctctgtggctacagaggaatacggtatcgtggcagataacttg
7
forward
8
R064
F7_for_cap9
gctacggtctcggtagcaacggagtcctatggacaagtggccacaaaccac
7
forward
9
R065
F7_rev_cap1
gctacggtctcggtgatgaatgaagcaaactttgtagctgaaaac
7
reverse
1
R066
F7_rev_cap2
gctacggtctctgtgatgaaggaagcaaactttgccgcactgaag
7
reverse
2
R067
F7_rev_cap5
gctacggtctcggtgatgaagctgctgacgggcacgtccgagaag
7
reverse
5
R068
F7_rev_cap6
gctacggtctcggtgatgaatgaagcaaactttgtagccgaaaac
7
reverse
6
R069
F7_rev_cap8
gctacggtctccgtgatgaaagagttcagctttgactggttgaag
7
reverse
8
R070
F7_rev_cap9
gctacggtctcggtgatgaaagagttcagcttgtccttgttgaag
7
reverse
9
R071
F8_for_cap1
gctacggtctcatcacccaatactccacaggacaagtgagtgtgg
8
forward
1
R072
F8_for_cap2
gctacggtctcatcacacagtactccacgggacaggtcagcgtgg
8
forward
2
R073
F8_for_cap5
gctacggtctcatcacccagtacagcaccgggcaggtcaccgtgg
8
forward
5
R074
F8_for_cap6
gctacggtctcatcacccagtattccacaggacaagtgagcgtgg
8
forward
6
R075
F8_for_cap8
gctacggtctcatcacgcaatacagcaccggacaggtcagcgtgg
8
forward
8
R076
F8_for_cap9
gctacggtctcatcacccagtattctactggccaagtcagcgtgg
8
forward
9
R077
F8_rev_cap1
gctacggcgcgccttacaggggacgggtaaggtaacgggtgcc
8
reverse
1
R078
F8_rev_cap2
gctacggcgcgccttacagattacgagtcaggtatctggtgccaatggg
8
reverse
2
R079
F8_rev_cap5
gctacggcgcgccttaaaggggtcgggtaaggtatcgggttccgatagg
8
reverse
5
R080
F8_rev_cap6
gctacggcgcgccttacaggggacgggtgaggtaacgggtgcc
8
reverse
6
R081
F8_rev_cap8
gctacggcgcgccttacagattacgggtgaggtaacgggtgccaatggg
8
reverse
8
R082
F8_rev_cap9
gctacggcgcgccttacagattacgagtcaggtatctggtgccaatggg
8
reverse
9
R083
Virbyte_for
gtctgagtgactagcattcggtctgagtgactagcattcg
-
forward
R084
Virobyte_rev
gctacggcgcgcctta
-
reverse
Primer Table Quick 'n' Dirty
ID
Name
Sequence (5' to 3')
amplified
origin
for backbone
L001
CMV_AgeI_fw
ttttaccggtagaatctgcttagggttagg
for use with L11
from pcDNA5+miRNA
to tuning construct-pTR-UF3 KpnI/SalI
L002
CMVshRNApA_KpnI_rv
taatggtacccagaagccatagagcccacc
-
from pcDNA5+miRNA
to tuning construct-pTR-UF3 KpnI/SalI
L003
HSVluc_norm_BglII_fw
tttttagatcttgcaggagcttcagggagtg
for use with L8
from PsiCheck2
to pTR-UF3 BglII
L004
HSVluc_norm_BglII_rv
ttttagatctggttccgcgcacatttccc
for use with L7
from PsiCheck2
to pTR-UF3 BglII
L005
HSVluc_norm_KpnI_fw
tttttggtagcaggagcttcagggagtg
for use with L16
from PsiCheck2
to pTR-UF3 KpnI/SalI
L006
