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Home The Team The Project Parts Submitted to the Registry Modeling Notebook Safety

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Contents 1 Notebook 1.1 6/16/10 1.2 6/21/10 1.3 6/23/10 1.4 6/24/10 1.5 6/25/10

Notebook

6/16/10

Prepared nine pots of wildtype (Col-0) A. thaliana and moved to cold storage for stratification. Pots intended for use in Agrobacterium flower-dip. Seeds were sewn in soilless media and the pot was covered with a wide meshing to retain the soil.

6/21/10

Transfered pots from cold storage to greenhouse. Prepared 0.5 MS agar media and sterilized seeds for sterile growth, to be sewn on 6/23. Seeds left in dilute agar solution in fridge to stratify.

6/23/10

Agar plates poured. Seeds to be sewn on 6/24 to give time for the media to solidify.

6/24/10

Seeds sown onto agar. Five large culture plates and two small plates were then transfered into horticulture growth chamber. Seeds in soil media forecasted to germinate by 6/26.

6/25/10

A. thaliana expirement mapping: Testing low oxygen response in WT and Transgenic using atmospheric chamber in Purdue Agronomy department. Using sensors to measure ethanol, oxygen, auxin response in control and in hypoxic conditions.