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Team:TU Munich/Software
From 2010.igem.org
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MotivationThe intention of this software is to show the full potential of our logical RNA devices which could not yet been demonstrated in the lab. Thus so far we only studied single switches in the lab, the full power of our logical RNA devices lies in the interconnection between several switches and in the ability to couple them to cellular information pathways like RNA pathways and transcription factors. The applet illustrates exemplary how our logical RNA devices could be used in the future. Program
TutorialThe program consists of three modules: Design, Check and Turn-to-DNA. The first allows the user to enter inputs, whereas the second and third supply the user with information about his network.
Check: This module illustrates in the colors green for true and red for false which nods and relations are assigned the values 'true' or 'false'. Turn-to-DNA: This module assigns the abstract logic network concrete sequences, e.g. terminator sequences, generates random sequences and provides all the genetic information to rebuild the network. The different modules are described in detail below. 1. Design a logic network
A logic network can be designed by adding logical devices (via double clicking) and interconnecting them. There are five different types of logical devices: inputs, outputs, AND-gates, OR-gates and NOT-gates. The type of a local device can be change by clicking on the combo box on the device and selecting a different type. Logical devices can be interconnected by selecting (via clicking inside the area of a device) two devices and then pressing the C key (for connect). A complete logic network has to have at least one input, at least one output, and at least one gate (AND, OR, NOT). Different devices may require different numbers of ingoing and outgoing connections (e.g. a NOT-gate requires one ingoing connection and allows for a arbitrary number of outgoing connections, whereas the AND-gate requires two ingoing connections and allows for a arbitrary number of outgoing connections; an input may not have an incoming connection, an output no outgoing connection). In addition the logic network may not include loops. The user has to assign DNA sequences (either custom RNA sequences or sequences coding for proteins) to all input and output devices by clicking on the "Set input" button of the devices.
2. Check the logic network
The logic network designed in step 1 can now be verified. Logical values are represented by colors (green = true, red = false). Thus a green box represents a devices (e.g. an AND-gate) with the logical value true, a red box a devices with the logical value false. The flow of logical value can be followed by following the colored edges between the boxes. The logical value(s) of the input(s) can be manipulated by clicking on their checkbox(es). Close
3. Turn the logic network into DNA
A set of genetic devices (categorized as input elements, switch elements and output elements) representing the logical network designed in step 1 is calculated and displayed. All elements consist of an Escherichia coli promoter, a Ribosome Binding Site, up to two switches, one output sequences and a terminator. CloseExamplesA simple NOT-gate
NOT CloseAn AND-gate
AND CloseCommon mistakes
COMMON MISTAKES CloseDisclaimerSoftwareTHIS SOFTWARE IS PROVIDED BY THE CONTRIBUTORS "AS IS" AND ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE ARE DISCLAIMED. IN NO EVENT SHALL THE CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES (INCLUDING, BUT NOT LIMITED TO, PROCUREMENT OF SUBSTITUTE GOODS OR SERVICES; LOSS OF USE, DATA, OR PROFITS; OR BUSINESS INTERRUPTION) HOWEVER CAUSED AND ON ANY THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE OF THIS SOFTWARE, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE.
BiologyTHIS SOFTWARE IS FOR DEMONSTRATION PURPOSES ONLY. THE WARRANTY OF FITNESS FOR A PARTICULAR PURPOSE, INCLUDING THE DESIGN OF FUNCTIONAL GENE CIRCUITS, IS DISCLAIMED. ESPECIALLY DO NOT USE THE SOFTWARE IN THE PROCESS OF DESIGNING REAL EXPERIMENTS OR ORDERING GENETIC DEVICES / SEQUENCES.
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