HSVluc_norm_SalI_rv
ttttgtcgacggttccgcgcacatttccc
for use with L15
from PsiCheck2
to pTR-UF3 KpnI/SalI
L007
Sv40lucmcs_AgeI_fw
ttttaccggtgtggaatgtgtgtcagttag
for use with L13
from PsiCheck2
to tuning construct-pTR-UF3 KpnI/SalI
L008
SV40luc_SalI_rv
tattgtcgacccgcgtcagacaaaccctaac
for use with L1
from PsiCheck2
to tuning construct-pTR-UF3 KpnI/SalI
L009
TetO2_StuI_NheI
ttttaggccttccctatcagtgatagagatctccctatcagtgatagagagctagctaac
Tet Repressor
Oligo
to final tuning construct
L010
TetR_BspEI_fw
tttttccggaggcgaattgatatgtctagattag
for use with L14
from pcDNA6/TR
pTR-UF3 BspEI/NotI
L011
TetR_SgfI_NotI_rv
ttttgcggccgctggcgatcgctaataagatctgaattcccgggatc
for use with L12
from pcDNA6/TR
to pTR-UF3 BspEI/NotI
Primer Table raPCR
ID
Name
Sequence (5' to 3')
target
Assembly Site
ra001
psicheck2_MCS_min_200_fw
cagcgacgatctgcctaa
sequencing fw
sequencing Primer for binding-sites in pSiCheck vector
ra002
psicheck2_MCS_plu_200_rv
gtggccaccaagaccaaa
sequencing rv
sequencing Primer for binding-sites in pSiCheck vector
ra003
raPCR_AS13-hsa-mir-122
cactgaatccaactgcaaacaccattgtcacactccagcatacatggactgc
hsa-mir-122
13 bp
ra004
raPCR_AS13-has-mir122(ran9-12)
cactgaatccaactgcaaacaccatnnnnacactccagcatacatggactgc
has-mir122 (ran9-12)
13 bp
randomised nucleotides 9-12
ra005
raPCR_AS13-hsa-mir-320b
cactgaatccaactgttgccctctcaacccagcttttgcatacatggactgc
hsa-mir-320b
13 bp
ra006
raPCR_AS13-has-mir-221
cactgaatccaactggaaacccagcagacaatgtagctgcatacatggactgc
has-mir-221
13 bp
ra007
raPCR_AS13-has-mir-221(ran9-12
cactgaatccaactggaaacccagcannnnatgtagctgcatacatggactgc
has-mir-221 (ran9-12)
13 bp
randomised nucleotides 9-12
ra008
raPCR_AS13-has-mir-1179
cactgaatccaactgccaaccaatgaaagaatgcttgcatacatggactgc
has-mir-1179
13 bp
ra009
raPCR_AS13-has-mir-1179ran9-12
cactgaatccaactgccaaccaatnnnngaatgcttgcatacatggactgc
has-mir-1179 (ran9-12)
13 bp
randomised nucleotides 9-12
ra010
raPCR_AS13-has-mir4286
cactgaatccaactgggtaccaggagtggggtgcatacatggactgc
has-mir4286
13 bp
ra011
raPCR_AS13-mm-mir-375
cactgaatccaactgtcacgcgagccgaacgaacaaagcatacatggactgc
mm-mir-375
13 bp
ra012
raPCR_AS13-mm-mir-375(ran9-12)
cactgaatccaactgtcacgcgagcnnnncgaacaaagcatacatggactgc
mm-mir-375 (ran9-12)
13 bp
randomised nucleotides 9-12
ra013
raPCR_AS13-mm-mir-376a
cactgaatccaactgacgtggattttcctctacgatgcatacatggactgc
mm-mir-376a
13 bp
ra014
raPCR_AS13-spacer(0)
cagttggattcagtggcagtccatgtatgc
spacer
13 bp
ra015
raPCR_AS13-spacer(10)
cagttggattcagtggctatttctcgcagtccatgtatgc
spacer
13 bp
ra016
raPCR_AS13-spacer(20)
cagttggattcagtgatgacaggtagctatttctcgcagtccatgtatgc
spacer
13 bp
ra017
raPCR_AS13-stop_fw_BBB
ttttgaattcgcggccgcactagtcactgaatccaactg
stop fw
13 bp
ra018
raPCR_AS13-stop_rev_BBB
ttttctgcagcggccgcgctagcgcagtccatgtatgc
stop rev
13 bp
ra019
raPCR_AS13-stop_rev_NotI
ttttgcggccgctggagtgtgacaatggtgtttg
stop rev
13 bp
ra020
raPCR_AS13-stop_fw_XhoI
ttttctcgagcactgaatccaactg
stop fw
13 bp
Maus oligos
ID
Name
Sequence (5' to 3')
Synthesis scale
mouse1
CMV_TetR_122bs_SalI_fw
ttttgtcgaccgttgacattgattattgac
25N
mouae2
CMV_TetR_both_MluI_rev
ttttacgcgttaacacacaaaaaaccaacac
25N
mouse3
CMV_TetR_control_SalI_fw
ttttgtcgaccgttgacattgattattgac
25N
mouse4
CMV_TetO2_EcoRI_fw
ttttgaattcgttgacattgattattgtctag
25N
mouse5
Luc2_Ter_PstI_rev
ttttctgcagtaccacatttgtagaggttttac
25N
mouse6
haat_bs_p_fw
tcgaggaagcgtttaggcatgtttaacatctctagagc
25N
mouse7
haat_bs_p_rev
ggccgctctagagatgttaaacatgcctaaacgcttcc
25N
mouse8
haat_bs_r10_12_aat_fw
tcgaggaagcgtaatggcatgtttaacatctctagagc
25N
mouse9
haat_bs_r10_12_aat_rev
ggccgctctagagatgttaaacatgccattacgcttcc
25N
mouse10
haat_bs_r10_12_agc_fw
tcgaggaagcgtagcggcatgtttaacatctctagagc
25N
mouse11
haat_bs_r10_12_agc_rev
ggccgctctagagatgttaaacatgccgctacgcttcc
25N
mouse12
haat_bs_m10at_fw
tcgaggaagcgttttggcatgtttaacatctctagagc
25N
mouse13
haat_bs_m10at_rev
ggccgctctagagatgttaaacatgccaaaacgcttcc
25N
mouse14
haat_bs_m10ac_fw
tcgaggaagcgtttcggcatgtttaacatctctagagc
25N
mouse15
haat_bs_m10ac_rev
ggccgctctagagatgttaaacatgccgaaacgcttcc
25N
mouse16
haat_bs_m11ta_fw
tcgaggaagcgttaaggcatgtttaacatctctagagc
25N
mouse17
haat_bs_m11ta_rev
ggccgctctagagatgttaaacatgccttaacgcttcc
25N
mouse18
haat_bs_m11tg_fw
tcgaggaagcgttgaggcatgtttaacatctctagagc
25N
mouse19
haat_bs_m11tg_rev
ggccgctctagagatgttaaacatgcctcaacgcttcc
25N
mouse20
haat_bs_r9_12_aatc_fw
tcgaggaagcgtaatcgcatgtttaacatctctagagc
25N
mouse21
haat_bs_r9_12_aatc_rev
ggccgctctagagatgttaaacatgcgattacgcttcc
25N
mouse22
haat_bs_r9_12_agcc_fw
tcgaggaagcgtagccgcatgtttaacatctctagagc
25N
mouse23
haat_bs_r9_12_agcc_rev
ggccgctctagagatgttaaacatgcggctacgcttcc
25N
mouse24
haat_bs_onlyseed_fw
tcgagcttcgcaaatcgcatgtttaacatctctagagc
25N
mouse25
haat_bs_onlyseed_rev
ggccgctctagagatgttaaacatgcgatttgcgaagc
25N
mouse26
haat_bs_r16-18_fw
tcgaggttccgtttaggcatgtttaacatctctagagc
25N
mouse27
haat_bs_r16-18_rev
ggccgctctagagatgttaaacatgcctaaacggaacc
25N
mouse28
sAg_bs_p_fw
tcgagctcagtttactagtgccatttgttctctagagc
25N
mouse29
sAg_bs_p_rev
ggccgctctagagaacaaatggcactagtaaactgagc
25N
mouse30
sAg_bs_r10_12_acg_fw
tcgagctcagtttactagacgcatttgttctctagagc
25N
mouse31
sAg_bs_r10_12_acg_rev
ggccgctctagagaacaaatgcgtctagtaaactgagc
25N
mouse32
sAg_bs_r10_12_taa_fw
tcgagctcagtttactagtaacatttgttctctagagc
25N
mouse33
sAg_bs_r10_12_taa_rev
ggccgctctagagaacaaatgttactagtaaactgagc
25N
mouse34
sAg_bs_r9_12_acgg_fw
tcgagctcagtttactagacggatttgttctctagagc
25N
mouse35
sAg_bs_r9_12_acgg_rev
ggccgctctagagaacaaatccgtctagtaaactgagc
25N
mouse36
sAg_bs_r9_12_atgt_fw
tcgagctcagtttactagatgtatttgttctctagagc
25N
mouse37
sAg_bs_r9_12_atgt_rev
ggccgctctagagaacaaatacatctagtaaactgagc
25N
mouse38
sAg_bs_m10cg_fw
tcgagctcagtttactagtggcatttgttctctagagc
25N
mouse39
sAg_bs_m10cg_rev
ggccgctctagagaacaaatgccactagtaaactgagc
25N
mouse40
sAg_bs_m10ca_fw
tcgagctcagtttactagtgacatttgttctctagagc
25N
mouse41
sAg_bs_m10ca_rev
ggccgctctagagaacaaatgtcactagtaaactgagc
25N
mouse42
sAg_bs_m11gc_fw
tcgagctcagtttactagtcccatttgttctctagagc
25N
mouse43
sAg_bs_m11gc_rev
ggccgctctagagaacaaatgggactagtaaactgagc
25N
mouse44
sAg_bs_m11ga_fw
tcgagctcagtttactagtaccatttgttctctagagc
25N
mouse45
sAg_bs_m11ga_rev
ggccgctctagagaacaaatggtactagtaaactgagc
25N
mouse46
sAg_bs_onlyseed_fw
tcgagctcagtaatgatcacggatttgttctctagagc
25N
mouse47
sAg_bs_onlyseed_rev
ggccgctctagagaacaaatccgtgatcattactgagc
25N
mouse48
sAg_bs_r16-18_fw
tcgagctcagttatgtagtgccatttgttctctagagc
25N
mouse49
sAg_bs_r16-18_rev
ggccgctctagagaacaaatggcactacataactgagc
25N
mouse50
hAAT_cDNA_SalI_Bluescript_fw
ttttgtcgacgaccatgattacgccaagcg
25N
mouse51
hAAT_cDNA_SalI_fw
ttttgtcgacgggtaccgggccccccgtcgaggatg
25N
mouse52
hAAT_cDNA_XhoI_NotI_NheI_rev
ttttgctagcgcggccgcgtatctcgaggctcaacccttctttaatg
25N
mouse53
hAAT_cDNA_sAg_bs_p_NheI_rev
ttttgctagcgaacaaatggcactagtaaactgaggctcaacccttctttaatg
25N
mouse54
hAAT_cDNA_sAg_bs_r10_12_acg_NheI_rev
ttttgctagcgaacaaatgcgtctagtaaactgaggctcaacccttctttaatg
25N
mouse55
hAAT_cDNA_sAg_bs_r10_12_taa_NheI_rev
ttttgctagcgaacaaatgttactagtaaactgaggctcaacccttctttaatg
25N
mouse56
hAAT_cDNA_bs_r9_12_acgg_NheI_rev
ttttgctagcgaacaaatccgtctagtaaactgaggctcaacccttctttaatg
25N
mouse57
hAAT_cDNA_sAg_bs_r9_12_atgt_NheI_rev
ttttgctagcgaacaaatacatctagtaaactgaggctcaacccttctttaatg
25N
mouse58
hAAT_cDNA_sAg_bs_m10cg_NheI_rev
ttttgctagcgaacaaatgccactagtaaactgaggctcaacccttctttaatg
25N
mouse59
hAAT_cDNA_sAg_bs_m10ca_NheI_rev
ttttgctagcgaacaaatgtcactagtaaactgaggctcaacccttctttaatg
25N
mouse60
hAAT_cDNA_sAg_bs_m11gc_NheI_rev
ttttgctagcgaacaaatgggactagtaaactgaggctcaacccttctttaatg
25N
mouse61
hAAT_cDNA_sAg_bs_m11ga_NheI_rev
ttttgctagcgaacaaatggtactagtaaactgaggctcaacccttctttaatg
25N
mouse62
hAAT_cDNA_sAg_bs_onlyseed_NheI_rev
ttttgctagcgaacaaatccgtgatcattactgaggctcaacccttctttaatg
25N
mouse63
hAAT_cDNA_sAg_bs_r16-18_NheI_rev
ttttgctagcgaacaaatggcactacataactgaggctcaacccttctttaatg
25N
mouse64
sAg_bs_p_fw_E
aattcctcagtttactagtgccatttgttcctgca
25N
mouse65
sAg_bs_p_rev_P
ggaacaaatggcactagtaaactgagg
25N
mouse66
sAg_bs_r10_12_acg_fw_E
aattcctcagtttactagacgcatttgttcctgca
25N
mouse67
sAg_bs_r10_12_acg_rev_P
ggaacaaatgcgtctagtaaactgagg
25N
mouse68
sAg_bs_r10_12_taa_fw_E
aattcctcagtttactagtaacatttgttcctgca
25N
mouse69
sAg_bs_r10_12_taa_rev_P
ggaacaaatgttactagtaaactgagg
25N
mouse70
sAg_bs_r9_12_acgg_fw_E
aattcctcagtttactagacggatttgttcctgca
25N
mouse71
sAg_bs_r9_12_acgg_rev_P
ggaacaaatccgtctagtaaactgagg
25N
mouse72
sAg_bs_r9_12_atgt_fw_E
aattcctcagtttactagatgtatttgttcctgca
25N
mouse73
sAg_bs_r9_12_atgt_rev_P
ggaacaaatacatctagtaaactgagg
25N
mouse74
sAg_bs_m10cg_fw_E
aattcctcagtttactagtggcatttgttcctgca
25N
mouse75
sAg_bs_m10cg_rev_P
ggaacaaatgccactagtaaactgagg
25N
mouse76
sAg_bs_m10ca_fw_E
aattcctcagtttactagtgacatttgttcctgca
25N
mouse77
sAg_bs_m10ca_rev_P
ggaacaaatgtcactagtaaactgagg
25N
mouse78
sAg_bs_m11gc_fw_E
aattcctcagtttactagtcccatttgttcctgca
25N
mouse79
sAg_bs_m11gc_rev_P
ggaacaaatgggactagtaaactgagg
25N
mouse80
sAg_bs_m11ga_fw_E
aattcctcagtttactagtaccatttgttcctgca
25N
mouse81
sAg_bs_m11ga_rev_P
ggaacaaatggtactagtaaactgagg
25N
mouse82
sAg_bs_onlyseed_fw_E
aattcctcagtaatgatcacggatttgttcctgca
25N
mouse83
sAg_bs_onlyseed_rev_P
ggaacaaatccgtgatcattactgagg
25N
mouse84
sAg_bs_r16-18_fw_E
aattcctcagttatgtagtgccatttgttcctgca
25N
mouse85
sAg_bs_r16-18_rev_P
ggaacaaatggcactacataactgagg
25N